Characterization ofCandida albicans colony-morphology mutants and their hybrids
The collection wild-type strain ofCandida albicans was used to obtain auxotrophic and colony-morphology mutants by ‘nitrosoguanidine’ treatment. Intraspecific protoplast fusion induced by Ca2+-poly(ethyleneglycol) was carried out in various pairings between the auxotrophic strain producing smooth co...
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| Veröffentlicht in: | Folia microbiologica 2003-03, Vol.48 (2), p.203-209 |
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| creator | Novák, A. Vágvölgyi, C. Pesti, M. |
| description | The collection wild-type strain ofCandida albicans was used to obtain auxotrophic and colony-morphology mutants by ‘nitrosoguanidine’ treatment. Intraspecific protoplast fusion induced by Ca2+-poly(ethyleneglycol) was carried out in various pairings between the auxotrophic strain producing smooth colonies and containing blastospores and the colony-morphology mutants containing a mixture of blastospores and pseudohyphae or only hyphae. Hybrids exhibiting full or partial complementation were obtained when mutants producing smooth colonies and colony-morphology variants of different origins were fused. The mutation responsible for the colony-morphology character (if various types of colony morphomutants were crossed) proved to be recessive or semidominant. Representative hybrids exhibited elevated DNA contents as measured by flow cytometry. To illustrate various cell types, and especially the intermediate one (never observed in natural isolates), a preparation method was further developed for scanning electron microscopic studies. |
| doi_str_mv | 10.1007/BF02930957 |
| format | Article |
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Intraspecific protoplast fusion induced by Ca2+-poly(ethyleneglycol) was carried out in various pairings between the auxotrophic strain producing smooth colonies and containing blastospores and the colony-morphology mutants containing a mixture of blastospores and pseudohyphae or only hyphae. Hybrids exhibiting full or partial complementation were obtained when mutants producing smooth colonies and colony-morphology variants of different origins were fused. The mutation responsible for the colony-morphology character (if various types of colony morphomutants were crossed) proved to be recessive or semidominant. Representative hybrids exhibited elevated DNA contents as measured by flow cytometry. To illustrate various cell types, and especially the intermediate one (never observed in natural isolates), a preparation method was further developed for scanning electron microscopic studies.</description><identifier>ISSN: 0015-5632</identifier><identifier>EISSN: 1874-9356</identifier><identifier>DOI: 10.1007/BF02930957</identifier><language>eng</language><publisher>Dordrecht: Springer Nature B.V</publisher><subject>Bacteria ; Calcium ions ; Colonies ; Colonies & territories ; Flow cytometry ; Hybrids ; Hyphae ; Morphology ; Mutants ; Mutation ; Protoplast fusion ; Pseudohyphae ; Scanning electron microscopy</subject><ispartof>Folia microbiologica, 2003-03, Vol.48 (2), p.203-209</ispartof><rights>Institute of Microbiology, Academy of Sciences of the Czech Republic 2003.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c1358-40d2aa8cff4df86d25259fde8b7136a5d7b5cff7a0a2333586d797eb52a48c6f3</citedby><cites>FETCH-LOGICAL-c1358-40d2aa8cff4df86d25259fde8b7136a5d7b5cff7a0a2333586d797eb52a48c6f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids></links><search><creatorcontrib>Novák, A.</creatorcontrib><creatorcontrib>Vágvölgyi, C.</creatorcontrib><creatorcontrib>Pesti, M.</creatorcontrib><title>Characterization ofCandida albicans colony-morphology mutants and their hybrids</title><title>Folia microbiologica</title><description>The collection wild-type strain ofCandida albicans was used to obtain auxotrophic and colony-morphology mutants by ‘nitrosoguanidine’ treatment. 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| subjects | Bacteria Calcium ions Colonies Colonies & territories Flow cytometry Hybrids Hyphae Morphology Mutants Mutation Protoplast fusion Pseudohyphae Scanning electron microscopy |
| title | Characterization ofCandida albicans colony-morphology mutants and their hybrids |
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