Screening of a specific monoclonal antibody against and detection ofListeria monocytogenes whole cells using a surface plasmon resonance biosensor
In this study, a specific monoclonal antibody againstListeria monocytogenes was screened using an SPR biosensor Monoclonal antibodies were bound to protein L, after which theL. monocytogenes cells were subjected to an affinity assay. Protein L was immobilized on a carboxymethyl dextran (CM-Dex) surf...
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| Veröffentlicht in: | Biotechnology and bioprocess engineering 2007-04, Vol.12 (2), p.80-85 |
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| creator | Joung, Hyou-Arm Shim, Won-Bo Chung, Duck-Hwa Ahn, Junhyoung Chung, Bong Hyun Choi, Ho-Suk Ha, Sang-Do Kim, Keun-Sung Lee, Kyu-Ho Kim, Cheol-Ho Kim, Kwang-Yup Kim, Min-Gon |
| description | In this study, a specific monoclonal antibody againstListeria monocytogenes was screened using an SPR biosensor Monoclonal antibodies were bound to protein L, after which theL. monocytogenes cells were subjected to an affinity assay. Protein L was immobilized on a carboxymethyl dextran (CM-Dex) surface via an amine coupling method and utilized repeatedly by regeneration. The monoclonal antibody, 'A18', was selected and employed for the high-sensitivity detection ofL. monocytogenes. Under optimized conditions, 10^sup 3^ cells/ml or 50 cells were detected by the SPR biosensor.[PUBLICATION ABSTRACT] |
| doi_str_mv | 10.1007/BF03028630 |
| format | Article |
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Protein L was immobilized on a carboxymethyl dextran (CM-Dex) surface via an amine coupling method and utilized repeatedly by regeneration. The monoclonal antibody, 'A18', was selected and employed for the high-sensitivity detection ofL. monocytogenes. Under optimized conditions, 10^sup 3^ cells/ml or 50 cells were detected by the SPR biosensor.[PUBLICATION ABSTRACT]</description><identifier>ISSN: 1226-8372</identifier><identifier>EISSN: 1976-3816</identifier><identifier>DOI: 10.1007/BF03028630</identifier><language>eng</language><publisher>Dordrecht: Springer Nature B.V</publisher><subject>amines ; Biosensors ; Coupling methods ; Dextran ; Monoclonal antibodies ; protein L ; surface plasmon resonance</subject><ispartof>Biotechnology and bioprocess engineering, 2007-04, Vol.12 (2), p.80-85</ispartof><rights>The Korean Society for Biotechnology and Bioengineering 2007</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c1353-4d86700bbeeea844e1ba92ab721413c94d52f93e357ce474daa4b972186b235e3</citedby><cites>FETCH-LOGICAL-c1353-4d86700bbeeea844e1ba92ab721413c94d52f93e357ce474daa4b972186b235e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids></links><search><creatorcontrib>Joung, Hyou-Arm</creatorcontrib><creatorcontrib>Shim, Won-Bo</creatorcontrib><creatorcontrib>Chung, Duck-Hwa</creatorcontrib><creatorcontrib>Ahn, Junhyoung</creatorcontrib><creatorcontrib>Chung, Bong Hyun</creatorcontrib><creatorcontrib>Choi, Ho-Suk</creatorcontrib><creatorcontrib>Ha, Sang-Do</creatorcontrib><creatorcontrib>Kim, Keun-Sung</creatorcontrib><creatorcontrib>Lee, Kyu-Ho</creatorcontrib><creatorcontrib>Kim, Cheol-Ho</creatorcontrib><creatorcontrib>Kim, Kwang-Yup</creatorcontrib><creatorcontrib>Kim, Min-Gon</creatorcontrib><title>Screening of a specific monoclonal antibody against and detection ofListeria monocytogenes whole cells using a surface plasmon resonance biosensor</title><title>Biotechnology and bioprocess engineering</title><description>In this study, a specific monoclonal antibody againstListeria monocytogenes was screened using an SPR biosensor Monoclonal antibodies were bound to protein L, after which theL. monocytogenes cells were subjected to an affinity assay. 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| fulltext | fulltext |
| identifier | ISSN: 1226-8372 |
| ispartof | Biotechnology and bioprocess engineering, 2007-04, Vol.12 (2), p.80-85 |
| issn | 1226-8372 1976-3816 |
| language | eng |
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| source | Springer Nature - Complete Springer Journals; EZB-FREE-00999 freely available EZB journals |
| subjects | amines Biosensors Coupling methods Dextran Monoclonal antibodies protein L surface plasmon resonance |
| title | Screening of a specific monoclonal antibody against and detection ofListeria monocytogenes whole cells using a surface plasmon resonance biosensor |
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