MORPHEUS' MOLECULE1 Is Required to Prevent Aberrant RNA Transcriptional Read-Through in Arabidopsis
Several pathways function to remove aberrant mRNA in eukaryotic cells; however, the exact mechanisms underlying the restriction of aberrant mRNA transcription are poorly understood. In this study, we found that MORPHEUS' MOLECULE1 (MOM1) is a key component of this regulatory machinery. The Arab...
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| Veröffentlicht in: | Plant physiology (Bethesda) 2010-11, Vol.154 (3), p.1272-1280 |
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| creator | Zhou, Yue Zhang, Jun Lin, Huixin Guo, Guangqin Guo, Yan |
| description | Several pathways function to remove aberrant mRNA in eukaryotic cells; however, the exact mechanisms underlying the restriction of aberrant mRNA transcription are poorly understood. In this study, we found that MORPHEUS' MOLECULE1 (MOM1) is a key component of this regulatory machinery. The Arabidopsis (Arabidopsis thaliana) mom1-44 mutation was identified by luciferase imaging in transgenic plants harboring a cauliflower mosaic virus 35S promoter-LUCIFERASE transgene lacking the 3'-untranslated region. In the mom1-44 mutant, transcriptional read-though occurred in genes with an aberrant RNA structure. Analysis of an RNA-dependent RNA polymerase2 mom1 double mutant revealed that the RNA-directed DNA methylation pathway is not involved in this regulatory process. Moreover, the prevention of aberrant mRNA transcriptional read-through by MOM1 is gene locus and transgene copy number independent. |
| doi_str_mv | 10.1104/pp.110.162131 |
| format | Article |
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In this study, we found that MORPHEUS' MOLECULE1 (MOM1) is a key component of this regulatory machinery. The Arabidopsis (Arabidopsis thaliana) mom1-44 mutation was identified by luciferase imaging in transgenic plants harboring a cauliflower mosaic virus 35S promoter-LUCIFERASE transgene lacking the 3'-untranslated region. In the mom1-44 mutant, transcriptional read-though occurred in genes with an aberrant RNA structure. Analysis of an RNA-dependent RNA polymerase2 mom1 double mutant revealed that the RNA-directed DNA methylation pathway is not involved in this regulatory process. Moreover, the prevention of aberrant mRNA transcriptional read-through by MOM1 is gene locus and transgene copy number independent.</description><identifier>ISSN: 0032-0889</identifier><identifier>ISSN: 1532-2548</identifier><identifier>EISSN: 1532-2548</identifier><identifier>DOI: 10.1104/pp.110.162131</identifier><identifier>PMID: 20826701</identifier><identifier>CODEN: PPHYA5</identifier><language>eng</language><publisher>Rockville, MD: American Society of Plant Biologists</publisher><subject>3' Untranslated Regions ; Arabidopsis - genetics ; Arabidopsis - metabolism ; Arabidopsis Proteins - genetics ; Arabidopsis Proteins - metabolism ; Arabidopsis thaliana ; Biological and medical sciences ; Cauliflower mosaic virus ; Cloning, Molecular ; Complementary DNA ; DNA Methylation ; ENVIRONMENTAL STRESS AND ADAPTATION TO STRESS ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Plant ; Genes ; Genetic mutation ; Imaging ; Luminescence ; Messenger RNA ; Mutation ; Nuclear Proteins - genetics ; Nuclear Proteins - metabolism ; Plant cells ; Plant physiology and development ; Plants, Genetically Modified - genetics ; Plants, Genetically Modified - metabolism ; Polyadenylation ; RNA ; RNA, Plant - biosynthesis ; Seedlings ; Small interfering RNA ; Transcription Factors - genetics ; Transcription Factors - metabolism ; Transcription, Genetic</subject><ispartof>Plant physiology (Bethesda), 2010-11, Vol.154 (3), p.1272-1280</ispartof><rights>2010 American Society of Plant