Enrichment culture and molecular identification of diverse Bartonella species in stray dogs
Using pre-enrichment culture in Bartonella alpha- Proteobacteria growth medium (BAPGM) followed by PCR amplification and DNA sequence identification that targeted a fragment of the citrate synthase gene ( gltA), we provide evidence of common bartonella infections and diverse Bartonella species in th...
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| Veröffentlicht in: | Veterinary microbiology 2010-12, Vol.146 (3), p.314-319 |
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| creator | Bai, Ying Kosoy, Michael Y. Boonmar, Sumalee Sawatwong, Pongpun Sangmaneedet, Somboon Peruski, Leonard F. |
| description | Using pre-enrichment culture in
Bartonella alpha-
Proteobacteria growth medium (BAPGM) followed by PCR amplification and DNA sequence identification that targeted a fragment of the citrate synthase gene (
gltA), we provide evidence of common bartonella infections and diverse
Bartonella species in the blood of stray dogs from Bangkok and Khon Kaen, Thailand. The overall prevalence of all
Bartonella species was 31.3% (60/192), with 27.9% (31/111) and 35.8% (29/81) in the stray dogs from Bangkok and Khon Kaen, respectively. Phylogenetic analyzes of
gltA identified eight species/genotypes of
Bartonella in the blood of stray dogs, including
B. vinsonii subsp.
arupensis,
B. elizabethae,
B. grahamii,
B. quintana,
B. taylorii, and three novel genotypes (BK1, KK1 and KK2) possibly representing unique species with ≤90.2% similarities to any of the known
Bartonella species
B. vinsonii subsp.
arupensis was the only species detected in dogs from both sites,
B. quintana and BK1 were found in the dogs from Bangkok,
B. elizabethae,
B. taylorii, KK1 and KK2 were found in the dogs from Khon Kaen. We conclude that stray dogs in Thailand are frequently infected with
Bartonella species that vary with geographic region. As some
Bartonella species detected in the present study are considered pathogenic for humans, stray dogs in Thailand may serve as possible reservoirs for
Bartonella causing human illnesses. Further work is needed to determine the role of those newly discovered
Bartonella genotypes/species in human and veterinary medicine. |
| doi_str_mv | 10.1016/j.vetmic.2010.05.017 |
| format | Article |
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Bartonella alpha-
Proteobacteria growth medium (BAPGM) followed by PCR amplification and DNA sequence identification that targeted a fragment of the citrate synthase gene (
gltA), we provide evidence of common bartonella infections and diverse
Bartonella species in the blood of stray dogs from Bangkok and Khon Kaen, Thailand. The overall prevalence of all
Bartonella species was 31.3% (60/192), with 27.9% (31/111) and 35.8% (29/81) in the stray dogs from Bangkok and Khon Kaen, respectively. Phylogenetic analyzes of
gltA identified eight species/genotypes of
Bartonella in the blood of stray dogs, including
B. vinsonii subsp.
arupensis,
B. elizabethae,
B. grahamii,
B. quintana,
B. taylorii, and three novel genotypes (BK1, KK1 and KK2) possibly representing unique species with ≤90.2% similarities to any of the known
