Methanoregula boonei gen. nov., sp. nov., an acidiphilic methanogen isolated from an acidic peat bog
A novel acidiphilic, hydrogenotrophic methanogen, designated strain 6A8(T), was isolated from an acidic (pH 4.0-4.5) and ombrotrophic (rain-fed) bog located near Ithaca, NY, USA. Cultures were dimorphic, containing thin rods (0.2-0.3 μm in diameter and 0.8-3.0 μm long) and irregular cocci (0.2-0.8 μ...
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| Veröffentlicht in: | International journal of systematic and evolutionary microbiology 2011, Vol.61 (Pt 1), p.45-52 |
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| creator | BRÄUER, Suzanna L CADILLO-QUIROZ, Hinsby WARD, Rebekah J YAVITT, Joseph B ZINDER, Stephen H |
| description | A novel acidiphilic, hydrogenotrophic methanogen, designated strain 6A8(T), was isolated from an acidic (pH 4.0-4.5) and ombrotrophic (rain-fed) bog located near Ithaca, NY, USA. Cultures were dimorphic, containing thin rods (0.2-0.3 μm in diameter and 0.8-3.0 μm long) and irregular cocci (0.2-0.8 μm in diameter). The culture utilized H(2)/CO(2) to produce methane but did not utilize formate, acetate, methanol, ethanol, 2-propanol, butanol or trimethylamine. Optimal growth conditions were near pH 5.1 and 35 °C. The culture grew in basal medium containing as little as 0.43 mM Na(+) and growth was inhibited completely by 50 mM NaCl. To our knowledge, strain 6A8(T) is one of the most acidiphilic (lowest pH optimum) and salt-sensitive methanogens in pure culture. Acetate, coenzyme M, vitamins and yeast extract were required for growth. It is proposed that a new genus and species be established for this organism, Methanoregula boonei gen. nov., sp. nov. The type strain of Methanoregula boonei is 6A8(T) (=DSM 21154(T) =JCM 14090(T)). |
| doi_str_mv | 10.1099/ijs.0.021782-0 |
| format | Article |
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Cultures were dimorphic, containing thin rods (0.2-0.3 μm in diameter and 0.8-3.0 μm long) and irregular cocci (0.2-0.8 μm in diameter). The culture utilized H(2)/CO(2) to produce methane but did not utilize formate, acetate, methanol, ethanol, 2-propanol, butanol or trimethylamine. Optimal growth conditions were near pH 5.1 and 35 °C. The culture grew in basal medium containing as little as 0.43 mM Na(+) and growth was inhibited completely by 50 mM NaCl. To our knowledge, strain 6A8(T) is one of the most acidiphilic (lowest pH optimum) and salt-sensitive methanogens in pure culture. Acetate, coenzyme M, vitamins and yeast extract were required for growth. It is proposed that a new genus and species be established for this organism, Methanoregula boonei gen. nov., sp. nov. The type strain of Methanoregula boonei is 6A8(T) (=DSM 21154(T) =JCM 14090(T)).</description><identifier>ISSN: 1466-5026</identifier><identifier>EISSN: 1466-5034</identifier><identifier>DOI: 10.1099/ijs.0.021782-0</identifier><identifier>PMID: 20154331</identifier><language>eng</language><publisher>Reading: Society for General Microbiology</publisher><subject>Bacteriology ; Biological and medical sciences ; Carbon Dioxide - metabolism ; Cluster Analysis ; Culture Media - chemistry ; DNA, Archaeal - chemistry ; DNA, Archaeal - genetics ; DNA, Ribosomal - chemistry ; DNA, Ribosomal - genetics ; Environmental Microbiology ; Fundamental and applied biological sciences. Psychology ; Hydrogen - metabolism ; Hydrogen-Ion Concentration ; Methane - metabolism ; Methanomicrobiales - classification ; Methanomicrobiales - genetics ; Methanomicrobiales - isolation & purification ; Methanomicrobiales - physiology ; Microbiology ; Molecular Sequence Data ; Phylogeny ; RNA, Ribosomal, 16S - genetics ; Sequence Analysis, DNA ; Sodium Chloride - metabolism ; Soil ; Systematics ; Temperature ; United States ; Wetlands</subject><ispartof>International journal of systematic and evolutionary microbiology, 2011, Vol.61 (Pt 