Zinc is associated with the β subunit of DNA-dependent RNA polymerase of Bacillus subtilis

The Bacillus subtilis DNA-dependent RNA polymerase holoenzyme and core enzyme each contain approximately two atoms of zinc per molecule. When the dissociated subunits of the enzyme are passed through a blue dextran-Sepharose affinity column, only the beta subunit binds to the column. The total zinc...

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Veröffentlicht in:	Biochemistry (Easton) 1977-06, Vol.16 (13), p.2880-2884
Hauptverfasser:	Halling, Shirley M, Sanchez-Anzaldo, F. J, Fukuda, Ryuji, Doi, Roy H, Meares, Claude F
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacillus subtilis - enzymology Chromatography, Affinity DNA-Directed RNA Polymerases - analysis DNA-Directed RNA Polymerases - antagonists & inhibitors DNA-Directed RNA Polymerases - metabolism Macromolecular Substances Metalloproteins - analysis Phenanthrolines - pharmacology Protein Binding Zinc - analysis
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