Zinc is associated with the β subunit of DNA-dependent RNA polymerase of Bacillus subtilis
The Bacillus subtilis DNA-dependent RNA polymerase holoenzyme and core enzyme each contain approximately two atoms of zinc per molecule. When the dissociated subunits of the enzyme are passed through a blue dextran-Sepharose affinity column, only the beta subunit binds to the column. The total zinc...
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| Veröffentlicht in: | Biochemistry (Easton) 1977-06, Vol.16 (13), p.2880-2884 |
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| container_issue | 13 |
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| container_title | Biochemistry (Easton) |
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| creator | Halling, Shirley M Sanchez-Anzaldo, F. J Fukuda, Ryuji Doi, Roy H Meares, Claude F |
| description | The Bacillus subtilis DNA-dependent RNA polymerase holoenzyme and core enzyme each contain approximately two atoms of zinc per molecule. When the dissociated subunits of the enzyme are passed through a blue dextran-Sepharose affinity column, only the beta subunit binds to the column. The total zinc content of the enzyme is tightly bound to the beta subunit. Dialysis studies suggest that the two zinc ions differ in the strength of their association with the beta subunit. The presence of zinc in beta is consistent with several other lines of evidence which indicate that this subunit is dirrectly involved in phosphodiester bond formation. The blue dextran-Sepharose column procedure should be useful in future studies of the dissociation and reassociation of the enzyme since the method is rapid and provides excellent recovery of the beta subunit as well as the alpha and beta' subunits of the RNA polymerase. |
| doi_str_mv | 10.1021/bi00632a012 |
| format | Article |
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The blue dextran-Sepharose column procedure should be useful in future studies of the dissociation and reassociation of the enzyme since the method is rapid and provides excellent recovery of the beta subunit as well as the alpha and beta' subunits of the RNA polymerase.</description><identifier>ISSN: 0006-2960</identifier><identifier>EISSN: 1520-4995</identifier><identifier>DOI: 10.1021/bi00632a012</identifier><identifier>PMID: 406910</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Bacillus subtilis - enzymology ; Chromatography, Affinity ; DNA-Directed RNA Polymerases - analysis ; DNA-Directed RNA Polymerases - antagonists & inhibitors ; DNA-Directed RNA Polymerases - metabolism ; Macromolecular Substances ; Metalloproteins - analysis ; Phenanthrolines - pharmacology ; Protein Binding ; Zinc - analysis</subject><ispartof>Biochemistry (Easton), 1977-06, Vol.16 (13), p.2880-2884</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a353t-ca9e287731cee61b581a5ed670fbd76a87a37ef3ae1098cda450435ff95452d53</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/bi00632a012$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/bi00632a012$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/406910$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Halling, Shirley M</creatorcontrib><creatorcontrib>Sanchez-Anzaldo, F. 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The blue dextran-Sepharose column procedure should be useful in future studies of the dissociation and reassociation of the enzyme since the method is rapid and provides excellent recovery of the beta subunit as well as the alpha and beta' subunits of the RNA polymerase.</description><subject>Bacillus subtilis - enzymology</subject><subject>Chromatography, Affinity</subject><subject>DNA-Directed RNA Polymerases - analysis</subject><subject>DNA-Directed RNA Polymerases - antagonists & inhibitors</subject><subject>DNA-Directed RNA Polymerases - metabolism</subject><subject>Macromolecular Substances</subject><subject>Metalloproteins - analysis</subject><subject>Phenanthrolines - pharmacology</subject><subject>Protein Binding</subject><subject>Zinc - analysis</subject><issn>0006-2960</issn><issn>1520-4995</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1977</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkMFu1DAQQC1UaJfCqdcefGoPKGDHcRwft0tbkEpBy8KBHqyJM1FdssmScQT7W3wI30RWWVUcOI2s9zwjPcZOpHgtRSrflEGIXKUgZPqEzaRORZJZqw_YTIwgSW0ujthzoofxmQmTHbJnmcitFDN29y20ngfiQNT5ABEr_jPEex7vkf_5zWkohzZE3tX87e08qXCDbYVt5MvbOd90zXaNPRDu-AX40DQD7f7E0AR6wZ7W0BC-3M9j9uXqcrV4l9x8vH6_mN8koLSKiQeLaWGMkh4xl6UuJGisciPqsjI5FAaUwVoBSmELX0GmRaZ0XVud6bTS6pidTXs3ffdjQIpuHchj00CL3UCuUGMMmxWj-GoSfd8R9Vi7TR_W0G-dFG5X0v1TcrRP92uHco3VozulG3Ey4UARfz1S6L-73Cij3erTZyf18uuVXVy7D6N_PvngyT10Q9-OTf57-C9c2InE</recordid><startdate>19770601</startdate><enddate>19770601</enddate><creator>Halling, Shirley M</creator><creator>Sanchez-Anzaldo, F. 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J</creatorcontrib><creatorcontrib>Fukuda, Ryuji</creatorcontrib><creatorcontrib>Doi, Roy H</creatorcontrib><creatorcontrib>Meares, Claude F</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemistry (Easton)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Halling, Shirley M</au><au>Sanchez-Anzaldo, F. 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The presence of zinc in beta is consistent with several other lines of evidence which indicate that this subunit is dirrectly involved in phosphodiester bond formation. The blue dextran-Sepharose column procedure should be useful in future studies of the dissociation and reassociation of the enzyme since the method is rapid and provides excellent recovery of the beta subunit as well as the alpha and beta' subunits of the RNA polymerase.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>406910</pmid><doi>10.1021/bi00632a012</doi><tpages>5</tpages></addata></record> |
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| identifier | ISSN: 0006-2960 |
| ispartof | Biochemistry (Easton), 1977-06, Vol.16 (13), p.2880-2884 |
| issn | 0006-2960 1520-4995 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_83995948 |
| source | American Chemical Society; MEDLINE |
| subjects | Bacillus subtilis - enzymology Chromatography, Affinity DNA-Directed RNA Polymerases - analysis DNA-Directed RNA Polymerases - antagonists & inhibitors DNA-Directed RNA Polymerases - metabolism Macromolecular Substances Metalloproteins - analysis Phenanthrolines - pharmacology Protein Binding Zinc - analysis |
| title | Zinc is associated with the β subunit of DNA-dependent RNA polymerase of Bacillus subtilis |
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