Biologists</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c505t-35b40c3262f37b4c88b6ee267c05f450884cbac6be1a82a2b57f7a97e9adf4b83</citedby><cites>FETCH-LOGICAL-c505t-35b40c3262f37b4c88b6ee267c05f450884cbac6be1a82a2b57f7a97e9adf4b83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/25758674$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/25758674$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23423147$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20826701$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhou, Yue</creatorcontrib><creatorcontrib>Zhang, Jun</creatorcontrib><creatorcontrib>Lin, Huixin</creatorcontrib><creatorcontrib>Guo, Guangqin</creatorcontrib><creatorcontrib>Guo, Yan</creatorcontrib><title>MORPHEUS' MOLECULE1 Is Required to Prevent Aberrant RNA Transcriptional Read-Through in Arabidopsis</title><title>Plant physiology (Bethesda)</title><addtitle>Plant Physiol</addtitle><description>Several pathways function to remove aberrant mRNA in eukaryotic cells; however, the exact mechanisms underlying the restriction of aberrant mRNA transcription are poorly understood. In this study, we found that MORPHEUS' MOLECULE1 (MOM1) is a key component of this regulatory machinery. The Arabidopsis (Arabidopsis thaliana) mom1-44 mutation was identified by luciferase imaging in transgenic plants harboring a cauliflower mosaic virus 35S promoter-LUCIFERASE transgene lacking the 3'-untranslated region. In the mom1-44 mutant, transcriptional read-though occurred in genes with an aberrant RNA structure. Analysis of an RNA-dependent RNA polymerase2 mom1 double mutant revealed that the RNA-directed DNA methylation pathway is not involved in this regulatory process. Moreover, the prevention of aberrant mRNA transcriptional read-through by MOM1 is gene locus and transgene copy number independent.</description><subject>3' Untranslated Regions</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - metabolism</subject><subject>Arabidopsis Proteins - genetics</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>Arabidopsis thaliana</subject><subject>Biological and medical sciences</subject><subject>Cauliflower mosaic virus</subject><subject>Cloning, Molecular</subject><subject>Complementary DNA</subject><subject>DNA Methylation</subject><subject>ENVIRONMENTAL STRESS AND ADAPTATION TO STRESS</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Plant</subject><subject>Genes</subject><subject>Genetic mutation</subject><subject>Imaging</subject><subject>Luminescence</subject><subject>Messenger RNA</subject><subject>Mutation</subject><subject>Nuclear Proteins - genetics</subject><subject>Nuclear Proteins - metabolism</subject><subject>Plant cells</subject><subject>Plant physiology and development</subject><subject>Plants, Genetically Modified - genetics</subject><subject>Plants, Genetically Modified - metabolism</subject><subject>Polyadenylation</subject><subject>RNA</subject><subject>RNA, Plant - biosynthesis</subject><subject>Seedlings</subject><subject>Small interfering RNA</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><subject>Transcription, Genetic</subject><issn>0032-0889</issn><issn>1532-2548</issn><issn>1532-2548</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1vEzEQxS0EoqFw5Aj4gnraMv5ae49RFGillFRpcl7ZXm_rahNv7V0k_nscbWiPPc2T5ufnmXkIfSZwSQjwH31_rJekpISRN2hGBKMFFVy9RTOArEGp6gx9SOkRADLD36MzCoqWEsgM2Zv15vZqubu7wDfr1XKxWy0Jvk54455GH12Dh4Bvo_vjDgOeGxejzmLze463WSUbfT_4cNBdfqCbYvsQw3j_gP0Bz6M2vgl98ukjetfqLrlPp3qOdj-X28VVsVr_ul7MV4UVIIaCCcPBMlrSlknDrVKmdC7PaUG0XOQ9uDXalsYRraimRshW6kq6SjctN4qdo4vJt4_haXRpqPc-Wdd1-uDCmGrFqlKRirJXSVlSriBfMZPFRNoYUoqurfvo9zr-rQnUxwDqvj_Wegog819PzqPZu-aZ_n_xDHw_ATpZ3bX5jNanF45xmjOSmfsycY9pCPGlL6RQpTwO9m3qtzrU-j5mj90dzR8AqUDSqmL_AD0XnjI</recordid><startdate>20101101</startdate><enddate>20101101</enddate><creator>Zhou, Yue</creator><creator>Zhang, Jun</creator><creator>Lin, Huixin</creator><creator>Guo, Guangqin</creator><creator>Guo, Yan</creator><general>American Society of Plant Biologists</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TM</scope></search><sort><creationdate>20101101</creationdate><title>MORPHEUS' MOLECULE1 Is Required to Prevent Aberrant RNA Transcriptional Read-Through in Arabidopsis</title><author>Zhou, Yue ; Zhang, Jun ; Lin, Huixin ; Guo, Guangqin ; Guo, Yan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c505t-35b40c3262f37b4c88b6ee267c05f450884cbac6be1a82a2b57f7a97e9adf4b83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>3' Untranslated