Bartonella species
B. vinsonii subsp.
arupensis was the only species detected in dogs from both sites,
B. quintana and BK1 were found in the dogs from Bangkok,
B. elizabethae,
B. taylorii, KK1 and KK2 were found in the dogs from Khon Kaen. We conclude that stray dogs in Thailand are frequently infected with
Bartonella species that vary with geographic region. As some
Bartonella species detected in the present study are considered pathogenic for humans, stray dogs in Thailand may serve as possible reservoirs for
Bartonella causing human illnesses. Further work is needed to determine the role of those newly discovered
Bartonella genotypes/species in human and veterinary medicine.</description><identifier>ISSN: 0378-1135</identifier><identifier>EISSN: 1873-2542</identifier><identifier>DOI: 10.1016/j.vetmic.2010.05.017</identifier><identifier>PMID: 20570065</identifier><identifier>CODEN: VMICDQ</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; bacterial infections ; Bacteriology ; BAPGM ; Bartonella ; Bartonella - classification ; Bartonella - genetics ; Bartonella - growth & development ; Bartonella Infections - epidemiology ; Bartonella Infections - microbiology ; Bartonella Infections - veterinary ; Biological and medical sciences ; Citrate (si)-Synthase - genetics ; culture media ; disease prevalence ; disease reservoirs ; DNA ; DNA, Bacterial - genetics ; Dog Diseases - epidemiology ; Dog Diseases - microbiology ; Dogs ; Fundamental and applied biological sciences. Psychology ; Genotype ; geographical variation ; gltA ; Humans ; microbial genetics ; microbial identification ; Microbiology ; Miscellaneous ; Molecular Sequence Data ; nucleotide sequences ; pathogen identification ; Phylogeny ; polymerase chain reaction ; Pre-enrichment ; Prevalence ; species diversity ; stray animals ; Stray dogs ; Thailand - epidemiology ; zoonoses</subject><ispartof>Veterinary microbiology, 2010-12, Vol.146 (3), p.314-319</ispartof><rights>2010</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2010 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c447t-365810b3066ef503759222ced86e14fcad9f77f17df2b3ba8a990113442831573</citedby><cites>FETCH-LOGICAL-c447t-365810b3066ef503759222ced86e14fcad9f77f17df2b3ba8a990113442831573</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0378113510002440$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23669237$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20570065$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bai, Ying</creatorcontrib><creatorcontrib>Kosoy, Michael Y.</creatorcontrib><creatorcontrib>Boonmar, Sumalee</creatorcontrib><creatorcontrib>Sawatwong, Pongpun</creatorcontrib><creatorcontrib>Sangmaneedet, Somboon</creatorcontrib><creatorcontrib>Peruski, Leonard F.</creatorcontrib><title>Enrichment culture and molecular identification of diverse Bartonella species in stray dogs</title><title>Veterinary microbiology</title><addtitle>Vet Microbiol</addtitle><description>Using pre-enrichment culture in
Bartonella alpha-
Proteobacteria growth medium (BAPGM) followed by PCR amplification and DNA sequence identification that targeted a fragment of the citrate synthase gene (
gltA), we provide evidence of common bartonella infections and diverse
Bartonella species in the blood of stray dogs from Bangkok and Khon Kaen, Thailand. The overall prevalence of all
Bartonella species was 31.3% (60/192), with 27.9% (31/111) and 35.8% (29/81) in the stray dogs from Bangkok and Khon Kaen, respectively. Phylogenetic analyzes of
gltA identified eight species/genotypes of
Bartonella in the blood of stray dogs, including
B. vinsonii subsp.
arupensis,
B. elizabethae,
B. grahamii,
B. quintana,
B. taylorii, and three novel genotypes (BK1, KK1 and KK2) possibly representing unique species with ≤90.2% similarities to any of the known
