Visualization of peroxidase isozymes with eugenol, a noncarcinogenic substrate
Plant peroxidase isozymes utilize hydrogen peroxide to oxidize redox dyes. Benzidine, the substrate most commonly used for identification of peroxidase isozymes, is a potent carcinogen and has been banned from laboratory use. O-Dianisidine, the other common substrate for peroxidase isozymes, is stru...
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Veröffentlicht in: | Analytical biochemistry 1977-05, Vol.79 (1), p.597-601 |
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creator | Liu, Edwin H. Gibson, Donna M. |
description | Plant peroxidase isozymes utilize hydrogen peroxide to oxidize redox dyes. Benzidine, the substrate most commonly used for identification of peroxidase isozymes, is a potent carcinogen and has been banned from laboratory use.
O-Dianisidine, the other common substrate for peroxidase isozymes, is structurally related to benzidine and is a possible carcinogen. A peroxidase zymogram stain has been developed which uses eugenol, a substrate which is safe to use in the laboratory. Peroxidase isozymes are recognized as bright blue fluorescent bands under ultraviolet light and develop within 1 min after staining. Eugenol may also be used in a quantitative fluorimetric assay of peroxidase activity. |
doi_str_mv | 10.1016/0003-2697(77)90439-0 |
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O-Dianisidine, the other common substrate for peroxidase isozymes, is structurally related to benzidine and is a possible carcinogen. A peroxidase zymogram stain has been developed which uses eugenol, a substrate which is safe to use in the laboratory. Peroxidase isozymes are recognized as bright blue fluorescent bands under ultraviolet light and develop within 1 min after staining. Eugenol may also be used in a quantitative fluorimetric assay of peroxidase activity.</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1016/0003-2697(77)90439-0</identifier><identifier>PMID: 869198</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Benzidines - metabolism ; Eugenol - metabolism ; Isoenzymes - analysis ; Peroxidases - analysis ; Plants - enzymology ; Spectrometry, Fluorescence</subject><ispartof>Analytical biochemistry, 1977-05, Vol.79 (1), p.597-601</ispartof><rights>1977</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c271t-1f559bed5e32e89e720dd867898cb914c78b31a06e6e68c54f5b2fb625d6fdd73</citedby><cites>FETCH-LOGICAL-c271t-1f559bed5e32e89e720dd867898cb914c78b31a06e6e68c54f5b2fb625d6fdd73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0003269777904390$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/869198$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Edwin H.</creatorcontrib><creatorcontrib>Gibson, Donna M.</creatorcontrib><title>Visualization of peroxidase isozymes with eugenol, a noncarcinogenic substrate</title><title>Analytical biochemistry</title><addtitle>Anal Biochem</addtitle><description>Plant peroxidase isozymes utilize hydrogen peroxide to oxidize redox dyes. Benzidine, the substrate most commonly used for identification of peroxidase isozymes, is a potent carcinogen and has been banned from laboratory use.
O-Dianisidine, the other common substrate for peroxidase isozymes, is structurally related to benzidine and is a possible carcinogen. A peroxidase zymogram stain has been developed which uses eugenol, a substrate which is safe to use in the laboratory. Peroxidase isozymes are recognized as bright blue fluorescent bands under ultraviolet light and develop within 1 min after staining. Eugenol may also be used in a quantitative fluorimetric assay of peroxidase activity.</description><subject>Benzidines - metabolism</subject><subject>Eugenol - metabolism</subject><subject>Isoenzymes - analysis</subject><subject>Peroxidases - analysis</subject><subject>Plants - enzymology</subject><subject>Spectrometry, Fluorescence</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1977</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtLxDAQx4P4Wle_wR56EgWrSdPmcRFk8QWLXtRrSJOpRrrNmrTq7qe3axePMoeB-T9gfghNCD4nmLALjDFNMyb5CeenEudUpngLjQiWLMUUy200-rPso4MY3zEmJC_YHtoVTBIpRujhxcVO126lW-ebxFfJAoL_dlZHSFz0q-UcYvLl2rcEuldofH2W6KTxjdHBuMb3J2eS2JWxDbqFQ7RT6TrC0WaP0fPN9dP0Lp093t5Pr2apyThpU1IVhSzBFkAzEBJ4hq0VjAspTClJbrgoKdGYQT_CFHlVlFlVsqywrLKW0zE6HnoXwX90EFs1d9FAXesGfBeVoFIUlInemA9GE3yMASq1CG6uw1IRrNYU1RqRWiNSnKtfigr3scmmvyvnYP9CA7Zevhxk6H_8dBBUNA4aA9YFMK2y3v3f_wPN4IJp</recordid><startdate>19770501</startdate><enddate>19770501</enddate><creator>Liu, Edwin H.</creator><creator>Gibson, Donna M.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19770501</creationdate><title>Visualization of peroxidase isozymes with eugenol, a noncarcinogenic substrate</title><author>Liu, Edwin H. ; Gibson, Donna M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c271t-1f559bed5e32e89e720dd867898cb914c78b31a06e6e68c54f5b2fb625d6fdd73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1977</creationdate><topic>Benzidines - metabolism</topic><topic>Eugenol - metabolism</topic><topic>Isoenzymes - analysis</topic><topic>Peroxidases - analysis</topic><topic>Plants - enzymology</topic><topic>Spectrometry, Fluorescence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Edwin H.</creatorcontrib><creatorcontrib>Gibson, Donna M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Edwin H.</au><au>Gibson, Donna M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Visualization of peroxidase isozymes with eugenol, a noncarcinogenic substrate</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>1977-05-01</date><risdate>1977</risdate><volume>79</volume><issue>1</issue><spage>597</spage><epage>601</epage><pages>597-601</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>Plant peroxidase isozymes utilize hydrogen peroxide to oxidize redox dyes. Benzidine, the substrate most commonly used for identification of peroxidase isozymes, is a potent carcinogen and has been banned from laboratory use.
O-Dianisidine, the other common substrate for peroxidase isozymes, is structurally related to benzidine and is a possible carcinogen. A peroxidase zymogram stain has been developed which uses eugenol, a substrate which is safe to use in the laboratory. Peroxidase isozymes are recognized as bright blue fluorescent bands under ultraviolet light and develop within 1 min after staining. Eugenol may also be used in a quantitative fluorimetric assay of peroxidase activity.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>869198</pmid><doi>10.1016/0003-2697(77)90439-0</doi><tpages>5</tpages></addata></record> |
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subjects | Benzidines - metabolism Eugenol - metabolism Isoenzymes - analysis Peroxidases - analysis Plants - enzymology Spectrometry, Fluorescence |
title | Visualization of peroxidase isozymes with eugenol, a noncarcinogenic substrate |
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