GLC Determination of Acetohexamide and Hydroxyhexamide in Biological Fluids
A sensitive and specific GLC assay was developed for acetohexamide and hydroxyhexamide, its major metabolite, in plasma and urine. The assay uses tolbutamide as a mass internal standard. Compounds are extracted from acidified plasma or urine with toluene, converted to methylated derivatives with dim...
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| Veröffentlicht in: | Journal of pharmaceutical sciences 1977-05, Vol.66 (5), p.635-638 |
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| container_end_page | 638 |
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| container_issue | 5 |
| container_start_page | 635 |
| container_title | Journal of pharmaceutical sciences |
| container_volume | 66 |
| creator | Kleber, J.W. Galloway, J.A. Rodda, B.E. |
| description | A sensitive and specific GLC assay was developed for acetohexamide and hydroxyhexamide, its major metabolite, in plasma and urine. The assay uses tolbutamide as a mass internal standard. Compounds are extracted from acidified plasma or urine with toluene, converted to methylated derivatives with dimethyl sulfate, and measured by GLC using a flame-ionization detector. With GLC-mass spectrometry, the compounds measured are the N-methylsulfonamides resulting from GLC pyrolysis. Plasma and urine data are presented from a bioavailability study to demonstrate the utility of this method. |
| doi_str_mv | 10.1002/jps.2600660507 |
| format | Article |
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The assay uses tolbutamide as a mass internal standard. Compounds are extracted from acidified plasma or urine with toluene, converted to methylated derivatives with dimethyl sulfate, and measured by GLC using a flame-ionization detector. With GLC-mass spectrometry, the compounds measured are the N-methylsulfonamides resulting from GLC pyrolysis. 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Pharm. Sci</addtitle><description>A sensitive and specific GLC assay was developed for acetohexamide and hydroxyhexamide, its major metabolite, in plasma and urine. The assay uses tolbutamide as a mass internal standard. Compounds are extracted from acidified plasma or urine with toluene, converted to methylated derivatives with dimethyl sulfate, and measured by GLC using a flame-ionization detector. With GLC-mass spectrometry, the compounds measured are the N-methylsulfonamides resulting from GLC pyrolysis. Plasma and urine data are presented from a bioavailability study to demonstrate the utility of this method.</description><subject>Acetohexamide - analogs & derivatives</subject><subject>Acetohexamide - analysis</subject><subject>Acetohexamide - blood</subject><subject>Acetohexamide - urine</subject><subject>acetohexamide and hydroxyhexamide</subject><subject>Acetohexamide-GLC analysis</subject><subject>Acetohexamide—GLC analysis, human plasma and urine</subject><subject>Antidiabetic agents-acetohexamide</subject><subject>Antidiabetic agents—acetohexamide, GLC analysis, human plasma and urine</subject><subject>Biological Availability</subject><subject>Blood Glucose - analysis</subject><subject>Chromatography, Gas</subject><subject>GLC analysis</subject><subject>GLC-analyses</subject><subject>GLC—analyses, acetohexamide and hydroxyhexamide, human plasma and urine</subject><subject>human plasma and urine</subject><subject>Humans</subject><subject>Hydroxyhexamide-GLC analysis</subject><subject>Hydroxyhexamide—GLC analysis, human plasma and urine</subject><subject>Insulin - blood</subject><subject>Mass Spectrometry</subject><subject>Methods</subject><subject>Tablets</subject><subject>Time Factors</subject><issn>0022-3549</issn><issn>1520-6017</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1977</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEFv0zAYhi0Eg7Jx5bRDTtxS7Di24-MotAUqQGLTjpZjf948krjYKbT_Hk-ZOnFAnCz5e97Hn1-EXhM8JxhXb--2aV5xjDnHDIsnaEZYhUuOiXiKZhmoSspq-QK9TOkOZwwz9hydNKIWNZ2hz6vNongPI8TeD3r0YSiCKy4MjOEW9rr3Fgo92GJ9sDHsD8c7PxTvfOjCjTe6K5bdztt0hp453SV49XCeoqvlh8vFutx8XX1cXGxKU1dclHlL4oAJS2hNtXTEYKuBtQ04R1quBYGWNrQFaZwgnEpBGDcNGGdkxQylp-jN5N3G8HMHaVS9Twa6Tg8Qdkk1VNZVI0UG5xNoYkgpglPb6HsdD4pgdV-eyuWpx_Jy4PzBvGt7sEd8aiuP5TT-7Ts4_EemPn37_pe6nLI-jbA_ZnX8obiggqnrLyu15s31Umbf_VvNxENu8peHqJLxMBiwPoIZlQ3-X7_4A5LKnMM</recordid><startdate>197705</startdate><enddate>197705</enddate><creator>Kleber, J.W.