Robust one-day in situ hybridization protocol for detection of microRNAs in paraffin samples using LNA probes

MicroRNAs (miRNAs) constitute a group of small non-coding RNA molecules generally 18–22 base-pairs in length. miRNAs are considered to be negative regulators of gene expression at the level of post-transcription and are important in normal physiological development, tissue homeostasis and disease. T...

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Veröffentlicht in:	Methods (San Diego, Calif.) Calif.), 2010-12, Vol.52 (4), p.375-381
Hauptverfasser:	Jørgensen, Stine, Baker, Adam, Møller, Søren, Nielsen, Boye Schnack
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Breast Neoplasms - chemistry Endopeptidase K - metabolism FFPE Humans In situ hybridization In Situ Hybridization - methods LNA Mice microRNA MicroRNAs - analysis Oligonucleotides Paraffin Embedding RNA, Small Nuclear Sensitivity and Specificity
Online-Zugang:	Volltext
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