Enzyme Multiplied Immunoassay of Phenobarbital by LKB Reaction Rate Analyser: A Comparison with Analysis by Gas-Liquid Chromatography

Enzyme Multiplied Immunoassay of Phenobarbital by LKB Reaction Rate Analyser: A Comparison with Analysis by Gas-Liquid Chromatography

A procedure for the enzyme multiplied immunoassay of phenobarbital using the LKB Reaction Rate Analyser is described. The temperature optimum, linearity, specificity, and precision of the assay are reported. A highly significant correlation coefficient is obtained when the technique is compared with...

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Veröffentlicht in:Annals of clinical biochemistry 1977, Vol.14 (1-6), p.218-222
Hauptverfasser: Belfield, Alan, Duncan, Anne M., Reavey, P. C.
Format: Artikel
Sprache:eng
Schlagworte:
Autoanalysis
Chromatography, Gas
Evaluation Studies as Topic
Glucosephosphate Dehydrogenase
Humans
Immunoenzyme Techniques
Kinetics
Leuconostoc - enzymology
Phenobarbital - blood
Temperature
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container_issue 1-6
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container_title Annals of clinical biochemistry
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creator Belfield, Alan
Duncan, Anne M.
Reavey, P. C.
description A procedure for the enzyme multiplied immunoassay of phenobarbital using the LKB Reaction Rate Analyser is described. The temperature optimum, linearity, specificity, and precision of the assay are reported. A highly significant correlation coefficient is obtained when the technique is compared with gas-liquid chromatography by analysing human sera containing a range of phenobarbital levels.
doi_str_mv 10.1177/000456327701400159
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source MEDLINE; Alma/SFX Local Collection
subjects Autoanalysis
Chromatography, Gas
Evaluation Studies as Topic
Glucosephosphate Dehydrogenase
Humans
Immunoenzyme Techniques
Kinetics
Leuconostoc - enzymology
Phenobarbital - blood
Temperature
title Enzyme Multiplied Immunoassay of Phenobarbital by LKB Reaction Rate Analyser: A Comparison with Analysis by Gas-Liquid Chromatography
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