Study of DNA synthesis in chromatin isolated from HeLa cells
When incubated in vitro, HeLa cell chromatin can synthesize DNA at rate comparable to that observed with isolated nuclei. The in vitro DNA synthetic activity of chromatin reflects DNA synthesis in intact cells since chromatin from cells in S phase are several times more active thatn preparations der...
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| Veröffentlicht in: | Biochemistry (Easton) 1977-07, Vol.16 (14), p.3190-3194 |
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| container_title | Biochemistry (Easton) |
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| creator | Knopf, Karl Werner Weissbach, Arthur |
| description | When incubated in vitro, HeLa cell chromatin can synthesize DNA at rate comparable to that observed with isolated nuclei. The in vitro DNA synthetic activity of chromatin reflects DNA synthesis in intact cells since chromatin from cells in S phase are several times more active thatn preparations derived from mitotic cells. The requirements for the synthesis of DNA by chromatin preparations are also similar to those of isolated nuclei and the size of the DNA pieces made in both systems is roughly comparable. The chromatin system offers several advantages not available with isolated nuclei. Chromatin will synthesize DNA for a much longer time than isolated nuclei so that larger amounts of DNA can be synthesized in vitro In addition, although chromatin has its own endogenous ability to synthesize DNA, it is markedly stimulated by the presence of exogenously added HeLa cell DNA polymerase alpha, beta, and gamma, and, thus, may provide a new template system for the study of DNA synthesis. |
| doi_str_mv | 10.1021/bi00633a023 |
| format | Article |
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The in vitro DNA synthetic activity of chromatin reflects DNA synthesis in intact cells since chromatin from cells in S phase are several times more active thatn preparations derived from mitotic cells. The requirements for the synthesis of DNA by chromatin preparations are also similar to those of isolated nuclei and the size of the DNA pieces made in both systems is roughly comparable. The chromatin system offers several advantages not available with isolated nuclei. Chromatin will synthesize DNA for a much longer time than isolated nuclei so that larger amounts of DNA can be synthesized in vitro In addition, although chromatin has its own endogenous ability to synthesize DNA, it is markedly stimulated by the presence of exogenously added HeLa cell DNA polymerase alpha, beta, and gamma, and, thus, may provide a new template system for the study of DNA synthesis.</description><identifier>ISSN: 0006-2960</identifier><identifier>EISSN: 1520-4995</identifier><identifier>DOI: 10.1021/bi00633a023</identifier><identifier>PMID: 889795</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Cell Nucleus - metabolism ; Cell-Free System ; Chromatin - metabolism ; DNA, Neoplasm - biosynthesis ; DNA-Directed DNA Polymerase - isolation & purification ; HeLa Cells - metabolism ; Humans ; Kinetics ; Mitosis ; RNA, Neoplasm - biosynthesis</subject><ispartof>Biochemistry (Easton), 1977-07, Vol.16 (14), p.3190-3194</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a353t-371cbb6ef955807d40a043b00afd66bc536682e0da5513e00e09a4b5b846bfc83</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/bi00633a023$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/bi00633a023$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/889795$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Knopf, Karl Werner</creatorcontrib><creatorcontrib>Weissbach, Arthur</creatorcontrib><title>Study of DNA synthesis in chromatin isolated from HeLa cells</title><title>Biochemistry (Easton)</title><addtitle>Biochemistry</addtitle><description>When incubated in vitro, HeLa cell chromatin can synthesize DNA at rate comparable to that observed with isolated nuclei. The in vitro DNA synthetic activity of chromatin reflects DNA synthesis in intact cells since chromatin from cells in S phase are several times more active thatn preparations derived from mitotic cells. The requirements for the synthesis of DNA by chromatin preparations are also similar to those of isolated nuclei and the size of the DNA pieces made in both systems is roughly comparable. The chromatin system offers several advantages not available with isolated nuclei. Chromatin will synthesize DNA for a much longer time than isolated nuclei so that larger amounts of DNA can be synthesized in vitro In addition, although chromatin has its own endogenous ability to synthesize DNA, it is markedly stimulated by the presence of exogenously added HeLa cell DNA polymerase alpha, beta, and gamma, and, thus, may provide a new template system for the study of DNA synthesis.