Separation of isoacceptor cysteine transfer ribonucleic acids of bakers' yeasts
Isoacceptor species of certain amino acid-specific transfer ribonucleic acids (tRNAs) were fractionated by gel permeation chromatography using Sephadex G-100. The separation is attributed to the 20% ethanol-1% NaCl solvent and to the characteristics of Sephadex. Isoacceptor tRNAs specific for cystei...
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| Veröffentlicht in: | Molecular and cellular biochemistry 1977-03, Vol.15 (1), p.15-17 |
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| container_title | Molecular and cellular biochemistry |
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| creator | James, H.L Morrison, J.C Whybrew, W.D Trass, T.C Bucovaz, E.T |
| description | Isoacceptor species of certain amino acid-specific transfer ribonucleic acids (tRNAs) were fractionated by gel permeation chromatography using Sephadex G-100. The separation is attributed to the 20% ethanol-1% NaCl solvent and to the characteristics of Sephadex. Isoacceptor tRNAs specific for cysteine, arginine, phenylalanine, and histidine were recovered from commercial tRNA of yeast by this method. Highly purified cysteine-specific tRNA, obtained by a method which would not be expected to separate isoacceptor molecules when fractionated by this procedure, was shown to contain two cysteine isoacceptor tRNAs. |
| doi_str_mv | 10.1007/BF01731285 |
| format | Article |
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Highly purified cysteine-specific tRNA, obtained by a method which would not be expected to separate isoacceptor molecules when fractionated by this procedure, was shown to contain two cysteine isoacceptor tRNAs.</description><identifier>ISSN: 0300-8177</identifier><identifier>EISSN: 1573-4919</identifier><identifier>DOI: 10.1007/BF01731285</identifier><identifier>PMID: 325392</identifier><language>eng</language><publisher>Netherlands</publisher><subject>Amino Acyl-tRNA Synthetases - metabolism ; Chromatography, Gel ; Cysteine ; plant biochemistry ; plant physiology ; RNA, Transfer - isolation & purification ; Saccharomyces cerevisiae - analysis ; Saccharomyces cerevisiae - enzymology</subject><ispartof>Molecular and cellular biochemistry, 1977-03, Vol.15 (1), p.15-17</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c264t-3665fc67917430c201ccf4bf44d9ccafd98f0f05f3ab0b5c6f606cc080702e163</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/325392$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>James, H.L</creatorcontrib><creatorcontrib>Morrison, J.C</creatorcontrib><creatorcontrib>Whybrew, W.D</creatorcontrib><creatorcontrib>Trass, T.C</creatorcontrib><creatorcontrib>Bucovaz, E.T</creatorcontrib><title>Separation of isoacceptor cysteine transfer ribonucleic acids of bakers' yeasts</title><title>Molecular and cellular biochemistry</title><addtitle>Mol Cell Biochem</addtitle><description>Isoacceptor species of certain amino acid-specific transfer ribonucleic acids (tRNAs) were fractionated by gel permeation chromatography using Sephadex G-100. The separation is attributed to the 20% ethanol-1% NaCl solvent and to the characteristics of Sephadex. Isoacceptor tRNAs specific for cysteine, arginine, phenylalanine, and histidine were recovered from commercial tRNA of yeast by this method. Highly purified cysteine-specific tRNA, obtained by a method which would not be expected to separate isoacceptor molecules when fractionated by this procedure, was shown to contain two cysteine isoacceptor tRNAs.</description><subject>Amino Acyl-tRNA Synthetases - metabolism</subject><subject>Chromatography, Gel</subject><subject>Cysteine</subject><subject>plant biochemistry</subject><subject>plant physiology</subject><subject>RNA, Transfer - isolation & purification</subject><subject>Saccharomyces cerevisiae - analysis</subject><subject>Saccharomyces cerevisiae - enzymology</subject><issn>0300-8177</issn><issn>1573-4919</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1977</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkD1PwzAURS3EVykszEhkAgkp8GwndjxCRQGpUofSOXJebGRI42InQ_89qYJgesM99-rpEHJJ4Z4CyIenOVDJKSvyAzKhueRppqg6JBPgAGlBpTwlZzF-Agw4pSfkmLOcKzYhy5XZ6qA759vE28RFrxHNtvMhwV3sjGtN0gXdRmtCElzl2x4b4zDR6Oq4r1T6y4R4m-yMjl08J0dWN9Fc_N4pWc-f32ev6WL58jZ7XKTIRNalXIjcopCKyowDMqCINqtsltUKUdtaFRYs5JbrCqochRUgEKEACcxQwafkZtzdBv_dm9iVGxfRNI1uje9jWXDFRc7YAN6NIAYfYzC23Aa30WFXUij38sp_eQN89bvaVxtT_6GjrSG-HmOrfak_govlejX8zoEpmYsM-A-Ap3Ki</recordid><startdate>19770321</startdate><enddate>19770321</enddate><creator>James, H.L</creator><creator>Morrison, J.C</creator><creator>Whybrew, W.D</creator><creator>Trass, T.C</creator><creator>Bucovaz, E.T</creator><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19770321</creationdate><title>Separation of isoacceptor cysteine transfer ribonucleic acids of bakers' yeasts</title><author>James, H.L ; Morrison, J.C ; Whybrew, W.D ; Trass, T.C ; Bucovaz, E.T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c264t-3665fc67917430c201ccf4bf44d9ccafd98f0f05f3ab0b5c6f606cc080702e163</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1977</creationdate><topic>Amino Acyl-tRNA Synthetases - metabolism</topic><topic>Chromatography, Gel</topic><topic>Cysteine</topic><topic>plant biochemistry</topic><topic>plant physiology</topic><topic>RNA, Transfer - isolation & purification</topic><topic>Saccharomyces cerevisiae - analysis</topic><topic>Saccharomyces cerevisiae - enzymology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>James, H.L</creatorcontrib><creatorcontrib>Morrison, J.C</creatorcontrib><creatorcontrib>Whybrew, W.D</creatorcontrib><creatorcontrib>Trass, T.C</creatorcontrib><creatorcontrib>Bucovaz, E.T</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular and cellular biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>James, H.L</au><au>Morrison, J.C</au><au>Whybrew, W.D</au><au>Trass, T.C</au><au>Bucovaz, E.T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Separation of isoacceptor cysteine transfer ribonucleic acids of bakers' yeasts</atitle><jtitle>Molecular and cellular biochemistry</jtitle><addtitle>Mol Cell Biochem</addtitle><date>1977-03-21</date><risdate>1977</risdate><volume>15</volume><issue>1</issue><spage>15</spage><epage>17</epage><pages>15-17</pages><issn>0300-8177</issn><eissn>1573-4919</eissn><abstract>Isoacceptor species of certain amino acid-specific transfer ribonucleic acids (tRNAs) were fractionated by gel permeation chromatography using Sephadex G-100. 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| ispartof | Molecular and cellular biochemistry, 1977-03, Vol.15 (1), p.15-17 |
| issn | 0300-8177 1573-4919 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Amino Acyl-tRNA Synthetases - metabolism Chromatography, Gel Cysteine plant biochemistry plant physiology RNA, Transfer - isolation & purification Saccharomyces cerevisiae - analysis Saccharomyces cerevisiae - enzymology |
| title | Separation of isoacceptor cysteine transfer ribonucleic acids of bakers' yeasts |
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