A more sensitive modification of the catalase assay with the Clark oxygen electrode: Application to the kinetic study of the pea leaf enzyme
A modification of the method of catalase determination by means of the Clark oxygen electrode is described. The assay is based on measurement of the initial rate at which oxygen is released by catalase in an oxygen-free buffer. Displacement of oxygen was brought about by flushing with nitrogen, and...
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| Veröffentlicht in: | Analytical biochemistry 1977-06, Vol.80 (2), p.409-415 |
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| container_title | Analytical biochemistry |
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| creator | del Río, Luís A. Ortega, M.Gómez López, A.Leal Gorgé, J.López |
| description | A modification of the method of catalase determination by means of the Clark oxygen electrode is described. The assay is based on measurement of the initial rate at which oxygen is released by catalase in an oxygen-free buffer. Displacement of oxygen was brought about by flushing with nitrogen, and the substrate used was hydrogen peroxide at a 33.5 m
m final concentration. The method is rapid and can be used with crude catalase preparations. Its sensitivity is at least 20 times higher than that of previous methods; it has an interval of measurable activity of about 0.01–8.4 μmol of O
2/min and, therefore, is applicable to an 840-fold range of catalase concentrations. This modification was applied to the kinetic study of crude extracts of pea leaf catalase. An apparent
K
m
of 0.190
m was calculated. |
| doi_str_mv | 10.1016/0003-2697(77)90662-5 |
| format | Article |
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m final concentration. The method is rapid and can be used with crude catalase preparations. Its sensitivity is at least 20 times higher than that of previous methods; it has an interval of measurable activity of about 0.01–8.4 μmol of O
2/min and, therefore, is applicable to an 840-fold range of catalase concentrations. This modification was applied to the kinetic study of crude extracts of pea leaf catalase. An apparent
K
m
of 0.190
m was calculated.</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1016/0003-2697(77)90662-5</identifier><identifier>PMID: 889081</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Catalase - analysis ; Electrodes ; Evaluation Studies as Topic ; Kinetics ; Methods ; Microchemistry ; Oxygen ; Plants - enzymology</subject><ispartof>Analytical biochemistry, 1977-06, Vol.80 (2), p.409-415</ispartof><rights>1977</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0003-2697(77)90662-5$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/889081$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>del Río, Luís A.</creatorcontrib><creatorcontrib>Ortega, M.Gómez</creatorcontrib><creatorcontrib>López, A.Leal</creatorcontrib><creatorcontrib>Gorgé, J.López</creatorcontrib><title>A more sensitive modification of the catalase assay with the Clark oxygen electrode: Application to the kinetic study of the pea leaf enzyme</title><title>Analytical biochemistry</title><addtitle>Anal Biochem</addtitle><description>A modification of the method of catalase determination by means of the Clark oxygen electrode is described. The assay is based on measurement of the initial rate at which oxygen is released by catalase in an oxygen-free buffer. Displacement of oxygen was brought about by flushing with nitrogen, and the substrate used was hydrogen peroxide at a 33.5 m
m final concentration. The method is rapid and can be used with crude catalase preparations. Its sensitivity is at least 20 times higher than that of previous methods; it has an interval of measurable activity of about 0.01–8.4 μmol of O
2/min and, therefore, is applicable to an 840-fold range of catalase concentrations. This modification was applied to the kinetic study of crude extracts of pea leaf catalase. An apparent
K
m
of 0.190
m was calculated.</description><subject>Catalase - analysis</subject><subject>Electrodes</subject><subject>Evaluation Studies as Topic</subject><subject>Kinetics</subject><subject>Methods</subject><subject>Microchemistry</subject><subject>Oxygen</subject><subject>Plants - enzymology</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1977</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kU9P3DAQxa2q_7bQb8DBpwoOacfOJrZ7QFqtaEFC6qWcrVlnXFySOI29QPoZ-NDN7kJPozfz09PoPcZOBHwWIOovAFAWsjbqVKkzA3Uti-oVWwgwdQElmNds8R95zz6k9BtAiGVVv2NvtTagxYI9rXgXR-KJ-hRyuKdZNsEHhznEnkfP8y3xWWGLiTimhBN_CPl2v1-3ON7x-Dj9op5TSy6PsaGvfDUM7YtFjnv0LvSUg-Mpb5vpxXcg5C2h59T_nTo6Zm88tok-Ps8jdvPt4uf6srj-8f1qvbouSKgqF-gloNG10YQbROmlW5YbqWcJfqOM974p9ZLIoVRKSSeNJBICUENDVV0esU8H32GMf7aUsu1CctS22FPcJquXALqSagZPnsHtpqPGDmPocJzsIb35fH440_zsfaDRJheod9SEcc7CNjFYAXZXlt01YXdNWKXsvixblf8AguaItg</recordid><startdate>197706</startdate><enddate>197706</enddate><creator>del Río, Luís A.</creator><creator>Ortega, M.Gómez</creator><creator>López, A.Leal</creator><creator>Gorgé, J.López</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>197706</creationdate><title>A more sensitive modification of the catalase assay with the Clark oxygen electrode: Application to the kinetic study of the pea leaf enzyme</title><author>del Río, Luís A. ; Ortega, M.Gómez ; López, A.Leal ; Gorgé, J.López</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e175t-af20a98698eabaa2f2c43b288ea0fb79fffd384eeca27772c292ee110a80de563</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1977</creationdate><topic>Catalase - analysis</topic><topic>Electrodes</topic><topic>Evaluation Studies as Topic</topic><topic>Kinetics</topic><topic>Methods</topic><topic>Microchemistry</topic><topic>Oxygen</topic><topic>Plants - enzymology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>del Río, Luís A.</creatorcontrib><creatorcontrib>Ortega, M.Gómez</creatorcontrib><creatorcontrib>López, A.Leal</creatorcontrib><creatorcontrib>Gorgé, J.López</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>del Río, Luís A.</au><au>Ortega, M.Gómez</au><au>López, A.Leal</au><au>Gorgé, J.López</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A more sensitive modification of the catalase assay with the Clark oxygen electrode: Application to the kinetic study of the pea leaf enzyme</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>1977-06</date><risdate>1977</risdate><volume>80</volume><issue>2</issue><spage>409</spage><epage>415</epage><pages>409-415</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>A modification of the method of catalase determination by means of the Clark oxygen electrode is described. The assay is based on measurement of the initial rate at which oxygen is released by catalase in an oxygen-free buffer. Displacement of oxygen was brought about by flushing with nitrogen, and the substrate used was hydrogen peroxide at a 33.5 m
m final concentration. The method is rapid and can be used with crude catalase preparations. Its sensitivity is at least 20 times higher than that of previous methods; it has an interval of measurable activity of about 0.01–8.4 μmol of O
2/min and, therefore, is applicable to an 840-fold range of catalase concentrations. This modification was applied to the kinetic study of crude extracts of pea leaf catalase. An apparent
K
m
of 0.190
m was calculated.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>889081</pmid><doi>10.1016/0003-2697(77)90662-5</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Analytical biochemistry, 1977-06, Vol.80 (2), p.409-415 |
| issn | 0003-2697 1096-0309 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Catalase - analysis Electrodes Evaluation Studies as Topic Kinetics Methods Microchemistry Oxygen Plants - enzymology |
| title | A more sensitive modification of the catalase assay with the Clark oxygen electrode: Application to the kinetic study of the pea leaf enzyme |
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