Cytophotometric determination of basic proteins with heparin-alcian blue method
For the quantitative cyfcochemical determination of basic proteins, Heparin-Alcian Blue (HAB-was found to be a more suitable staining procedure than the generally used alcalic Fast green reaction. HAB is able to stain all the histone fractions, but there is no staining following deamination (gelelec...
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| Veröffentlicht in: | Acta histochemica 1977, Vol.58 (2), p.203-209 |
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| container_title | Acta histochemica |
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| creator | Pokorny, Éva Szentirmay, Zoltán Sugár, János |
| description | For the quantitative cyfcochemical determination of basic proteins, Heparin-Alcian Blue (HAB-was found to be a more suitable staining procedure than the generally used alcalic Fast green reaction. HAB is able to stain all the histone fractions, but there is no staining following deamination (gelelectrophoretic examinations) and no aspecific staining (Millipore filter model). In
Ehrlich ascites tumour cells, changes in the
Feulgen-DNA. content is followed by the proportional changes of HAB-basic protein content. After HAB reaction, the
Feulgen reaction can be performed on the same slide. |
| doi_str_mv | 10.1016/S0065-1281(77)80130-X |
| format | Article |
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Ehrlich ascites tumour cells, changes in the
Feulgen-DNA. content is followed by the proportional changes of HAB-basic protein content. After HAB reaction, the
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Ehrlich ascites tumour cells, changes in the
Feulgen-DNA. content is followed by the proportional changes of HAB-basic protein content. After HAB reaction, the
Feulgen reaction can be performed on the same slide.</description><subject>Alcian Blue</subject><subject>Animals</subject><subject>Carcinoma, Ehrlich Tumor - analysis</subject><subject>DNA, Neoplasm - analysis</subject><subject>Heparin</subject><subject>Histocytochemistry</subject><subject>Histones - analysis</subject><subject>Mice</subject><subject>Neoplasm Proteins - analysis</subject><subject>Rats</subject><subject>T-Lymphocytes - analysis</subject><issn>0065-1281</issn><issn>1618-0372</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1977</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMlOwzAQhi3EVpY3ACknBIfAOE5s94RQxSZV4gBIvVmOPVGNkrjYLqhvT9oirpxmNPP_s3yEnFO4pkD5zSsAr3JaSHopxJUEyiCf7ZAR5VTmwESxS0Z_kkNyFOMHAIyBFQdkv1wnYkReJqvkF3OffIcpOJNZTBg61-vkfJ_5Jqt1HMqL4BO6PmbfLs2zOS50cH2uW-N0n9XtErPBP_f2hOw1uo14-huPyfvD_dvkKZ--PD5P7qa5YSBSbkWtARvRWFYg1FpLYONC8srwsgaNkmsLjDVSQllzThnDhsmSI6_G0hpkx-RiO3c47HOJManORYNtq3v0y6hkCaWAohqE1VZogo8xYKMWwXU6rBQFteaoNhzVGpISQm04qtngO_tdsKw7tH-uLbihfbtt4_Dkl8OgonHYG7QuoEnKevfPgh_D54Nz</recordid><startdate>1977</startdate><enddate>1977</enddate><creator>Pokorny, Éva</creator><creator>Szentirmay, Zoltán</creator><creator>Sugár, János</creator><general>Elsevier GmbH</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1977</creationdate><title>Cytophotometric determination of basic proteins with heparin-alcian blue method</title><author>Pokorny, Éva ; Szentirmay, Zoltán ; Sugár, János</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c307t-d7ba0ef7fd32e0baa80392865c64b0ae86ad033f8804b66133ef3846e6598dce3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1977</creationdate><topic>Alcian Blue</topic><topic>Animals</topic><topic>Carcinoma, Ehrlich Tumor - analysis</topic><topic>DNA, Neoplasm - analysis</topic><topic>Heparin</topic><topic>Histocytochemistry</topic><topic>Histones - analysis</topic><topic>Mice</topic><topic>Neoplasm Proteins - analysis</topic><topic>Rats</topic><topic>T-Lymphocytes - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pokorny, Éva</creatorcontrib><creatorcontrib>Szentirmay, Zoltán</creatorcontrib><creatorcontrib>Sugár, János</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Acta histochemica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pokorny, Éva</au><au>Szentirmay, Zoltán</au><au>Sugár, János</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cytophotometric determination of basic proteins with heparin-alcian blue method</atitle><jtitle>Acta histochemica</jtitle><addtitle>Acta Histochem</addtitle><date>1977</date><risdate>1977</risdate><volume>58</volume><issue>2</issue><spage>203</spage><epage>209</epage><pages>203-209</pages><issn>0065-1281</issn><eissn>1618-0372</eissn><abstract>For the quantitative cyfcochemical determination of basic proteins, Heparin-Alcian Blue (HAB-was found to be a more suitable staining procedure than the generally used alcalic Fast green reaction. HAB is able to stain all the histone fractions, but there is no staining following deamination (gelelectrophoretic examinations) and no aspecific staining (Millipore filter model). In
Ehrlich ascites tumour cells, changes in the
Feulgen-DNA. content is followed by the proportional changes of HAB-basic protein content. After HAB reaction, the
Feulgen reaction can be performed on the same slide.</abstract><cop>Germany</cop><pub>Elsevier GmbH</pub><pmid>409037</pmid><doi>10.1016/S0065-1281(77)80130-X</doi><tpages>7</tpages></addata></record> |
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| identifier | ISSN: 0065-1281 |
| ispartof | Acta histochemica, 1977, Vol.58 (2), p.203-209 |
| issn | 0065-1281 1618-0372 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Alcian Blue Animals Carcinoma, Ehrlich Tumor - analysis DNA, Neoplasm - analysis Heparin Histocytochemistry Histones - analysis Mice Neoplasm Proteins - analysis Rats T-Lymphocytes - analysis |
| title | Cytophotometric determination of basic proteins with heparin-alcian blue method |
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