Effect of covalent attachment of polyethylene glycol on immunogenicity and circulating life of bovine liver catalase
Methoxypolyethylene glycols of 1900 daltons (PEG-1900) or 5000 daltons (PEG-5000) were covalently attached to bovine liver catalase using 2,4,6-trichloro-s-triazine as the coupling agent. Rabbits were immunized by the intravenous and intramuscular routes with catalase modified by covalent attachment...
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| Veröffentlicht in: | The Journal of biological chemistry 1977-06, Vol.252 (11), p.3582-3586 |
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| container_issue | 11 |
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| container_title | The Journal of biological chemistry |
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| creator | Abuchowski, A McCoy, J R Palczuk, N C van Es, T Davis, F F |
| description | Methoxypolyethylene glycols of 1900 daltons (PEG-1900) or 5000 daltons (PEG-5000) were covalently attached to bovine liver
catalase using 2,4,6-trichloro-s-triazine as the coupling agent. Rabbits were immunized by the intravenous and intramuscular
routes with catalase modified by covalent attachment of PEG-1900 to 43% of the amino groups (PEG-1900-catalase). The intravenous
antiserum did not yield detectable antibodies against PEG-1900-catalase or native catalase, as determined by Ouchterlony and
complement fixation methods, whereas the intramuscular antiserum contained antibodies to both PEG-1900-catalase and catalase.
PEG-1900 did not react with either antiserum. Catalase was prepared in which PEG-5000 was attached to 40% of the amino groups
(PEG-5000-catalase). This catalase preparation did not react with either antiserum. PEG-1900-catalase retained 93% of its
enzymatic activity; PEG-5000-catalase retained 95%. PEG-5000-catalase resisted digestion by trypsin, chymotrypsin, and a protease
from Streptomyces griseus. PEG-1900-catalase and PEG-5000-catalase exhibited enhanced circulating lives in the blood of acatalasemic
mice during repetitive intravenous injections. No evidence was seen of an immune response to injections of the modified enzymes.
Mice injected repetitively with PEG-5000-catalase remained immune competent for unmodieied catalase, and no evidence of tissue
or organ damage was seen. |
| doi_str_mv | 10.1016/s0021-9258(17)40292-4 |
| format | Article |
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catalase using 2,4,6-trichloro-s-triazine as the coupling agent. Rabbits were immunized by the intravenous and intramuscular
routes with catalase modified by covalent attachment of PEG-1900 to 43% of the amino groups (PEG-1900-catalase). The intravenous
antiserum did not yield detectable antibodies against PEG-1900-catalase or native catalase, as determined by Ouchterlony and
complement fixation methods, whereas the intramuscular antiserum contained antibodies to both PEG-1900-catalase and catalase.
PEG-1900 did not react with either antiserum. Catalase was prepared in which PEG-5000 was attached to 40% of the amino groups
(PEG-5000-catalase). This catalase preparation did not react with either antiserum. PEG-1900-catalase retained 93% of its
enzymatic activity; PEG-5000-catalase retained 95%. PEG-5000-catalase resisted digestion by trypsin, chymotrypsin, and a protease
from Streptomyces griseus. PEG-1900-catalase and PEG-5000-catalase exhibited enhanced circulating lives in the blood of acatalasemic
mice during repetitive intravenous injections. No evidence was seen of an immune response to injections of the modified enzymes.