</description><subject>Cell Nucleus - metabolism</subject><subject>Cell-Free System</subject><subject>Chromatin - metabolism</subject><subject>DNA, Neoplasm - biosynthesis</subject><subject>DNA-Directed DNA Polymerase - isolation & purification</subject><subject>HeLa Cells - metabolism</subject><subject>Humans</subject><subject>Kinetics</subject><subject>Mitosis</subject><subject>RNA, Neoplasm - biosynthesis</subject><issn>0006-2960</issn><issn>1520-4995</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1977</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkDtPwzAUhS3EqxQmVgZPMKDAdRw7scRSFWiQKkBqmS07cdSUPMBOJPrvcZWqYmC6j_Pp3KuD0CWBOwIhudclAKdUQUgP0IiwEIJICHaIRuCFIBQcTtGZc2s_RhBHJ-g4SUQs2Ag9LLo-3-C2wI-vE-w2TbcyrnS4bHC2sm2tOt-Vrq1UZ3Jc-A1OzVzhzFSVO0dHhaqcudjVMfp4flpO02D-NnuZTuaBoox2AY1JpjU3hWAsgTiPQEFENYAqcs51xijnSWggV4wRagAMCBVpppOI6yJL6BhdD75ftv3ujetkXbrtB6oxbe9kQkXMGBcevB3AzLbOWVPIL1vWym4kAbmNSv6JytNXO9te1ybfs0M2Xg4GuXSd-dmryn5KHtOYyeX7Qs5SHhLKUxl6_mbgVebkuu1t4zP59_AvYfJ9Nw</recordid><startdate>19770712</startdate><enddate>19770712</enddate><creator>Knopf, Karl Werner</creator><creator>Weissbach, Arthur</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19770712</creationdate><title>Study of DNA synthesis in chromatin isolated from HeLa cells</title><author>Knopf, Karl Werner ; Weissbach, Arthur</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a353t-371cbb6ef955807d40a043b00afd66bc536682e0da5513e00e09a4b5b846bfc83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1977</creationdate><topic>Cell Nucleus - metabolism</topic><topic>Cell-Free System</topic><topic>Chromatin - metabolism</topic><topic>DNA, Neoplasm - biosynthesis</topic><topic>DNA-Directed DNA Polymerase - isolation & purification</topic><topic>HeLa Cells - metabolism</topic><topic>Humans</topic><topic>Kinetics</topic><topic>Mitosis</topic><topic>RNA, Neoplasm - biosynthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Knopf, Karl Werner</creatorcontrib><creatorcontrib>Weissbach, Arthur</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemistry (Easton)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Knopf, Karl Werner</au><au>Weissbach, Arthur</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Study of DNA synthesis in chromatin isolated from HeLa cells</atitle><jtitle>Biochemistry (Easton)</jtitle><addtitle>Biochemistry</addtitle><date>1977-07-12</date><risdate>1977</risdate><volume>16</volume><issue>14</issue><spage>3190</spage><epage>3194</epage><pages>3190-3194</pages><issn>0006-2960</issn><eissn>1520-4995</eissn><abstract>When incubated in vitro, HeLa cell chromatin can synthesize DNA at rate comparable to that observed with isolated nuclei. The in vitro DNA synthetic activity of chromatin reflects DNA synthesis in intact cells since chromatin from cells in S phase are several times more active thatn preparations derived from mitotic cells. The requirements for the synthesis of DNA by chromatin preparations are also similar to those of isolated nuclei and the size of the DNA pieces made in both systems is roughly comparable. The chromatin system offers several advantages not available with isolated nuclei. Chromatin will synthesize DNA for a much longer time than isolated nuclei so that larger amounts of DNA can be synthesized in vitro In addition, although chromatin has its own endogenous ability to synthesize DNA, it is markedly stimulated by the presence of exogenously added HeLa cell DNA polymerase alpha, beta, and gamma, and, thus, may provide a new template system for the study of DNA synthesis.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>889795</pmid><doi>10.1021/bi00633a023</doi><tpages>5</tpages></addata></record> |
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| issn | 0006-2960 1520-4995 |
| language | eng |
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| source | ACS Publications; MEDLINE |
| subjects | Cell Nucleus - metabolism Cell-Free System Chromatin - metabolism DNA, Neoplasm - biosynthesis DNA-Directed DNA Polymerase - isolation & purification HeLa Cells - metabolism Humans Kinetics Mitosis RNA, Neoplasm - biosynthesis |
| title | Study of DNA synthesis in chromatin isolated from HeLa cells |
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