Fluorometric determination of acetaldehyde and its related compounds with o-phenylphenol
In the presence of nitrite ion, the reaction between acetaldehyde and o-phenylphenol in sulfuric acid was found to progress instantaneously at room temperature, causing an enhancement of fluorescence intensity. A simple and rapid microdetermination of acetaldehyde was thus established. Applications...
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| Veröffentlicht in: | Analytical biochemistry 1977-05, Vol.79 (1), p.73-82 |
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| container_end_page | 82 |
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| container_title | Analytical biochemistry |
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| creator | Asabe, Yoshihiro Kojima, Susumu Suzuki, Masao Takitani, Shoji |
| description | In the presence of nitrite ion, the reaction between acetaldehyde and
o-phenylphenol in sulfuric acid was found to progress instantaneously at room temperature, causing an enhancement of fluorescence intensity. A simple and rapid microdetermination of acetaldehyde was thus established. Applications of the proposed method were examined in order to quantitate lactic acid and muramic acid which easily liberate acetaldehyde when heated with sulfuric acid. By this method, 1–10 nmol of acetaldehyde, 1.1–22.5 nmol of lactic acid, and 2.1–23.2 nmol of muramic acid in a 200-μl sample can be determined with precision. Besides glyceraldehyde, α-hydroxyearboxylic acids, and aliphatic aldehydes, the other compounds tested did not interfere with the determination of lactic acid. Furthermore, except for amino sugars, there was no interference in the assay for muramic acid by naturally occurring alcohols, amines, amino acids, carboxylic acids, and sugars. The interference of amino sugars can be completely removed by treatment with ion-exchange resin. |
| doi_str_mv | 10.1016/0003-2697(77)90380-3 |
| format | Article |
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o-phenylphenol in sulfuric acid was found to progress instantaneously at room temperature, causing an enhancement of fluorescence intensity. A simple and rapid microdetermination of acetaldehyde was thus established. Applications of the proposed method were examined in order to quantitate lactic acid and muramic acid which easily liberate acetaldehyde when heated with sulfuric acid. By this method, 1–10 nmol of acetaldehyde, 1.1–22.5 nmol of lactic acid, and 2.1–23.2 nmol of muramic acid in a 200-μl sample can be determined with precision. Besides glyceraldehyde, α-hydroxyearboxylic acids, and aliphatic aldehydes, the other compounds tested did not interfere with the determination of lactic acid. Furthermore, except for amino sugars, there was no interference in the assay for muramic acid by naturally occurring alcohols, amines, amino acids, carboxylic acids, and sugars. The interference of amino sugars can be completely removed by treatment with ion-exchange resin.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>869206</pmid><doi>10.1016/0003-2697(77)90380-3</doi><tpages>10</tpages></addata></record> |
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| ispartof | Analytical biochemistry, 1977-05, Vol.79 (1), p.73-82 |
| issn | 0003-2697 1096-0309 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Acetaldehyde - analysis Lactates - analysis Muramic Acids - analysis Nitrates Phenols Spectrometry, Fluorescence Sulfuric Acids |
| title | Fluorometric determination of acetaldehyde and its related compounds with o-phenylphenol |
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