DNA Detection on a Power-free Microchip with Laminar Flow-assisted Dendritic Amplification
In this paper, we describe DNA detection experiments using our two original technologies, power-free microchip and laminar flow-assisted dendritic amplification (LFDA), which were previously applied to immunoassays. A microchip was fabricated by combining a poly(dimethylsiloxane) (PDMS) part having...
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| Veröffentlicht in: | Analytical Sciences 2010/10/10, Vol.26(10), pp.1053-1057 |
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| creator | HOSOKAWA, Kazuo SATO, Takahiro SATO, Yasunobu MAEDA, Mizuo |
| description | In this paper, we describe DNA detection experiments using our two original technologies, power-free microchip and laminar flow-assisted dendritic amplification (LFDA), which were previously applied to immunoassays. A microchip was fabricated by combining a poly(dimethylsiloxane) (PDMS) part having microchannel patterns and a glass plate modified with probe DNA. We carried out two kinds of experiments: the detection of 21-base biotinylated target DNA and the detection of single-nucleotide polymorphism (SNP) in 56-base unlabeled target DNA by sandwich hybridization with biotinylated probe DNA. For both of the experiments, the necessary solutions were injected into microchannels not by an external power source, but by air dissolution into the PDMS part. After a hybridization reaction, the LFDA was started by injecting FITC-labeled streptavidin and biotinylated anti-streptavidin antibody onto the reaction site. With a detection time of 20 min, the limit of detection (LOD) for the biotinylated target was 2.2 pM, and the LOD for the SNP was 10 – 30 pM, depending on the SNP type. |
| doi_str_mv | 10.2116/analsci.26.1053 |
| format | Article |
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A microchip was fabricated by combining a poly(dimethylsiloxane) (PDMS) part having microchannel patterns and a glass plate modified with probe DNA. We carried out two kinds of experiments: the detection of 21-base biotinylated target DNA and the detection of single-nucleotide polymorphism (SNP) in 56-base unlabeled target DNA by sandwich hybridization with biotinylated probe DNA. For both of the experiments, the necessary solutions were injected into microchannels not by an external power source, but by air dissolution into the PDMS part. After a hybridization reaction, the LFDA was started by injecting FITC-labeled streptavidin and biotinylated anti-streptavidin antibody onto the reaction site. 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With a detection time of 20 min, the limit of detection (LOD) for the biotinylated target was 2.2 pM, and the LOD for the SNP was 10 – 30 pM, depending on the SNP type.</description><subject>Analytical Chemistry</subject><subject>Biotinylation</subject><subject>Chemistry</subject><subject>Dimethylpolysiloxanes</subject><subject>DNA - analysis</subject><subject>DNA - genetics</subject><subject>DNA Probes</subject><subject>Fluorescein-5-isothiocyanate</subject><subject>Humans</subject><subject>Immunoassay</subject><subject>Lab-On-A-Chip Devices</subject><subject>Nucleic Acid Hybridization</subject><subject>Polymorphism, Single Nucleotide</subject><subject>Streptavidin</subject><issn>0910-6340</issn><issn>1348-2246</issn><issn>1348-2246</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtvEzEURi1ERdPCmh0aiQWrSf0eexm1DUUKhQVs2Fi3njuNo3kEe6KIf49HCRGthJAse-HzHVv3I-Qto3POmL6CHtrkw5zrOaNKvCAzJqQpOZf6JZlRy2iphaTn5CKlDaWMG85fkXNOrRJUVjPy4-Z-UdzgiH4MQ1_kBcXXYY-xbCJi8Tn4OPh12Bb7MK6LFXShh1gs22FfQkohjVjneF_HMAZfLLptG5rgYZK9JmdN_h2-OZ6X5Pvy9tv1Xbn68vHT9WJVesX1WNaKgZcAulKGUWwqsIBMa2moFVY3zNaUVrWprRagH0yNqKgyRoragjcgLsmHg3cbh587TKPrQvLYttDjsEvOZIsVQqn_kpWyTCgqaCbfPyM3wy5Ow3ZMSqOqilVVpq4OVB5SShEbt42hg_jLMeqmftyxH8e1m_rJiXdH7-6hw_rE_ykkA_QApHzVP2L86-F_OpeHyCaN8IgnJ8RcSYtP-eM-BU-AX0N02IvfuGu07A</recordid><startdate>2010</startdate><enddate>2010</enddate><creator>HOSOKAWA, Kazuo</creator><creator>SATO, Takahiro</creator><creator>SATO, Yasunobu</creator><creator>MAEDA, Mizuo</creator><general>The Japan Society for Analytical Chemistry</general><general>Springer Nature Singapore</general><general>Japan Science and Technology Agency</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SE</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>FR3</scope><scope>H8G</scope><scope>JG9</scope><scope>L7M</scope><scope>P64</scope><scope>7X8</scope><scope>7TM</scope></search><sort><creationdate>2010</creationdate><title>DNA Detection on a Power-free Microchip with Laminar Flow-assisted Dendritic Amplification</title><author>HOSOKAWA, Kazuo ; SATO, Takahiro ; SATO, Yasunobu ; MAEDA, Mizuo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c526t-d51ac4aa675810ef7a9ae1664809396f19d007d8d963a6b8dee5058843d9ac8a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Analytical Chemistry</topic><topic>Biotinylation</topic><topic>Chemistry</topic><topic>Dimethylpolysiloxanes</topic><topic>DNA - analysis</topic><topic>DNA - genetics</topic><topic>DNA Probes</topic><topic>Fluorescein-5-isothiocyanate</topic><topic>Humans</topic><topic>Immunoassay</topic><topic>Lab-On-A-Chip Devices</topic><topic>Nucleic Acid Hybridization</topic><topic>Polymorphism, Single Nucleotide</topic><topic>Streptavidin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>HOSOKAWA, Kazuo</creatorcontrib><creatorcontrib>SATO, Takahiro</creatorcontrib><creatorcontrib>SATO, Yasunobu</creatorcontrib><creatorcontrib>MAEDA, Mizuo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Nucleic Acids Abstracts</collection><jtitle>Analytical Sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>HOSOKAWA, Kazuo</au><au>SATO, Takahiro</au><au>SATO, Yasunobu</au><au>MAEDA, Mizuo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DNA Detection on a Power-free Microchip with Laminar Flow-assisted Dendritic Amplification</atitle><jtitle>Analytical Sciences</jtitle><stitle>ANAL. 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| subjects | Analytical Chemistry Biotinylation Chemistry Dimethylpolysiloxanes DNA - analysis DNA - genetics DNA Probes Fluorescein-5-isothiocyanate Humans Immunoassay Lab-On-A-Chip Devices Nucleic Acid Hybridization Polymorphism, Single Nucleotide Streptavidin |
| title | DNA Detection on a Power-free Microchip with Laminar Flow-assisted Dendritic Amplification |
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