1), p.45-52</ispartof><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c475t-c06c47ae4d7780057d501da0b06d907be5a2ba1d5957d9ae6bc6647334ea1f903</citedby><cites>FETCH-LOGICAL-c475t-c06c47ae4d7780057d501da0b06d907be5a2ba1d5957d9ae6bc6647334ea1f903</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3733,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23905940$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20154331$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>BRÄUER, Suzanna L</creatorcontrib><creatorcontrib>CADILLO-QUIROZ, Hinsby</creatorcontrib><creatorcontrib>WARD, Rebekah J</creatorcontrib><creatorcontrib>YAVITT, Joseph B</creatorcontrib><creatorcontrib>ZINDER, Stephen H</creatorcontrib><title>Methanoregula boonei gen. nov., sp. nov., an acidiphilic methanogen isolated from an acidic peat bog</title><title>International journal of systematic and evolutionary microbiology</title><addtitle>Int J Syst Evol Microbiol</addtitle><description>A novel acidiphilic, hydrogenotrophic methanogen, designated strain 6A8(T), was isolated from an acidic (pH 4.0-4.5) and ombrotrophic (rain-fed) bog located near Ithaca, NY, USA. Cultures were dimorphic, containing thin rods (0.2-0.3 μm in diameter and 0.8-3.0 μm long) and irregular cocci (0.2-0.8 μm in diameter). The culture utilized H(2)/CO(2) to produce methane but did not utilize formate, acetate, methanol, ethanol, 2-propanol, butanol or trimethylamine. Optimal growth conditions were near pH 5.1 and 35 °C. The culture grew in basal medium containing as little as 0.43 mM Na(+) and growth was inhibited completely by 50 mM NaCl. To our knowledge, strain 6A8(T) is one of the most acidiphilic (lowest pH optimum) and salt-sensitive methanogens in pure culture. Acetate, coenzyme M, vitamins and yeast extract were required for growth. It is proposed that a new genus and species be established for this organism, Methanoregula boonei gen. nov., sp. nov. The type strain of Methanoregula boonei is 6A8(T) (=DSM 21154(T) =JCM 14090(T)).</description><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Carbon Dioxide - metabolism</subject><subject>Cluster Analysis</subject><subject>Culture Media - chemistry</subject><subject>DNA, Archaeal - chemistry</subject><subject>DNA, Archaeal - genetics</subject><subject>DNA, Ribosomal - chemistry</subject><subject>DNA, Ribosomal - genetics</subject><subject>Environmental Microbiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydrogen - metabolism</subject><subject>Hydrogen-Ion Concentration</subject><subject>Methane - metabolism</subject><subject>Methanomicrobiales - classification</subject><subject>Methanomicrobiales - genetics</subject><subject>Methanomicrobiales - isolation & purification</subject><subject>Methanomicrobiales - physiology</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Phylogeny</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>Sodium Chloride - metabolism</subject><subject>Soil</subject><subject>Systematics</subject><subject>Temperature</subject><subject>United States</subject><subject>Wetlands</subject><issn>1466-5026</issn><issn>1466-5034</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkD1PwzAQhi0EoqWwMiIviIWEcxwn9YgqvqQiFpiti-20rpI4xCkS_55Uact0r-6ee4aXkGsGMQMpH9wmxBBDwvJ5EsEJmbI0yyIBPD095iSbkIsQNgDDAuCcTBJgIuWcTYl5t_0aG9_Z1bZCWnjfWEdXtolp43_iexraQ8KGonbGtWtXOU3r8XFAqQu-wt4aWna-PnKathb7Qbm6JGclVsFe7eeMfD0_fS5eo-XHy9vicRnpNBd9pCEbAtrU5PkcQORGADMIBWRGQl5YgUmBzAg5nCTarNBZluacpxZZKYHPyN3obTv_vbWhV7UL2lYVNtZvg5pzmTORyGQg45HUnQ-hs6VqO1dj96sYqF2xaihWgRqLVTv1zV69LWprjvihyQG43QMYNFZlh4124Z_jEoRMgf8B-ECAJA</recordid><startdate>2011</startdate><enddate>2011</enddate><creator>BRÄUER, Suzanna L</creator><creator>CADILLO-QUIROZ, Hinsby</creator><creator>WARD, Rebekah J</creator><creator>YAVITT, Joseph B</creator><creator>ZINDER, Stephen H</creator><general>Society for General Microbiology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2011</creationdate><title>Methanoregula boonei gen. nov., sp. nov., an acidiphilic methanogen isolated from an acidic peat bog</title><author>BRÄUER, Suzanna L ; CADILLO-QUIROZ, Hinsby ; WARD, Rebekah J ; YAVITT, Joseph B ; ZINDER, Stephen H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c475t-c06c47ae4d7780057d501da0b06d907be5a2ba1d5957d9ae6bc6647334ea1f903</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Carbon