Neospora caninum: Cloning and expression of a gene coding for cytokine-inducing profilin
Profilins are actin-binding proteins that in Toxoplasma gondii stimulate innate immunity in mice by binding Toll-like receptors (TLR) on dendritic cells (DC) leading to release of inflammatory cytokines, primarily IL-12 and IFN-γ. The purpose of the present study was to characterize Neospora caninum...
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| Veröffentlicht in: | Experimental parasitology 2010-08, Vol.125 (4), p.357-362 |
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| creator | Jenkins, Mark C. Tuo, Wenbin Feng, Xiaosheng Cao, Lili Murphy, Charles Fetterer, Raymond |
| description | Profilins are actin-binding proteins that in
Toxoplasma gondii stimulate innate immunity in mice by binding Toll-like receptors (TLR) on dendritic cells (DC) leading to release of inflammatory cytokines, primarily IL-12 and IFN-γ. The purpose of the present study was to characterize
Neospora caninum profilin, termed NcProfilin. Recombinant NcProfilin was purified by affinity chromatography, and used to prepare specific antisera to allow characterization of native NcProfilin antigen in
N. caninum tachyzoites. By immunoblotting, recombinant NcProfilin is 22
kDa, and is similar in size to the respective 22
kDa native protein. Immunofluorescence and immunoelectron microscopy localized native NcProfilin to the apical end of
N. caninum tachyzoites. Incubation of recombinant NcProfilin with spleen cells from BALB/c mice induced release of IFN-γ. Also, injection of BALB/c mice with purified rNcProfilin elicited a strong IFN-γ and IL-12 responses at 6 and 24
h after injection indicating that NcProfilin may be an important protein in regulation of cytokine responses to
N. caninum. |
| doi_str_mv | 10.1016/j.exppara.2010.03.001 |
| format | Article |
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Toxoplasma gondii stimulate innate immunity in mice by binding Toll-like receptors (TLR) on dendritic cells (DC) leading to release of inflammatory cytokines, primarily IL-12 and IFN-γ. The purpose of the present study was to characterize
Neospora caninum profilin, termed NcProfilin. Recombinant NcProfilin was purified by affinity chromatography, and used to prepare specific antisera to allow characterization of native NcProfilin antigen in
N. caninum tachyzoites. By immunoblotting, recombinant NcProfilin is 22
kDa, and is similar in size to the respective 22
kDa native protein. Immunofluorescence and immunoelectron microscopy localized native NcProfilin to the apical end of
N. caninum tachyzoites. Incubation of recombinant NcProfilin with spleen cells from BALB/c mice induced release of IFN-γ. Also, injection of BALB/c mice with purified rNcProfilin elicited a strong IFN-γ and IL-12 responses at 6 and 24
h after injection indicating that NcProfilin may be an important protein in regulation of cytokine responses to
N. caninum.</description><identifier>ISSN: 0014-4894</identifier><identifier>EISSN: 1090-2449</identifier><identifier>DOI: 10.1016/j.exppara.2010.03.001</identifier><identifier>PMID: 20211619</identifier><identifier>CODEN: EXPAAA</identifier><language>eng</language><publisher>Amsterdam: Elsevier Inc</publisher><subject>Affinity chromatography ; Amino Acid Sequence ; Animals ; Base Sequence ; binding proteins ; Biological and medical sciences ; Chromatography, Affinity ; Cloning, Molecular ; Cytokine response ; DNA, Complementary - chemistry ; DNA, Protozoan - chemistry ; Electrophoresis, Polyacrylamide Gel ; Enzyme-Linked Immunosorbent Assay ; Fluorescent Antibody Technique, Indirect ; Fundamental and applied biological sciences. Psychology ; gene expression ; Gene Expression Regulation ; genes ; host-parasite relationships ; immune response ; Interferon-gamma - biosynthesis ; interferons ; interleukin-12 ; Interleukin-12 - biosynthesis ; Life cycle. Host-agent relationship. Pathogenesis ; Mice ; Mice, Inbred BALB C ; microbial proteins ; Microscopy, Immunoelectron ; molecular cloning ; Molecular Sequence Data ; Neospora - genetics ; Neospora - immunology ; Neospora caninum ; Profilin ; Profilins - biosynthesis ; Profilins - chemistry ; Profilins - genetics ; Profilins - immunology ; Protozoa ; Recombinant Proteins - biosynthesis ; Recombinant Proteins - genetics ; Recombinant Proteins - immunology ; Sequence Alignment ; spleen ; Spleen - cytology ; Spleen - immunology ; Tachyzoites</subject><ispartof>Experimental parasitology, 2010-08, Vol.125 (4), p.357-362</ispartof><rights>2010</rights><rights>2015 INIST-CNRS</rights><rights>Published by Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c516t-661bc7b389dbd1e326865f5c9699d4910dcb5c5d2ce5e2379e9440cf3ae962a3</citedby><cites>FETCH-LOGICAL-c516t-661bc7b389dbd1e326865f5c9699d4910dcb5c5d2ce5e2379e9440cf3ae962a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.exppara.2010.03.001$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23428421$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20211619$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jenkins, Mark C.</creatorcontrib><creatorcontrib>Tuo, Wenbin</creatorcontrib><creatorcontrib>Feng, Xiaosheng</creatorcontrib><creatorcontrib>Cao, Lili</creatorcontrib><creatorcontrib>Murphy, Charles</creatorcontrib><creatorcontrib>Fetterer, Raymond</creatorcontrib><title>Neospora caninum: Cloning and expression of a gene coding for cytokine-inducing profilin</title><title>Experimental parasitology</title><addtitle>Exp Parasitol</addtitle><description>Profilins are actin-binding proteins that in
Toxoplasma gondii stimulate innate immunity in mice by binding Toll-like receptors (TLR) on dendritic cells (DC) leading to release of inflammatory cytokines, primarily IL-12 and IFN-γ. The purpose of the present study was to characterize
Neospora caninum profilin, termed NcProfilin. Recombinant NcProfilin was purified by affinity chromatography, and used to prepare specific antisera to allow characterization of native NcProfilin antigen in
N. caninum tachyzoites. By immunoblotting, recombinant NcProfilin is 22
kDa, and is similar in size to the respective 22
kDa native protein. Immunofluorescence and immunoelectron microscopy localized native NcProfilin to the apical end of
N. caninum tachyzoites. Incubation of recombinant NcProfilin with spleen cells from BALB/c mice induced release of IFN-γ. Also, injection of BALB/c mice with purified rNcProfilin elicited a strong IFN-γ and IL-12 responses at 6 and 24
h after injection indicating that NcProfilin may be an important protein in regulation of cytokine responses to
N. caninum.</description><subject>Affinity chromatography</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>binding proteins</subject><subject>Biological and medical sciences</subject><subject>Chromatography, Affinity</subject><subject>Cloning, Molecular</subject><subject>Cytokine response</subject><subject>DNA, Complementary - chemistry</subject><subject>DNA, Protozoan - chemistry</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Fluorescent Antibody Technique, Indirect</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene expression</subject><subject>Gene Expression Regulation</subject><subject>genes</subject><subject>host-parasite relationships</subject><subject>immune response</subject><subject>Interferon-gamma - biosynthesis</subject><subject>interferons</subject><subject>interleukin-12</subject><subject>Interleukin-12 - biosynthesis</subject><subject>Life cycle. Host-agent