Quantitative Determination of Isosorbide Dinitrate and Two Metabolites in Plasma
A GLC method for the determination of plasma isosorbide dinitrate and two metabolites, isosorbide 2-mononitrate and isosorbide 5-mononitrate, is described. The three substances are extracted from alkalinized plasma with ether. Quantitation is effected by electrón-capture detection after GLC separati...
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| Veröffentlicht in: | Journal of pharmaceutical sciences 1977-08, Vol.66 (8), p.1143-1145 |
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| container_title | Journal of pharmaceutical sciences |
| container_volume | 66 |
| creator | Chin, David A. Prue, David G. Michelucci, John Kho, B.T. Warner, Charles R. |
| description | A GLC method for the determination of plasma isosorbide dinitrate and two metabolites, isosorbide 2-mononitrate and isosorbide 5-mononitrate, is described. The three substances are extracted from alkalinized plasma with ether. Quantitation is effected by electrón-capture detection after GLC separation with a 30% SE-30 column. The unusually heavy liquid phase loading is necessary to eliminate irreversible adsorption on the solid support. The electron-capture detector provides excellent sensitivity and specificity because of the electronegative nature of the nitric ester. The method was used to study the blood levels of isosorbide dinitrate and two metabolites in four beagle dogs after single oral doses of 40mg of isosorbide dinitrate and in two human volunteers after a sublingual dose of 10mg of isosorbide dinitrate. |
| doi_str_mv | 10.1002/jps.2600660823 |
| format | Article |
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The three substances are extracted from alkalinized plasma with ether. Quantitation is effected by electrón-capture detection after GLC separation with a 30% SE-30 column. The unusually heavy liquid phase loading is necessary to eliminate irreversible adsorption on the solid support. The electron-capture detector provides excellent sensitivity and specificity because of the electronegative nature of the nitric ester. The method was used to study the blood levels of isosorbide dinitrate and two metabolites in four beagle dogs after single oral doses of 40mg of isosorbide dinitrate and in two human volunteers after a sublingual dose of 10mg of isosorbide dinitrate.</description><identifier>ISSN: 0022-3549</identifier><identifier>EISSN: 1520-6017</identifier><identifier>DOI: 10.1002/jps.2600660823</identifier><identifier>PMID: 894502</identifier><language>eng</language><publisher>Washington: Elsevier Inc</publisher><subject>Administration, Oral ; Animals ; Chromatography, Gas ; coronary-isosorbide dinitrate and metabolites ; Dogs ; electron capture-analysis ; electron-capture GLC analysis ; GLC ; GLC electron capture—analysis, isosorbide dinitrate and metabolites, plasma ; Humans ; Isosorbide Dinitrate - administration & dosage ; Isosorbide Dinitrate - blood ; Isosorbide dinitrate-and metabolites ; Isosorbide dinitrate—and metabolites electron-capture GLC analysis, plasma ; Methods ; Mouth Floor ; plasma ; Tablets ; Time Factors ; Vasodilators ; Vasodilators coronary—isosorbide dinitrate and metabolites, electron-capture GLC analysis, plasma</subject><ispartof>Journal of pharmaceutical sciences, 1977-08, Vol.66 (8), p.1143-1145</ispartof><rights>1977 Wiley-Liss, Inc., A Wiley Company</rights><rights>Copyright © 1977 Wiley‐Liss, Inc., A Wiley Company</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3413-50e82c2bc2048e8f889ce5b398e6da36b697a8d05036e9b6fe1063477c58af3f3</citedby><cites>FETCH-LOGICAL-c3413-50e82c2bc2048e8f889ce5b398e6da36b697a8d05036e9b6fe1063477c58af3f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjps.2600660823$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjps.2600660823$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/894502$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chin, David A.</creatorcontrib><creatorcontrib>Prue, David G.</creatorcontrib><creatorcontrib>Michelucci, John</creatorcontrib><creatorcontrib>Kho, B.T.</creatorcontrib><creatorcontrib>Warner, Charles R.</creatorcontrib><title>Quantitative Determination of Isosorbide Dinitrate and Two Metabolites in Plasma</title><title>Journal of pharmaceutical sciences</title><addtitle>J. Pharm. Sci</addtitle><description>A GLC method for the determination of plasma isosorbide dinitrate and two metabolites, isosorbide 2-mononitrate and isosorbide 5-mononitrate, is described. The three substances are extracted from alkalinized plasma with ether. Quantitation is effected by electrón-capture detection after GLC separation with a 30% SE-30 column. The unusually heavy liquid phase loading is necessary to eliminate irreversible adsorption on the solid support. The electron-capture detector provides excellent sensitivity and specificity because of the electronegative nature of the nitric ester. The method was used to study the blood levels of isosorbide dinitrate and two metabolites in four beagle dogs after single oral doses of 40mg of isosorbide dinitrate and in two human volunteers after a sublingual dose of 10mg of isosorbide dinitrate.