Dioxide - metabolism</topic><topic>Cluster Analysis</topic><topic>Culture Media - chemistry</topic><topic>DNA, Archaeal - chemistry</topic><topic>DNA, Archaeal - genetics</topic><topic>DNA, Ribosomal - chemistry</topic><topic>DNA, Ribosomal - genetics</topic><topic>Environmental Microbiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hydrogen - metabolism</topic><topic>Hydrogen-Ion Concentration</topic><topic>Methane - metabolism</topic><topic>Methanomicrobiales - classification</topic><topic>Methanomicrobiales - genetics</topic><topic>Methanomicrobiales - isolation & purification</topic><topic>Methanomicrobiales - physiology</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Phylogeny</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Sequence Analysis, DNA</topic><topic>Sodium Chloride - metabolism</topic><topic>Soil</topic><topic>Systematics</topic><topic>Temperature</topic><topic>United States</topic><topic>Wetlands</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>BRÄUER, Suzanna L</creatorcontrib><creatorcontrib>CADILLO-QUIROZ, Hinsby</creatorcontrib><creatorcontrib>WARD, Rebekah J</creatorcontrib><creatorcontrib>YAVITT, Joseph B</creatorcontrib><creatorcontrib>ZINDER, Stephen H</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of systematic and evolutionary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>BRÄUER, Suzanna L</au><au>CADILLO-QUIROZ, Hinsby</au><au>WARD, Rebekah J</au><au>YAVITT, Joseph B</au><au>ZINDER, Stephen H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Methanoregula boonei gen. nov., sp. nov., an acidiphilic methanogen isolated from an acidic peat bog</atitle><jtitle>International journal of systematic and evolutionary microbiology</jtitle><addtitle>Int J Syst Evol Microbiol</addtitle><date>2011</date><risdate>2011</risdate><volume>61</volume><issue>Pt 1</issue><spage>45</spage><epage>52</epage><pages>45-52</pages><issn>1466-5026</issn><eissn>1466-5034</eissn><abstract>A novel acidiphilic, hydrogenotrophic methanogen, designated strain 6A8(T), was isolated from an acidic (pH 4.0-4.5) and ombrotrophic (rain-fed) bog located near Ithaca, NY, USA. Cultures were dimorphic, containing thin rods (0.2-0.3 μm in diameter and 0.8-3.0 μm long) and irregular cocci (0.2-0.8 μm in diameter). The culture utilized H(2)/CO(2) to produce methane but did not utilize formate, acetate, methanol, ethanol, 2-propanol, butanol or trimethylamine. Optimal growth conditions were near pH 5.1 and 35 °C. The culture grew in basal medium containing as little as 0.43 mM Na(+) and growth was inhibited completely by 50 mM NaCl. To our knowledge, strain 6A8(T) is one of the most acidiphilic (lowest pH optimum) and salt-sensitive methanogens in pure culture. Acetate, coenzyme M, vitamins and yeast extract were required for growth. It is proposed that a new genus and species be established for this organism, Methanoregula boonei gen. nov., sp. nov. The type strain of Methanoregula boonei is 6A8(T) (=DSM 21154(T) =JCM 14090(T)).</abstract><cop>Reading</cop><pub>Society for General Microbiology</pub><pmid>20154331</pmid><doi>10.1099/ijs.0.021782-0</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Bacteriology Biological and medical sciences Carbon Dioxide - metabolism Cluster Analysis Culture Media - chemistry DNA, Archaeal - chemistry DNA, Archaeal - genetics DNA, Ribosomal - chemistry DNA, Ribosomal - genetics Environmental Microbiology Fundamental and applied biological sciences. Psychology Hydrogen - metabolism Hydrogen-Ion Concentration Methane - metabolism Methanomicrobiales - classification Methanomicrobiales - genetics Methanomicrobiales - isolation & purification Methanomicrobiales - physiology Microbiology Molecular Sequence Data Phylogeny RNA, Ribosomal, 16S - genetics Sequence Analysis, DNA Sodium Chloride - metabolism Soil Systematics Temperature United States Wetlands |
| title | Methanoregula boonei gen. nov., sp. nov., an acidiphilic methanogen isolated from an acidic peat bog |
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