Bartonella species
B. vinsonii subsp.
arupensis was the only species detected in dogs from both sites,
B. quintana and BK1 were found in the dogs from Bangkok,
B. elizabethae,
B. taylorii, KK1 and KK2 were found in the dogs from Khon Kaen. We conclude that stray dogs in Thailand are frequently infected with
Bartonella species that vary with geographic region. As some
Bartonella species detected in the present study are considered pathogenic for humans, stray dogs in Thailand may serve as possible reservoirs for
Bartonella causing human illnesses. Further work is needed to determine the role of those newly discovered
Bartonella genotypes/species in human and veterinary medicine.</description><subject>Animals</subject><subject>bacterial infections</subject><subject>Bacteriology</subject><subject>BAPGM</subject><subject>Bartonella</subject><subject>Bartonella - classification</subject><subject>Bartonella - genetics</subject><subject>Bartonella - growth & development</subject><subject>Bartonella Infections - epidemiology</subject><subject>Bartonella Infections - microbiology</subject><subject>Bartonella Infections - veterinary</subject><subject>Biological and medical sciences</subject><subject>Citrate (si)-Synthase - genetics</subject><subject>culture media</subject><subject>disease prevalence</subject><subject>disease reservoirs</subject><subject>DNA</subject><subject>DNA, Bacterial - genetics</subject><subject>Dog Diseases - epidemiology</subject><subject>Dog Diseases - microbiology</subject><subject>Dogs</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genotype</subject><subject>geographical variation</subject><subject>gltA</subject><subject>Humans</subject><subject>microbial genetics</subject><subject>microbial identification</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Molecular Sequence Data</subject><subject>nucleotide sequences</subject><subject>pathogen identification</subject><subject>Phylogeny</subject><subject>polymerase chain reaction</subject><subject>Pre-enrichment</subject><subject>Prevalence</subject><subject>species diversity</subject><subject>stray animals</subject><subject>Stray dogs</subject><subject>Thailand - epidemiology</subject><subject>zoonoses</subject><issn>0378-1135</issn><issn>1873-2542</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUuPFCEURonROO3oPzDKxriqlkfx2pjoZHwkk7jQWbkgNHUZ6VQVLVCdzL-XTrW60xUBzr3344DQc0q2lFD5Zr89Qp2i3zLSjojYEqoeoA3VindM9Owh2hCudEcpFxfoSSl7QkhvJHmMLhgRihApNuj79Zyj_zHBXLFfxrpkwG4e8JRGaHuXcRzaXQzRuxrTjFPAQzxCLoDfu1zTDOPocDmAj1BwnHGp2d3jId2Vp-hRcGOBZ-f1Et1-uP529am7-fLx89W7m873vaodl0JTsuNESgiiZRaGMeZh0BJoH7wbTFAqUDUEtuM7p50xpL2q75nmVCh-iV6vfQ85_VygVDvF4k-5ZkhLsZobabSk_f9JyihXhvJG9ivpcyolQ7CHHCeX7y0l9uTf7u3q3578WyJs89_KXpwHLLsJhj9Fv4U34NUZcMW7MWQ3-1j-clxKw_ip0cuVCy5Zd5cbc_u1TeKEGsq01o14uxLQ1B4jZFvaF8zNW8zgqx1S_HfWX3_triI</recordid><startdate>20101215</startdate><enddate>20101215</enddate><creator>Bai, Ying</creator><creator>Kosoy, Michael Y.</creator><creator>Boonmar, Sumalee</creator><creator>Sawatwong, Pongpun</creator><creator>Sangmaneedet, Somboon</creator><creator>Peruski, Leonard F.</creator><general>Elsevier B.V</general><general>Amsterdam; New York: Elsevier</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>20101215</creationdate><title>Enrichment culture and molecular identification of diverse Bartonella species in stray dogs</title><author>Bai, Ying ; Kosoy, Michael Y. ; Boonmar, Sumalee ; Sawatwong, Pongpun ; Sangmaneedet, Somboon ; Peruski, Leonard F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c447t-365810b3066ef503759222ced86e14fcad9f77f17df2b3ba8a990113442831573</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>bacterial infections</topic><topic>Bacteriology</topic><topic>BAPGM</topic><topic>Bartonella</topic><topic>Bartonella - classification</topic><topic>Bartonella - genetics</topic><topic>Bartonella - growth & development</topic><topic>Bartonella Infections - epidemiology</topic><topic>Bartonella Infections - microbiology</topic><topic>Bartonella Infections - veterinary</topic><topic>Biological and medical sciences</topic><topic>Citrate (si)-Synthase - genetics</topic><topic>culture media</topic><topic>disease prevalence</topic><topic>disease reservoirs</topic><topic>DNA</topic><topic>DNA, Bacterial - genetics</topic><topic>Dog Diseases - epidemiology</topic><topic>Dog Diseases - microbiology</topic><topic>Dogs</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genotype</topic><topic>geographical