</description><subject>Administration, Oral</subject><subject>Animals</subject><subject>Chromatography, Gas</subject><subject>coronary-isosorbide dinitrate and metabolites</subject><subject>Dogs</subject><subject>electron capture-analysis</subject><subject>electron-capture GLC analysis</subject><subject>GLC</subject><subject>GLC electron capture—analysis, isosorbide dinitrate and metabolites, plasma</subject><subject>Humans</subject><subject>Isosorbide Dinitrate - administration & dosage</subject><subject>Isosorbide Dinitrate - blood</subject><subject>Isosorbide dinitrate-and metabolites</subject><subject>Isosorbide dinitrate—and metabolites electron-capture GLC analysis, plasma</subject><subject>Methods</subject><subject>Mouth Floor</subject><subject>plasma</subject><subject>Tablets</subject><subject>Time Factors</subject><subject>Vasodilators</subject><subject>Vasodilators coronary—isosorbide dinitrate and metabolites, electron-capture GLC analysis, plasma</subject><issn>0022-3549</issn><issn>1520-6017</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1977</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1P3DAURa2qlA7QLSsWWbHL8GLHjrNEtMOHoJ0Wqi4tx3mRTJN4sD0M_HtcBVGxQKws6557ZR9C9guYFwD06HYV5lQACAGSsg9kVnAKuYCi-khmCaA542X9meyEcAsJA863ySdZlxzojCx_rvUYbdTR3mP2FSP6wY7p5sbMddl5cMH5xrYps6ONXkfM9NhmNxuXXWHUjettxJDZMVv2Ogx6j2x1ug_45fncJb8X325OzvLLH6fnJ8eXuWFlwXIOKKmhjaFQSpSdlLVB3rBaomg1E42oKy1b4MAE1o3osADByqoyXOqOdWyXHE67K-_u1hiiGmww2Pd6RLcOSpbAWcXLBM4n0HgXgsdOrbwdtH9UBah_BlUyqP4bTIWD5-V1M2D7gk_KUlxP8cb2-PjOmLpYXr-azqeuDREfXrra_1WiSq9Vf76fqmKx_FXVUKpF4uXEYzJ5b9GrYCyOBlvr0UTVOvvWL54ALUqeXA</recordid><startdate>197708</startdate><enddate>197708</enddate><creator>Chin, David A.</creator><creator>Prue, David G.</creator><creator>Michelucci, John</creator><creator>Kho, B.T.</creator><creator>Warner, Charles R.</creator><general>Elsevier Inc</general><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197708</creationdate><title>Quantitative Determination of Isosorbide Dinitrate and Two Metabolites in Plasma</title><author>Chin, David A. ; Prue, David G. ; Michelucci, John ; Kho, B.T. ; Warner, Charles R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3413-50e82c2bc2048e8f889ce5b398e6da36b697a8d05036e9b6fe1063477c58af3f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1977</creationdate><topic>Administration, Oral</topic><topic>Animals</topic><topic>Chromatography, Gas</topic><topic>coronary-isosorbide dinitrate and metabolites</topic><topic>Dogs</topic><topic>electron capture-analysis</topic><topic>electron-capture GLC analysis</topic><topic>GLC</topic><topic>GLC electron capture—analysis, isosorbide dinitrate and metabolites, plasma</topic><topic>Humans</topic><topic>Isosorbide Dinitrate - administration & dosage</topic><topic>Isosorbide Dinitrate - blood</topic><topic>Isosorbide dinitrate-and metabolites</topic><topic>Isosorbide dinitrate—and metabolites electron-capture GLC analysis, plasma</topic><topic>Methods</topic><topic>Mouth Floor</topic><topic>plasma</topic><topic>Tablets</topic><topic>Time Factors</topic><topic>Vasodilators</topic><topic>Vasodilators coronary—isosorbide dinitrate and metabolites, electron-capture GLC analysis, plasma</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chin, David A.</creatorcontrib><creatorcontrib>Prue, David G.</creatorcontrib><creatorcontrib>Michelucci, John</creatorcontrib><creatorcontrib>Kho, B.T.</creatorcontrib><creatorcontrib>Warner, Charles R.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of pharmaceutical sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chin, David A.</au><au>Prue, David G.</au><au>Michelucci, John</au><au>Kho, B.T.</au><au>Warner, Charles R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantitative Determination of Isosorbide Dinitrate and Two Metabolites in Plasma</atitle><jtitle>Journal of pharmaceutical sciences</jtitle><addtitle>J. Pharm. Sci</addtitle><date>1977-08</date><risdate>1977</risdate><volume>66</volume><issue>8</issue><spage>1143</spage><epage>1145</epage><pages>1143-1145</pages><issn>0022-3549</issn><eissn>1520-6017</eissn><abstract>A GLC method for the determination of plasma isosorbide dinitrate and two metabolites, isosorbide 2-mononitrate and isosorbide 5-mononitrate, is described. The three substances are extracted from alkalinized plasma with ether. Quantitation is effected by electrón-capture detection after GLC separation with a 30% SE-30 column. The unusually heavy liquid phase loading is necessary to eliminate irreversible adsorption on the solid support. The electron-capture detector provides excellent sensitivity and specificity because of the electronegative nature of the nitric ester. The method was used to study the blood levels of isosorbide dinitrate and two metabolites in four beagle dogs after single oral doses of 40mg of isosorbide dinitrate and in two human volunteers after a sublingual dose of 10mg of isosorbide dinitrate.</abstract><cop>Washington</cop><pub>Elsevier Inc</pub><pmid>894502</pmid><doi>10.1002/jps.2600660823</doi><tpages>3</tpages></addata></record> |
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| ispartof | Journal of pharmaceutical sciences, 1977-08, Vol.66 (8), p.1143-1145 |
| issn | 0022-3549 1520-6017 |
| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Alma/SFX Local Collection |
| subjects | Administration, Oral Animals Chromatography, Gas coronary-isosorbide dinitrate and metabolites Dogs electron capture-analysis electron-capture GLC analysis GLC GLC electron capture—analysis, isosorbide dinitrate and metabolites, plasma Humans Isosorbide Dinitrate - administration & dosage Isosorbide Dinitrate - blood Isosorbide dinitrate-and metabolites Isosorbide dinitrate—and metabolites electron-capture GLC analysis, plasma Methods Mouth Floor plasma Tablets Time Factors Vasodilators Vasodilators coronary—isosorbide dinitrate and metabolites, electron-capture GLC analysis, plasma |
| title | Quantitative Determination of Isosorbide Dinitrate and Two Metabolites in Plasma |
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