Mice injected repetitively with PEG-5000-catalase remained immune competent for unmodieied catalase, and no evidence of tissue
or organ damage was seen.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1016/s0021-9258(17)40292-4</identifier><identifier>PMID: 16907</identifier><language>eng</language><publisher>United States: American Society for Biochemistry and Molecular Biology</publisher><subject>Acatalasia ; Animals ; Antibody Formation - drug effects ; Catalase - blood ; Catalase - immunology ; Cattle ; Complement Fixation Tests ; Hot Temperature ; Hydrogen-Ion Concentration ; Immunodiffusion ; Liver ; Mice ; Molecular Weight ; Peptide Hydrolases ; Polyethylene Glycols - pharmacology ; Rabbits</subject><ispartof>The Journal of biological chemistry, 1977-06, Vol.252 (11), p.3582-3586</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c495t-1ed31129721bfbf77ac64467e6f6ddfddedadea44a59c982b3b428cce7e38c133</citedby><cites>FETCH-LOGICAL-c495t-1ed31129721bfbf77ac64467e6f6ddfddedadea44a59c982b3b428cce7e38c133</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16907$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Abuchowski, A</creatorcontrib><creatorcontrib>McCoy, J R</creatorcontrib><creatorcontrib>Palczuk, N C</creatorcontrib><creatorcontrib>van Es, T</creatorcontrib><creatorcontrib>Davis, F F</creatorcontrib><title>Effect of covalent attachment of polyethylene glycol on immunogenicity and circulating life of bovine liver catalase</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>Methoxypolyethylene glycols of 1900 daltons (PEG-1900) or 5000 daltons (PEG-5000) were covalently attached to bovine liver
catalase using 2,4,6-trichloro-s-triazine as the coupling agent. Rabbits were immunized by the intravenous and intramuscular
routes with catalase modified by covalent attachment of PEG-1900 to 43% of the amino groups (PEG-1900-catalase). The intravenous
antiserum did not yield detectable antibodies against PEG-1900-catalase or native catalase, as determined by Ouchterlony and
complement fixation methods, whereas the intramuscular antiserum contained antibodies to both PEG-1900-catalase and catalase.
PEG-1900 did not react with either antiserum. Catalase was prepared in which PEG-5000 was attached to 40% of the amino groups
(PEG-5000-catalase). This catalase preparation did not react with either antiserum. PEG-1900-catalase retained 93% of its
enzymatic activity; PEG-5000-catalase retained 95%. PEG-5000-catalase resisted digestion by trypsin, chymotrypsin, and a protease
from Streptomyces griseus. PEG-1900-catalase and PEG-5000-catalase exhibited enhanced circulating lives in the blood of acatalasemic
mice during repetitive intravenous injections. No evidence was seen of an immune response to injections of the modified enzymes.
Mice injected repetitively with PEG-5000-catalase remained immune competent for unmodieied catalase, and no evidence of tissue
or organ damage was seen.</description><subject>Acatalasia</subject><subject>Animals</subject><subject>Antibody Formation - drug effects</subject><subject>Catalase - blood</subject><subject>Catalase - immunology</subject><subject>Cattle</subject><subject>Complement Fixation Tests</subject><subject>Hot Temperature</subject><subject>Hydrogen-Ion Concentration</subject><subject>Immunodiffusion</subject><subject>Liver</subject><subject>Mice</subject><subject>Molecular Weight</subject><subject>Peptide Hydrolases</subject><subject>Polyethylene Glycols - pharmacology</subject><subject>Rabbits</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1977</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkF2L1TAQhoO46HH1F4gQEEQvqpkk_cilLOsHLHihgnchTSankbQ5NumR_ntbz6JzM8O87zsDDyEvgL0FBs27zBiHSvG6ew3tG8m44pV8QA7AOlGJGn48JId_lsfkSc4_2VZSwSNyBY1i7YGUW-_RFpo8telsIk6FmlKMHcZ93NanFFcsw7pJSI9xtSnSNNEwjsuUjjgFG8pKzeSoDbNdoilhOtIYPO7pPp3DlovhjDO1pphoMj4lV97EjM_u-zX5_uH2282n6u7Lx8837-8qK1VdKkAnALhqOfS-921rbCNl02LjG-e8c-iMQyOlqZVVHe9FL3lnLbYoOgtCXJNXl7unOf1aMBc9hmwxRjNhWrLuhGpkzdvNWF-Mdk45z-j1aQ6jmVcNTO-s9dcdpN5Bamj1X9Zabrnn9w-WfkT3P7XD3dSXF3UIx-F3mFH3IdkBR81rrgG0qDsu_gB3ZokK</recordid><startdate>19770610</startdate><enddate>19770610</enddate><creator>Abuchowski, A</creator><creator>McCoy, J