Cytofluorograf Detection of Plasmodium yoelii, Trypanosoma gambiense, and Trypanosoma equiperdum by Laser Excited Fluorescence of Stained Rodent Blood

Samples of rat blood infected with Plasmodium yoelii (3% parasitized erythrocytes), Trypanosoma gambiense, or Trypanosoma equiperdum (> 50 trypanosomes per microscope field at 400 x) were fixed with 0.5% glutaraldehyde in phosphate buffered saline, then stained with acridine orange (AO) at 10-4,...

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