The effect of various conditions of chromatin isolation on the nucleosomal structure of the isolated chromatin

Chromatin from calf thymus isolated under hypotonic conditions in the presence of various agents was investigated by methods of electron microscopy prior to and after EDTA treatment. It is shown that the presence of chelating agents and, especially, the application of considerable mechanical forces...

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Veröffentlicht in:	Molecular biology reports 1978-02, Vol.4 (1), p.45-50
Hauptverfasser:	Popenko, V I, Vengerov, Y Y
Format:	Artikel
Sprache:	eng
Schlagworte:	Chromatin - drug effects Chromatin - isolation & purification Chromatin - ultrastructure Edetic Acid - pharmacology
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creator	Popenko, V I Vengerov, Y Y
description	Chromatin from calf thymus isolated under hypotonic conditions in the presence of various agents was investigated by methods of electron microscopy prior to and after EDTA treatment. It is shown that the presence of chelating agents and, especially, the application of considerable mechanical forces in the course of isolation may cause damage to the nucleosomal structure of the chromatin. Moreover, sufficiently great mechanical forces are liable to destroy the structure of the chromatin nucleosomal fibres even when they are packed in structures of a higher order of organization of the chromatin.
doi_str_mv	10.1007/BF00775180
format	Article
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Moreover, sufficiently great mechanical forces are liable to destroy the structure of the chromatin nucleosomal fibres even when they are packed in structures of a higher order of organization of the chromatin.</description><identifier>ISSN: 0301-4851</identifier><identifier>EISSN: 1573-4978</identifier><identifier>DOI: 10.1007/BF00775180</identifier><identifier>PMID: 417238</identifier><language>eng</language><publisher>Netherlands</publisher><subject>Chromatin - drug effects ; Chromatin - isolation & purification ; Chromatin - ultrastructure ; Edetic Acid - pharmacology</subject><ispartof>Molecular biology reports, 1978-02, Vol.4 (1), p.45-50</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c281t-add883c11cab853974252953775b2c11b4d838e85868784a3d775ff3529b34f53</citedby><cites>FETCH-LOGICAL-c281t-add883c11cab853974252953775b2c11b4d838e85868784a3d775ff3529b34f53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/417238$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Popenko, V I</creatorcontrib><creatorcontrib>Vengerov, Y Y</creatorcontrib><title>The effect of various conditions of chromatin isolation on the nucleosomal structure of the isolated chromatin</title><title>Molecular biology reports</title><addtitle>Mol Biol Rep</addtitle><description>Chromatin from calf thymus isolated under hypotonic conditions in the presence of various agents was investigated by methods of electron microscopy prior to and after EDTA treatment. 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language	eng
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source	MEDLINE; SpringerNature Journals
subjects	Chromatin - drug effects Chromatin - isolation & purification Chromatin - ultrastructure Edetic Acid - pharmacology
title	The effect of various conditions of chromatin isolation on the nucleosomal structure of the isolated chromatin
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