relationship. Pathogenesis</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>microbial proteins</subject><subject>Microscopy, Immunoelectron</subject><subject>molecular cloning</subject><subject>Molecular Sequence Data</subject><subject>Neospora - genetics</subject><subject>Neospora - immunology</subject><subject>Neospora caninum</subject><subject>Profilin</subject><subject>Profilins - biosynthesis</subject><subject>Profilins - chemistry</subject><subject>Profilins - genetics</subject><subject>Profilins - immunology</subject><subject>Protozoa</subject><subject>Recombinant Proteins - biosynthesis</subject><subject>Recombinant Proteins - genetics</subject><subject>Recombinant Proteins - immunology</subject><subject>Sequence Alignment</subject><subject>spleen</subject><subject>Spleen - cytology</subject><subject>Spleen - immunology</subject><subject>Tachyzoites</subject><issn>0014-4894</issn><issn>1090-2449</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE9vFCEYh4nR2G31I6hcGk-z5d8wgxdjNlZNmnqwJt4IAy8b1lkYYce0314mu-qxJ8iP531_5EHoFSVrSqi82q3hfppMNmtGakb4mhD6BK0oUaRhQqinaFUT0YheiTN0XsqOENJTJp6jM0YYpZKqFfpxC6lMKRtsTQxx3r_DmzHV2xab6HDtyFBKSBEnjw3eQgRsk1vefcrYPhzSzxChCdHNdkmnnHwYQ3yBnnkzFnh5Oi_Q3fXHu83n5ubrpy-bDzeNbak8NFLSwXYD75UbHAXOZC9b31ollXJCUeLs0NrWMQstMN4pUEIQ67kBJZnhF-jtcW3t_TVDOeh9KBbG0URIc9E9V7IubcmjZMc5ZZzzhWyPpM2plAxeTznsTX7QlOhFvt7pk3y9yNeE66q6zr0-NczDHty_qb-2K3B5AkyxZvTZRBvKf44L1gu2LHpz5LxJ2mxzZb5_q02c0L6jbbcQ748EVLW_A2RdbIBowYUM9qBdCo989g_eoK4s</recordid><startdate>20100801</startdate><enddate>20100801</enddate><creator>Jenkins, Mark C.</creator><creator>Tuo, Wenbin</creator><creator>Feng, Xiaosheng</creator><creator>Cao, Lili</creator><creator>Murphy, Charles</creator><creator>Fetterer, Raymond</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T5</scope><scope>H94</scope><scope>M7N</scope></search><sort><creationdate>20100801</creationdate><title>Neospora caninum: Cloning and expression of a gene coding for cytokine-inducing profilin</title><author>Jenkins, Mark C. ; Tuo, Wenbin ; Feng, Xiaosheng ; Cao, Lili ; Murphy, Charles ; Fetterer, Raymond</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c516t-661bc7b389dbd1e326865f5c9699d4910dcb5c5d2ce5e2379e9440cf3ae962a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Affinity chromatography</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>binding proteins</topic><topic>Biological and medical sciences</topic><topic>Chromatography, Affinity</topic><topic>Cloning, Molecular</topic><topic>Cytokine response</topic><topic>DNA, Complementary - chemistry</topic><topic>DNA, Protozoan - chemistry</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Fluorescent Antibody Technique, Indirect</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gene expression</topic><topic>Gene Expression Regulation</topic><topic>genes</topic><topic>host-parasite relationships</topic><topic>immune response</topic><topic>Interferon-gamma - biosynthesis</topic><topic>interferons</topic><topic>interleukin-12</topic><topic>Interleukin-12 - biosynthesis</topic><topic>Life cycle. Host-agent relationship. Pathogenesis</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>microbial proteins</topic><topic>Microscopy, Immunoelectron</topic><topic>molecular cloning</topic><topic>Molecular Sequence Data</topic><topic>Neospora - genetics</topic><topic>Neospora - immunology</topic><topic>Neospora caninum</topic><topic>Profilin</topic><topic>Profilins - biosynthesis</topic><topic>Profilins - chemistry</topic><topic>Profilins - genetics</topic><topic>Profilins - immunology</topic><topic>Protozoa</topic><topic>Recombinant Proteins - biosynthesis</topic><topic>Recombinant Proteins - genetics</topic><topic>Recombinant Proteins - immunology</topic><topic>Sequence Alignment</topic><topic>spleen</topic><topic>Spleen - cytology</topic><topic>Spleen - immunology</topic><topic>Tachyzoites</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jenkins, Mark C.