A new serum α-amylase assay of high sensitivity
The use of chemically modified starch substrates for measurement of serum α-amylase activity is described. Action of α-amylase on such substrates, in the presence of excess fungal glucoamylase, results in the production of glucose in direct proportion to the amount of α-amylase present. The glucose...
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| Veröffentlicht in: | Clinica chimica acta 1977-04, Vol.76 (2), p.277-283 |
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| container_title | Clinica chimica acta |
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| creator | Marshall, J.John Iodice, Arthur P. Whelan, William J. |
| description | The use of chemically modified starch substrates for measurement of serum α-amylase activity is described. Action of α-amylase on such substrates, in the presence of excess fungal glucoamylase, results in the production of glucose in direct proportion to the amount of α-amylase present. The glucose produced is measured by a specific enzymic assay. Results obtained by using this new assay correlate well with activities determined by a conventional saccharogenic assay. The new method is of much higher sensitivity, and is less susceptible to interference, than are most other α-amylase assay methods. |
| doi_str_mv | 10.1016/0009-8981(77)90107-3 |
| format | Article |
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Action of α-amylase on such substrates, in the presence of excess fungal glucoamylase, results in the production of glucose in direct proportion to the amount of α-amylase present. The glucose produced is measured by a specific enzymic assay. Results obtained by using this new assay correlate well with activities determined by a conventional saccharogenic assay. The new method is of much higher sensitivity, and is less susceptible to interference, than are most other α-amylase assay methods.</description><identifier>ISSN: 0009-8981</identifier><identifier>EISSN: 1873-3492</identifier><identifier>DOI: 10.1016/0009-8981(77)90107-3</identifier><identifier>PMID: 862201</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Amylases - blood ; Amylases - metabolism ; Amylose - metabolism ; Glucosidases - metabolism ; Hydrolysis ; Methods ; Oxidation-Reduction ; Saliva - enzymology ; Starch - metabolism ; Time Factors</subject><ispartof>Clinica chimica acta, 1977-04, Vol.76 (2), p.277-283</ispartof><rights>1977</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-9b71d4409f38e8393255016aa0d1e7ef7ac5c93054ab0b2bf92a17be05babf23</citedby><cites>FETCH-LOGICAL-c356t-9b71d4409f38e8393255016aa0d1e7ef7ac5c93054ab0b2bf92a17be05babf23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0009898177901073$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/862201$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Marshall, J.John</creatorcontrib><creatorcontrib>Iodice, Arthur P.</creatorcontrib><creatorcontrib>Whelan, William J.</creatorcontrib><title>A new serum α-amylase assay of high sensitivity</title><title>Clinica chimica acta</title><addtitle>Clin Chim Acta</addtitle><description>The use of chemically modified starch substrates for measurement of serum α-amylase activity is described. Action of α-amylase on such substrates, in the presence of excess fungal glucoamylase, results in the production of glucose in direct proportion to the amount of α-amylase present. The glucose produced is measured by a specific enzymic assay. Results obtained by using this new assay correlate well with activities determined by a conventional saccharogenic assay. The new method is of much higher sensitivity, and is less susceptible to interference, than are most other α-amylase assay methods.</description><subject>Amylases - blood</subject><subject>Amylases - metabolism</subject><subject>Amylose - metabolism</subject><subject>Glucosidases - metabolism</subject><subject>Hydrolysis</subject><subject>Methods</subject><subject>Oxidation-Reduction</subject><subject>Saliva - enzymology</subject><subject>Starch - metabolism</subject><subject>Time Factors</subject><issn>0009-8981</issn><issn>1873-3492</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1977</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMlOwzAQhi3EVgpv0ENOCA6BcZzE8QWpqtikSlx6t2xnQo2yFDstymPxIjwTCal65DQa_YtmPkJmFO4o0PQeAESYiYzecH4rgAIP2RGZ0IyzkMUiOiaTg-WcXHj_0a8xpPSMnGZpFAGdEJgHNX4FHt22Cn6-Q1V1pfIYKO9VFzRFsLbv616uvW3tzrbdJTkpVOnxaj-nZPX0uFq8hMu359fFfBkalqRtKDSneRyDKFiGGRMsSpL-ZqUgp8ix4MokRjBIYqVBR7oQkaJcIyRa6SJiU3I91m5c87lF38rKeoNlqWpstl72lZCKiPXGeDQa13jvsJAbZyvlOklBDpjkwEAODCTn8g-THGKzff9WV5gfQiOXXn4YZexf3Fl00huLtcHcOjStzBv7f_8v3RZ2Bg</recordid><startdate>19770415</startdate><enddate>19770415</enddate><creator>Marshall, J.John</creator><creator>Iodice, Arthur P.</creator><creator>Whelan, William J.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19770415</creationdate><title>A new serum α-amylase assay of high sensitivity</title><author>Marshall, J.John ; Iodice, Arthur P. ; Whelan, William J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-9b71d4409f38e8393255016aa0d1e7ef7ac5c93054ab0b2bf92a17be05babf23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1977</creationdate><topic>Amylases - blood</topic><topic>Amylases - metabolism</topic><topic>Amylose - metabolism</topic><topic>Glucosidases - metabolism</topic><topic>Hydrolysis</topic><topic>Methods</topic><topic>Oxidation-Reduction</topic><topic>Saliva - enzymology</topic><topic>Starch - metabolism</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Marshall, J.John</creatorcontrib><creatorcontrib>Iodice, Arthur P.</creatorcontrib><creatorcontrib>Whelan, William J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinica chimica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Marshall, J.John</au><au>Iodice, Arthur P.</au><au>Whelan, William J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A new serum α-amylase assay of high sensitivity</atitle><jtitle>Clinica chimica acta</jtitle><addtitle>Clin Chim Acta</addtitle><date>1977-04-15</date><risdate>1977</risdate><volume>76</volume><issue>2</issue><spage>277</spage><epage>283</epage><pages>277-283</pages><issn>0009-8981</issn><eissn>1873-3492</eissn><abstract>The use of chemically modified starch substrates for measurement of serum α-amylase activity is described. Action of α-amylase on such substrates, in the presence of excess fungal glucoamylase, results in the production of glucose in direct proportion to the amount of α-amylase present. The glucose produced is measured by a specific enzymic assay. Results obtained by using this new assay correlate well with activities determined by a conventional saccharogenic assay. The new method is of much higher sensitivity, and is less susceptible to interference, than are most other α-amylase assay methods.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>862201</pmid><doi>10.1016/0009-8981(77)90107-3</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Clinica chimica acta, 1977-04, Vol.76 (2), p.277-283 |
| issn | 0009-8981 1873-3492 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_83906923 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Amylases - blood Amylases - metabolism Amylose - metabolism Glucosidases - metabolism Hydrolysis Methods Oxidation-Reduction Saliva - enzymology Starch - metabolism Time Factors |
| title | A new serum α-amylase assay of high sensitivity |
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