Plasma Thromboplastin Antecedent (PTA, Factor XI): A Specific and Sensitive Radioimmunoassay
A specific, sensitive, and reproducible radioimmunoassay for human plasma thromboplastin antecedent (PTA, factor XI) has been developed with purified PTA and monospecific rabbit antiserum. Precise measurements of PTA antigen were possible for concentrations as low as 0.3% of that in normal pooled pl...
Gespeichert in:
| Veröffentlicht in: | Blood 1977-09, Vol.50 (3), p.377,383-381,385 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 381,385 |
|---|---|
| container_issue | 3 |
| container_start_page | 377,383 |
| container_title | Blood |
| container_volume | 50 |
| creator | Saito, Hidehiko Goldsmith, George H. |
| description | A specific, sensitive, and reproducible radioimmunoassay for human plasma thromboplastin antecedent (PTA, factor XI) has been developed with purified PTA and monospecific rabbit antiserum. Precise measurements of PTA antigen were possible for concentrations as low as 0.3% of that in normal pooled plasma. Normal plasma contained approximately 6 μg PTA/ml. A good correlation (correlation coefficient 0.68) existed between the PTA procoagulant assays and radioimmunoassays among 50 normal adults (25 males and 25 females). PTA antigen was markedly reduced in plasma of 13 patients with congenital homozygous PTA deficiency (range < 0.003-0.128 U/ml) and 9 patients with hepatic cirrhosis (0.35 ± 0.17 U/ml), but was normal in those of 9 patients under treatment with warfarin, 8 patients with disseminated intravascular coagulation and 16 patients with other congenital clotting factor abnormalities, including prekallikrein deficiency (Fletcher trait) and high molecular weight kininogen deficiency (Fitzgerald trait). |
| doi_str_mv | 10.1182/blood.V50.3.377.377 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_83975081</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0006497120727722</els_id><sourcerecordid>83975081</sourcerecordid><originalsourceid>FETCH-LOGICAL-c330t-f6839b908a0277888ea4349b14e7480cea846b2fa336681275c74daf67d5876c3</originalsourceid><addsrcrecordid>eNp9kEtLAzEQx4P4qtVPoIecRMGtySa7SQUPRXwUChZbxYMQstlZjOxuarIt-O1NrXj0MAzD_wHzQ-iYkgGlMr0saufKwUtGBmzAhFjPFurRLJUJISnZRj1CSJ7woaD76CCED0IoZ2m2h3al5IzmPfQ2rXVoNJ6_e9cUbhGvzrZ41HZgoIS2w2fT-egC32nTOY9fx-dXeIRnCzC2sgbrtsQzaIPt7Arwky6ts02zbJ0OQX8dop1K1wGOfncfPd_dzm8eksnj_fhmNEkMY6RLqlyyYTEkUpNUCCklaM74sKAcBJfEgJY8L9JKM5bnkqYiM4KXuspFmUmRG9ZHp5vehXefSwidamwwUNe6BbcMKtaLjEgajWxjNN6F4KFSC28b7b8UJWqNVP0gVRGpYiriXE9MnfzWL4sGyr_MhmGUrzcyxBdXFrwKxkIb8VkPplOls__WfwNZYob3</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>83975081</pqid></control><display><type>article</type><title>Plasma Thromboplastin Antecedent (PTA, Factor XI): A Specific and Sensitive Radioimmunoassay</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Saito, Hidehiko ; Goldsmith, George H.</creator><creatorcontrib>Saito, Hidehiko ; Goldsmith, George H.</creatorcontrib><description>A specific, sensitive, and reproducible radioimmunoassay for human plasma thromboplastin antecedent (PTA, factor XI) has been developed with purified PTA and monospecific rabbit antiserum. Precise measurements of PTA antigen were possible for concentrations as low as 0.3% of that in normal pooled plasma. Normal plasma contained approximately 6 μg PTA/ml. A good correlation (correlation coefficient 0.68) existed between the PTA procoagulant assays and radioimmunoassays among 50 normal adults (25 males and 25 females). PTA antigen was markedly reduced in plasma of 13 patients with congenital homozygous PTA deficiency (range < 0.003-0.128 U/ml) and 9 patients with hepatic cirrhosis (0.35 ± 0.17 U/ml), but was normal in those of 9 patients under treatment with warfarin, 8 patients with disseminated intravascular coagulation and 16 patients with other congenital clotting factor abnormalities, including prekallikrein deficiency (Fletcher trait) and high molecular weight kininogen deficiency (Fitzgerald trait).