Visualization of peroxidase isozymes with eugenol, a noncarcinogenic substrate

Plant peroxidase isozymes utilize hydrogen peroxide to oxidize redox dyes. Benzidine, the substrate most commonly used for identification of peroxidase isozymes, is a potent carcinogen and has been banned from laboratory use. O-Dianisidine, the other common substrate for peroxidase isozymes, is stru...

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Veröffentlicht in:Analytical biochemistry 1977-05, Vol.79 (1), p.597-601
Hauptverfasser: Liu, Edwin H., Gibson, Donna M.
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Gibson, Donna M.
description Plant peroxidase isozymes utilize hydrogen peroxide to oxidize redox dyes. Benzidine, the substrate most commonly used for identification of peroxidase isozymes, is a potent carcinogen and has been banned from laboratory use. O-Dianisidine, the other common substrate for peroxidase isozymes, is structurally related to benzidine and is a possible carcinogen. A peroxidase zymogram stain has been developed which uses eugenol, a substrate which is safe to use in the laboratory. Peroxidase isozymes are recognized as bright blue fluorescent bands under ultraviolet light and develop within 1 min after staining. Eugenol may also be used in a quantitative fluorimetric assay of peroxidase activity.
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subjects Benzidines - metabolism
Eugenol - metabolism
Isoenzymes - analysis
Peroxidases - analysis
Plants - enzymology
Spectrometry, Fluorescence
title Visualization of peroxidase isozymes with eugenol, a noncarcinogenic substrate
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