</creatorcontrib><creatorcontrib>Tuo, Wenbin</creatorcontrib><creatorcontrib>Feng, Xiaosheng</creatorcontrib><creatorcontrib>Cao, Lili</creatorcontrib><creatorcontrib>Murphy, Charles</creatorcontrib><creatorcontrib>Fetterer, Raymond</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Experimental parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jenkins, Mark C.</au><au>Tuo, Wenbin</au><au>Feng, Xiaosheng</au><au>Cao, Lili</au><au>Murphy, Charles</au><au>Fetterer, Raymond</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Neospora caninum: Cloning and expression of a gene coding for cytokine-inducing profilin</atitle><jtitle>Experimental parasitology</jtitle><addtitle>Exp Parasitol</addtitle><date>2010-08-01</date><risdate>2010</risdate><volume>125</volume><issue>4</issue><spage>357</spage><epage>362</epage><pages>357-362</pages><issn>0014-4894</issn><eissn>1090-2449</eissn><coden>EXPAAA</coden><abstract>Profilins are actin-binding proteins that in
Toxoplasma gondii stimulate innate immunity in mice by binding Toll-like receptors (TLR) on dendritic cells (DC) leading to release of inflammatory cytokines, primarily IL-12 and IFN-γ. The purpose of the present study was to characterize
Neospora caninum profilin, termed NcProfilin. Recombinant NcProfilin was purified by affinity chromatography, and used to prepare specific antisera to allow characterization of native NcProfilin antigen in
N. caninum tachyzoites. By immunoblotting, recombinant NcProfilin is 22
kDa, and is similar in size to the respective 22
kDa native protein. Immunofluorescence and immunoelectron microscopy localized native NcProfilin to the apical end of
N. caninum tachyzoites. Incubation of recombinant NcProfilin with spleen cells from BALB/c mice induced release of IFN-γ. Also, injection of BALB/c mice with purified rNcProfilin elicited a strong IFN-γ and IL-12 responses at 6 and 24
h after injection indicating that NcProfilin may be an important protein in regulation of cytokine responses to
N. caninum.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><pmid>20211619</pmid><doi>10.1016/j.exppara.2010.03.001</doi><tpages>6</tpages></addata></record> |
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| ispartof | Experimental parasitology, 2010-08, Vol.125 (4), p.357-362 |
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| subjects | Affinity chromatography Amino Acid Sequence Animals Base Sequence binding proteins Biological and medical sciences Chromatography, Affinity Cloning, Molecular Cytokine response DNA, Complementary - chemistry DNA, Protozoan - chemistry Electrophoresis, Polyacrylamide Gel Enzyme-Linked Immunosorbent Assay Fluorescent Antibody Technique, Indirect Fundamental and applied biological sciences. Psychology gene expression Gene Expression Regulation genes host-parasite relationships immune response Interferon-gamma - biosynthesis interferons interleukin-12 Interleukin-12 - biosynthesis Life cycle. Host-agent relationship. Pathogenesis Mice Mice, Inbred BALB C microbial proteins Microscopy, Immunoelectron molecular cloning Molecular Sequence Data Neospora - genetics Neospora - immunology Neospora caninum Profilin Profilins - biosynthesis Profilins - chemistry Profilins - genetics Profilins - immunology Protozoa Recombinant Proteins - biosynthesis Recombinant Proteins - genetics Recombinant Proteins - immunology Sequence Alignment spleen Spleen - cytology Spleen - immunology Tachyzoites |
| title | Neospora caninum: Cloning and expression of a gene coding for cytokine-inducing profilin |
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