</creator><creator>Galloway, J.A.</creator><creator>Rodda, B.E.</creator><general>Elsevier Inc</general><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197705</creationdate><title>GLC Determination of Acetohexamide and Hydroxyhexamide in Biological Fluids</title><author>Kleber, J.W. ; Galloway, J.A. ; Rodda, B.E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4267-5071fe57d1343a9f1c0dae5b8eff1b6a71eb383be9cf716397156c8ecfc925c33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1977</creationdate><topic>Acetohexamide - analogs & derivatives</topic><topic>Acetohexamide - analysis</topic><topic>Acetohexamide - blood</topic><topic>Acetohexamide - urine</topic><topic>acetohexamide and hydroxyhexamide</topic><topic>Acetohexamide-GLC analysis</topic><topic>Acetohexamide—GLC analysis, human plasma and urine</topic><topic>Antidiabetic agents-acetohexamide</topic><topic>Antidiabetic agents—acetohexamide, GLC analysis, human plasma and urine</topic><topic>Biological Availability</topic><topic>Blood Glucose - analysis</topic><topic>Chromatography, Gas</topic><topic>GLC analysis</topic><topic>GLC-analyses</topic><topic>GLC—analyses, acetohexamide and hydroxyhexamide, human plasma and urine</topic><topic>human plasma and urine</topic><topic>Humans</topic><topic>Hydroxyhexamide-GLC analysis</topic><topic>Hydroxyhexamide—GLC analysis, human plasma and urine</topic><topic>Insulin - blood</topic><topic>Mass Spectrometry</topic><topic>Methods</topic><topic>Tablets</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kleber, J.W.</creatorcontrib><creatorcontrib>Galloway, J.A.</creatorcontrib><creatorcontrib>Rodda, B.E.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of pharmaceutical sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kleber, J.W.</au><au>Galloway, J.A.</au><au>Rodda, B.E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>GLC Determination of Acetohexamide and Hydroxyhexamide in Biological Fluids</atitle><jtitle>Journal of pharmaceutical sciences</jtitle><addtitle>J. Pharm. Sci</addtitle><date>1977-05</date><risdate>1977</risdate><volume>66</volume><issue>5</issue><spage>635</spage><epage>638</epage><pages>635-638</pages><issn>0022-3549</issn><eissn>1520-6017</eissn><abstract>A sensitive and specific GLC assay was developed for acetohexamide and hydroxyhexamide, its major metabolite, in plasma and urine. The assay uses tolbutamide as a mass internal standard. Compounds are extracted from acidified plasma or urine with toluene, converted to methylated derivatives with dimethyl sulfate, and measured by GLC using a flame-ionization detector. With GLC-mass spectrometry, the compounds measured are the N-methylsulfonamides resulting from GLC pyrolysis. Plasma and urine data are presented from a bioavailability study to demonstrate the utility of this method.</abstract><cop>Washington</cop><pub>Elsevier Inc</pub><pmid>874743</pmid><doi>10.1002/jps.2600660507</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-3549 |
| ispartof | Journal of pharmaceutical sciences, 1977-05, Vol.66 (5), p.635-638 |
| issn | 0022-3549 1520-6017 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_83942897 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Alma/SFX Local Collection |
| subjects | Acetohexamide - analogs & derivatives Acetohexamide - analysis Acetohexamide - blood Acetohexamide - urine acetohexamide and hydroxyhexamide Acetohexamide-GLC analysis Acetohexamide—GLC analysis, human plasma and urine Antidiabetic agents-acetohexamide Antidiabetic agents—acetohexamide, GLC analysis, human plasma and urine Biological Availability Blood Glucose - analysis Chromatography, Gas GLC analysis GLC-analyses GLC—analyses, acetohexamide and hydroxyhexamide, human plasma and urine human plasma and urine Humans Hydroxyhexamide-GLC analysis Hydroxyhexamide—GLC analysis, human plasma and urine Insulin - blood Mass Spectrometry Methods Tablets Time Factors |
| title | GLC Determination of Acetohexamide and Hydroxyhexamide in Biological Fluids |
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