Cytological and cytochemical analysis of two mouse cancer cell lines. Caryotype, number of nucleoli, DNA, RNA and protein contents

Ehrlich ascites tumour cells (hyperdiploid strain ELD and hypertetraploid strain ELT) propagated in the mouse and TIII mammary carcinoma cells (strains TIII t and TIII r) cultivated in vitro are analysed with cytological and quantitative cytochemical methods. It is shown that, in these cancer cell lines, the number of nucleoli and the DNA, RNA and total protein contents are related to the number of chromosomes. These cellular parameters are also analysed during the cell cycle.