</description><identifier>ISSN: 0006-4971</identifier><identifier>EISSN: 1528-0020</identifier><identifier>DOI: 10.1182/blood.V50.3.377.377</identifier><identifier>PMID: 884316</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adult ; Antibody Specificity ; Blood Coagulation Tests ; Disseminated Intravascular Coagulation - blood ; Factor XI - analysis ; Factor XI Deficiency - blood ; Female ; Humans ; Immune Sera ; Kininogens ; Liver Cirrhosis - blood ; Male ; Middle Aged ; Prekallikrein - deficiency ; Radioimmunoassay - methods ; Warfarin - therapeutic use</subject><ispartof>Blood, 1977-09, Vol.50 (3), p.377,383-381,385</ispartof><rights>1977 American Society of Hematology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c330t-f6839b908a0277888ea4349b14e7480cea846b2fa336681275c74daf67d5876c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/884316$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Saito, Hidehiko</creatorcontrib><creatorcontrib>Goldsmith, George H.</creatorcontrib><title>Plasma Thromboplastin Antecedent (PTA, Factor XI): A Specific and Sensitive Radioimmunoassay</title><title>Blood</title><addtitle>Blood</addtitle><description>A specific, sensitive, and reproducible radioimmunoassay for human plasma thromboplastin antecedent (PTA, factor XI) has been developed with purified PTA and monospecific rabbit antiserum. Precise measurements of PTA antigen were possible for concentrations as low as 0.3% of that in normal pooled plasma. Normal plasma contained approximately 6 μg PTA/ml. A good correlation (correlation coefficient 0.68) existed between the PTA procoagulant assays and radioimmunoassays among 50 normal adults (25 males and 25 females). PTA antigen was markedly reduced in plasma of 13 patients with congenital homozygous PTA deficiency (range < 0.003-0.128 U/ml) and 9 patients with hepatic cirrhosis (0.35 ± 0.17 U/ml), but was normal in those of 9 patients under treatment with warfarin, 8 patients with disseminated intravascular coagulation and 16 patients with other congenital clotting factor abnormalities, including prekallikrein deficiency (Fletcher trait) and high molecular weight kininogen deficiency (Fitzgerald trait).</description><subject>Adult</subject><subject>Antibody Specificity</subject><subject>Blood Coagulation Tests</subject><subject>Disseminated Intravascular Coagulation - blood</subject><subject>Factor XI - analysis</subject><subject>Factor XI Deficiency - blood</subject><subject>Female</subject><subject>Humans</subject><subject>Immune Sera</subject><subject>Kininogens</subject><subject>Liver Cirrhosis - blood</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Prekallikrein - deficiency</subject><subject>Radioimmunoassay - methods</subject><subject>Warfarin - therapeutic use</subject><issn>0006-4971</issn><issn>1528-0020</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1977</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtLAzEQx4P4qtVPoIecRMGtySa7SQUPRXwUChZbxYMQstlZjOxuarIt-O1NrXj0MAzD_wHzQ-iYkgGlMr0saufKwUtGBmzAhFjPFurRLJUJISnZRj1CSJ7woaD76CCED0IoZ2m2h3al5IzmPfQ2rXVoNJ6_e9cUbhGvzrZ41HZgoIS2w2fT-egC32nTOY9fx-dXeIRnCzC2sgbrtsQzaIPt7Arwky6ts02zbJ0OQX8dop1K1wGOfncfPd_dzm8eksnj_fhmNEkMY6RLqlyyYTEkUpNUCCklaM74sKAcBJfEgJY8L9JKM5bnkqYiM4KXuspFmUmRG9ZHp5vehXefSwidamwwUNe6BbcMKtaLjEgajWxjNN6F4KFSC28b7b8UJWqNVP0gVRGpYiriXE9MnfzWL4sGyr_MhmGUrzcyxBdXFrwKxkIb8VkPplOls__WfwNZYob3</recordid><startdate>197709</startdate><enddate>197709</enddate><creator>Saito, Hidehiko</creator><creator>Goldsmith, George H.