Rapid Gas Chromatographic Separation of Amino Acid Enantiomers with a Novel Chiral Stationary Phase

Rapid Gas Chromatographic Separation of Amino Acid Enantiomers with a Novel Chiral Stationary Phase

The use of novel polysiloxanes as stationary phase carrying chiral groups enables the separation of most amino acid enantiomers in a much shorter time than ever reported previously. Phases of this type exhibit very low volatility and high thermal stability and may be used in routine analysis with op...

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Veröffentlicht in:Journal of chromatographic science 1977-05, Vol.15 (5), p.174-176
Hauptverfasser: Frank, Hartmut, Nicholson, Graeme J., Bayer, Ernst
Format: Artikel
Sprache:eng
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Amino Acids - analysis
Chromatography, Gas - methods
Drug Stability
Isomerism
Siloxanes
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container_end_page 176
container_issue 5
container_start_page 174
container_title Journal of chromatographic science
container_volume 15
creator Frank, Hartmut
Nicholson, Graeme J.
Bayer, Ernst
description The use of novel polysiloxanes as stationary phase carrying chiral groups enables the separation of most amino acid enantiomers in a much shorter time than ever reported previously. Phases of this type exhibit very low volatility and high thermal stability and may be used in routine analysis with open tubular columns at temperatures of at least 175°C. Most protein amino acids are separated in a temperature program between 90 and 175°C, thus obviating the need for multiple injections. Resolution factors are somewhat lower than those of other diamide phases containing the L-valine t-butylamide group, but are sufficient for resolution of almost all protein amino acid enantiomers.
doi_str_mv 10.1093/chromsci/15.5.174
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ispartof Journal of chromatographic science, 1977-05, Vol.15 (5), p.174-176
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language eng
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source Oxford University Press Journals Digital Archive legacy; MEDLINE
subjects Amino Acids - analysis
Chromatography, Gas - methods
Drug Stability
Isomerism
Siloxanes
title Rapid Gas Chromatographic Separation of Amino Acid Enantiomers with a Novel Chiral Stationary Phase
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