variation</topic><topic>gltA</topic><topic>Humans</topic><topic>microbial genetics</topic><topic>microbial identification</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Molecular Sequence Data</topic><topic>nucleotide sequences</topic><topic>pathogen identification</topic><topic>Phylogeny</topic><topic>polymerase chain reaction</topic><topic>Pre-enrichment</topic><topic>Prevalence</topic><topic>species diversity</topic><topic>stray animals</topic><topic>Stray dogs</topic><topic>Thailand - epidemiology</topic><topic>zoonoses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bai, Ying</creatorcontrib><creatorcontrib>Kosoy, Michael Y.</creatorcontrib><creatorcontrib>Boonmar, Sumalee</creatorcontrib><creatorcontrib>Sawatwong, Pongpun</creatorcontrib><creatorcontrib>Sangmaneedet, Somboon</creatorcontrib><creatorcontrib>Peruski, Leonard F.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Veterinary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bai, Ying</au><au>Kosoy, Michael Y.</au><au>Boonmar, Sumalee</au><au>Sawatwong, Pongpun</au><au>Sangmaneedet, Somboon</au><au>Peruski, Leonard F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enrichment culture and molecular identification of diverse Bartonella species in stray dogs</atitle><jtitle>Veterinary microbiology</jtitle><addtitle>Vet Microbiol</addtitle><date>2010-12-15</date><risdate>2010</risdate><volume>146</volume><issue>3</issue><spage>314</spage><epage>319</epage><pages>314-319</pages><issn>0378-1135</issn><eissn>1873-2542</eissn><coden>VMICDQ</coden><abstract>Using pre-enrichment culture in
Bartonella alpha-
Proteobacteria growth medium (BAPGM) followed by PCR amplification and DNA sequence identification that targeted a fragment of the citrate synthase gene (
gltA), we provide evidence of common bartonella infections and diverse
Bartonella species in the blood of stray dogs from Bangkok and Khon Kaen, Thailand. The overall prevalence of all
Bartonella species was 31.3% (60/192), with 27.9% (31/111) and 35.8% (29/81) in the stray dogs from Bangkok and Khon Kaen, respectively. Phylogenetic analyzes of
gltA identified eight species/genotypes of
Bartonella in the blood of stray dogs, including
B. vinsonii subsp.
arupensis,
B. elizabethae,
B. grahamii,
B. quintana,
B. taylorii, and three novel genotypes (BK1, KK1 and KK2) possibly representing unique species with ≤90.2% similarities to any of the known
Bartonella species
B. vinsonii subsp.
arupensis was the only species detected in dogs from both sites,
B. quintana and BK1 were found in the dogs from Bangkok,
B. elizabethae,
B. taylorii, KK1 and KK2 were found in the dogs from Khon Kaen. We conclude that stray dogs in Thailand are frequently infected with
Bartonella species that vary with geographic region. As some
Bartonella species detected in the present study are considered pathogenic for humans, stray dogs in Thailand may serve as possible reservoirs for
Bartonella causing human illnesses. Further work is needed to determine the role of those newly discovered
Bartonella genotypes/species in human and veterinary medicine.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>20570065</pmid><doi>10.1016/j.vetmic.2010.05.017</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Veterinary microbiology, 2010-12, Vol.146 (3), p.314-319 |
| issn | 0378-1135 1873-2542 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals bacterial infections Bacteriology BAPGM Bartonella Bartonella - classification Bartonella - genetics Bartonella - growth & development Bartonella Infections - epidemiology Bartonella Infections - microbiology Bartonella Infections - veterinary Biological and medical sciences Citrate (si)-Synthase - genetics culture media disease prevalence disease reservoirs DNA DNA, Bacterial - genetics Dog Diseases - epidemiology Dog Diseases - microbiology Dogs Fundamental and applied biological sciences. Psychology Genotype geographical variation gltA Humans microbial genetics microbial identification Microbiology Miscellaneous Molecular Sequence Data nucleotide sequences pathogen identification Phylogeny polymerase chain reaction Pre-enrichment Prevalence species diversity stray animals Stray dogs Thailand - epidemiology zoonoses |
| title | Enrichment culture and molecular identification of diverse Bartonella species in stray dogs |
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