An in vitro transcription assay for probing drug-DNA interactions at individual drug sites

An in vitro transcription assay of drug-DNA interactions has been described and is based largely on the stable lac UV5-initiated transcription complex. This system utilizes a synchronized population of radiolabeled nascent RNA 10 nucleotides long. Reaction of this initiated transcription complex wit...

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Veröffentlicht in:	Molecular biotechnology 1998-08, Vol.10 (1), p.63-75
Hauptverfasser:	PHILLIPS, D. R, CULLINANE, C. M, CROTHERS, D. M
Format:	Artikel
Sprache:	eng
Schlagworte:	Assaying Autoradiography Base Sequence Binding sites Bioassays Biological and medical sciences Biotechnology Deoxyribonucleic acid Diverse techniques DNA DNA - genetics DNA - isolation & purification DNA - metabolism DNA Footprinting - methods DNA-directed RNA polymerase DNA-Directed RNA Polymerases - chemistry DNA-Directed RNA Polymerases - genetics DNA-Directed RNA Polymerases - metabolism E coli Elongation Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics Genetic Techniques Kinetics Methods. Procedures. Technologies Miscellaneous Molecular and cellular biology Molecular Sequence Data Nucleotide sequence Nucleotides Pharmaceutical Preparations - chemistry Pharmaceutical Preparations - metabolism Promoter Regions, Genetic Ribonucleic acid RNA RNA - chemistry RNA - genetics RNA - metabolism RNA polymerase Studies Synthetic digonucleotides and genes. Sequencing Transcription termination Transcription, Genetic
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