R</creator><creator>Palczuk, N C</creator><creator>van Es, T</creator><creator>Davis, F F</creator><general>American Society for Biochemistry and Molecular Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19770610</creationdate><title>Effect of covalent attachment of polyethylene glycol on immunogenicity and circulating life of bovine liver catalase</title><author>Abuchowski, A ; McCoy, J R ; Palczuk, N C ; van Es, T ; Davis, F F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c495t-1ed31129721bfbf77ac64467e6f6ddfddedadea44a59c982b3b428cce7e38c133</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1977</creationdate><topic>Acatalasia</topic><topic>Animals</topic><topic>Antibody Formation - drug effects</topic><topic>Catalase - blood</topic><topic>Catalase - immunology</topic><topic>Cattle</topic><topic>Complement Fixation Tests</topic><topic>Hot Temperature</topic><topic>Hydrogen-Ion Concentration</topic><topic>Immunodiffusion</topic><topic>Liver</topic><topic>Mice</topic><topic>Molecular Weight</topic><topic>Peptide Hydrolases</topic><topic>Polyethylene Glycols - pharmacology</topic><topic>Rabbits</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Abuchowski, A</creatorcontrib><creatorcontrib>McCoy, J R</creatorcontrib><creatorcontrib>Palczuk, N C</creatorcontrib><creatorcontrib>van Es, T</creatorcontrib><creatorcontrib>Davis, F F</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Abuchowski, A</au><au>McCoy, J R</au><au>Palczuk, N C</au><au>van Es, T</au><au>Davis, F F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of covalent attachment of polyethylene glycol on immunogenicity and circulating life of bovine liver catalase</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1977-06-10</date><risdate>1977</risdate><volume>252</volume><issue>11</issue><spage>3582</spage><epage>3586</epage><pages>3582-3586</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><abstract>Methoxypolyethylene glycols of 1900 daltons (PEG-1900) or 5000 daltons (PEG-5000) were covalently attached to bovine liver
catalase using 2,4,6-trichloro-s-triazine as the coupling agent. Rabbits were immunized by the intravenous and intramuscular
routes with catalase modified by covalent attachment of PEG-1900 to 43% of the amino groups (PEG-1900-catalase). The intravenous
antiserum did not yield detectable antibodies against PEG-1900-catalase or native catalase, as determined by Ouchterlony and
complement fixation methods, whereas the intramuscular antiserum contained antibodies to both PEG-1900-catalase and catalase.
PEG-1900 did not react with either antiserum. Catalase was prepared in which PEG-5000 was attached to 40% of the amino groups
(PEG-5000-catalase). This catalase preparation did not react with either antiserum. PEG-1900-catalase retained 93% of its
enzymatic activity; PEG-5000-catalase retained 95%. PEG-5000-catalase resisted digestion by trypsin, chymotrypsin, and a protease
from Streptomyces griseus. PEG-1900-catalase and PEG-5000-catalase exhibited enhanced circulating lives in the blood of acatalasemic
mice during repetitive intravenous injections. No evidence was seen of an immune response to injections of the modified enzymes.
Mice injected repetitively with PEG-5000-catalase remained immune competent for unmodieied catalase, and no evidence of tissue
or organ damage was seen.</abstract><cop>United States</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>16907</pmid><doi>10.1016/s0021-9258(17)40292-4</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of biological chemistry, 1977-06, Vol.252 (11), p.3582-3586 |
| issn | 0021-9258 1083-351X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_83964527 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Acatalasia Animals Antibody Formation - drug effects Catalase - blood Catalase - immunology Cattle Complement Fixation Tests Hot Temperature Hydrogen-Ion Concentration Immunodiffusion Liver Mice Molecular Weight Peptide Hydrolases Polyethylene Glycols - pharmacology Rabbits |
| title | Effect of covalent attachment of polyethylene glycol on immunogenicity and circulating life of bovine liver catalase |
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