Detection of single-copy genes in DNA from transgenic plants by nonradioactive Southern blot analysis

Improvements to the sensitivity, speed, and reproducibility of digoxigenin (DIG)-labeled probes and chemiluminescent substrates makes these compounds increasingly popular to detect nucleic acids. High sensitivity and low background are essential in Southern blot analysis, particularly with plant DNA...

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Veröffentlicht in:	Molecular biotechnology 1997-02, Vol.7 (1), p.79-84
Hauptverfasser:	MCCABE, M. S, POWER, J. B, DE LAAT, A. M. M, DAVEY, M. R
Format:	Artikel
Sprache:	eng
Schlagworte:	analytical methods Biological and medical sciences Biotechnology Blotting, Southern Chemiluminescence Deoxyribonucleic acid Digoxigenin DNA DNA probes DNA, Plant - analysis esteroides Fundamental and applied biological sciences. Psychology Gene Dosage Genes Genes, Plant Genetic engineering Genetic technics Isotope Labeling Methods. Procedures. Technologies Nucleic acids Plants, Genetically Modified - genetics Polymerase chain reaction Probes Signal to noise ratio steroide steroids Studies Substrates technique analytique tecnicas analiticas Transgenic animals and transgenic plants Transgenic plants
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