Regions</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - metabolism</topic><topic>Arabidopsis Proteins - genetics</topic><topic>Arabidopsis Proteins - metabolism</topic><topic>Arabidopsis thaliana</topic><topic>Biological and medical sciences</topic><topic>Cauliflower mosaic virus</topic><topic>Cloning, Molecular</topic><topic>Complementary DNA</topic><topic>DNA Methylation</topic><topic>ENVIRONMENTAL STRESS AND ADAPTATION TO STRESS</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Plant</topic><topic>Genes</topic><topic>Genetic mutation</topic><topic>Imaging</topic><topic>Luminescence</topic><topic>Messenger RNA</topic><topic>Mutation</topic><topic>Nuclear Proteins - genetics</topic><topic>Nuclear Proteins - metabolism</topic><topic>Plant cells</topic><topic>Plant physiology and development</topic><topic>Plants, Genetically Modified - genetics</topic><topic>Plants, Genetically Modified - metabolism</topic><topic>Polyadenylation</topic><topic>RNA</topic><topic>RNA, Plant - biosynthesis</topic><topic>Seedlings</topic><topic>Small interfering RNA</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhou, Yue</creatorcontrib><creatorcontrib>Zhang, Jun</creatorcontrib><creatorcontrib>Lin, Huixin</creatorcontrib><creatorcontrib>Guo, Guangqin</creatorcontrib><creatorcontrib>Guo, Yan</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Nucleic Acids Abstracts</collection><jtitle>Plant physiology (Bethesda)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhou, Yue</au><au>Zhang, Jun</au><au>Lin, Huixin</au><au>Guo, Guangqin</au><au>Guo, Yan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>MORPHEUS' MOLECULE1 Is Required to Prevent Aberrant RNA Transcriptional Read-Through in Arabidopsis</atitle><jtitle>Plant physiology (Bethesda)</jtitle><addtitle>Plant Physiol</addtitle><date>2010-11-01</date><risdate>2010</risdate><volume>154</volume><issue>3</issue><spage>1272</spage><epage>1280</epage><pages>1272-1280</pages><issn>0032-0889</issn><issn>1532-2548</issn><eissn>1532-2548</eissn><coden>PPHYA5</coden><abstract>Several pathways function to remove aberrant mRNA in eukaryotic cells; however, the exact mechanisms underlying the restriction of aberrant mRNA transcription are poorly understood. In this study, we found that MORPHEUS' MOLECULE1 (MOM1) is a key component of this regulatory machinery. The Arabidopsis (Arabidopsis thaliana) mom1-44 mutation was identified by luciferase imaging in transgenic plants harboring a cauliflower mosaic virus 35S promoter-LUCIFERASE transgene lacking the 3'-untranslated region. In the mom1-44 mutant, transcriptional read-though occurred in genes with an aberrant RNA structure. Analysis of an RNA-dependent RNA polymerase2 mom1 double mutant revealed that the RNA-directed DNA methylation pathway is not involved in this regulatory process. Moreover, the prevention of aberrant mRNA transcriptional read-through by MOM1 is gene locus and transgene copy number independent.</abstract><cop>Rockville, MD</cop><pub>American Society of Plant Biologists</pub><pmid>20826701</pmid><doi>10.1104/pp.110.162131</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 3' Untranslated Regions Arabidopsis - genetics Arabidopsis - metabolism Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism Arabidopsis thaliana Biological and medical sciences Cauliflower mosaic virus Cloning, Molecular Complementary DNA DNA Methylation ENVIRONMENTAL STRESS AND ADAPTATION TO STRESS Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Plant Genes Genetic mutation Imaging Luminescence Messenger RNA Mutation Nuclear Proteins - genetics Nuclear Proteins - metabolism Plant cells Plant physiology and development Plants, Genetically Modified - genetics Plants, Genetically Modified - metabolism Polyadenylation RNA RNA, Plant - biosynthesis Seedlings Small interfering RNA Transcription Factors - genetics Transcription Factors - metabolism Transcription, Genetic |
| title | MORPHEUS' MOLECULE1 Is Required to Prevent Aberrant RNA Transcriptional Read-Through in Arabidopsis |
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