</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197709</creationdate><title>Plasma Thromboplastin Antecedent (PTA, Factor XI): A Specific and Sensitive Radioimmunoassay</title><author>Saito, Hidehiko ; Goldsmith, George H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c330t-f6839b908a0277888ea4349b14e7480cea846b2fa336681275c74daf67d5876c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1977</creationdate><topic>Adult</topic><topic>Antibody Specificity</topic><topic>Blood Coagulation Tests</topic><topic>Disseminated Intravascular Coagulation - blood</topic><topic>Factor XI - analysis</topic><topic>Factor XI Deficiency - blood</topic><topic>Female</topic><topic>Humans</topic><topic>Immune Sera</topic><topic>Kininogens</topic><topic>Liver Cirrhosis - blood</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Prekallikrein - deficiency</topic><topic>Radioimmunoassay - methods</topic><topic>Warfarin - therapeutic use</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Saito, Hidehiko</creatorcontrib><creatorcontrib>Goldsmith, George H.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Blood</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Saito, Hidehiko</au><au>Goldsmith, George H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Plasma Thromboplastin Antecedent (PTA, Factor XI): A Specific and Sensitive Radioimmunoassay</atitle><jtitle>Blood</jtitle><addtitle>Blood</addtitle><date>1977-09</date><risdate>1977</risdate><volume>50</volume><issue>3</issue><spage>377,383</spage><epage>381,385</epage><pages>377,383-381,385</pages><issn>0006-4971</issn><eissn>1528-0020</eissn><abstract>A specific, sensitive, and reproducible radioimmunoassay for human plasma thromboplastin antecedent (PTA, factor XI) has been developed with purified PTA and monospecific rabbit antiserum. Precise measurements of PTA antigen were possible for concentrations as low as 0.3% of that in normal pooled plasma. Normal plasma contained approximately 6 μg PTA/ml. A good correlation (correlation coefficient 0.68) existed between the PTA procoagulant assays and radioimmunoassays among 50 normal adults (25 males and 25 females). PTA antigen was markedly reduced in plasma of 13 patients with congenital homozygous PTA deficiency (range < 0.003-0.128 U/ml) and 9 patients with hepatic cirrhosis (0.35 ± 0.17 U/ml), but was normal in those of 9 patients under treatment with warfarin, 8 patients with disseminated intravascular coagulation and 16 patients with other congenital clotting factor abnormalities, including prekallikrein deficiency (Fletcher trait) and high molecular weight kininogen deficiency (Fitzgerald trait).</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>884316</pmid><doi>10.1182/blood.V50.3.377.377</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-4971 |
| ispartof | Blood, 1977-09, Vol.50 (3), p.377,383-381,385 |
| issn | 0006-4971 1528-0020 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_83975081 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Adult Antibody Specificity Blood Coagulation Tests Disseminated Intravascular Coagulation - blood Factor XI - analysis Factor XI Deficiency - blood Female Humans Immune Sera Kininogens Liver Cirrhosis - blood Male Middle Aged Prekallikrein - deficiency Radioimmunoassay - methods Warfarin - therapeutic use |
| title | Plasma Thromboplastin Antecedent (PTA, Factor XI): A Specific and Sensitive Radioimmunoassay |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-27T16%3A25%3A56IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Plasma%20Thromboplastin%20Antecedent%20(PTA,%20Factor%20XI):%20A%20Specific%20and%20Sensitive%20Radioimmunoassay&rft.jtitle=Blood&rft.au=Saito,%20Hidehiko&rft.date=1977-09&rft.volume=50&rft.issue=3&rft.spage=377,383&rft.epage=381,385&rft.pages=377,383-381,385&rft.issn=0006-4971&rft.eissn=1528-0020&rft_id=info:doi/10.1182/blood.V50.3.377.377&rft_dat=%3Cproquest_cross%3E83975081%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=83975081&rft_id=info:pmid/884316&rft_els_id=S0006497120727722&rfr_iscdi=true |