Molecular Basis of B Cell Activation: Mitogenicity of Native and Modified Digitalis Glycosides
Lanatoside C, a digitalis glycoside, has previously been shown to be a potent polyclonal B cell activator for mouse lymphocytes. Since the chemical structure of lanatoside C is well defined we investigated the effect of different chemical modifications on the mitogenic properties of the glycoside in...
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| Veröffentlicht in: | The Journal of immunology (1950) 1978-03, Vol.120 (3), p.694-699 |
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| container_title | The Journal of immunology (1950) |
| container_volume | 120 |
| creator | Hammarstrom, Lennart L. G Smith, C. I. Edvard |
| description | Lanatoside C, a digitalis glycoside, has previously been shown to be a potent polyclonal B cell activator for mouse lymphocytes. Since the chemical structure of lanatoside C is well defined we investigated the effect of different chemical modifications on the mitogenic properties of the glycoside in order to determine the molecular basis of B cell activation. The presence of an OH- group at C12 in the steroid nucleus proved to be essential for activation to occur in serum-free medium. Likewise, an intact carbohydrate side chain at C3 appeared to be necessary for retaining mitogenic properties. In serum-supplemented cultures, however, a much more complex response pattern was observed where the prerequisites for activation were not as distinct as in serum-free medium. |
| doi_str_mv | 10.4049/jimmunol.120.3.694 |
| format | Article |
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In serum-supplemented cultures, however, a much more complex response pattern was observed where the prerequisites for activation were not as distinct as in serum-free medium.</description><subject>Animals</subject><subject>B-Lymphocytes - immunology</subject><subject>Carbohydrate Metabolism</subject><subject>Carbon - metabolism</subject><subject>Culture Media</subject><subject>Digitalis Glycosides - pharmacology</subject><subject>Female</subject><subject>Lanatosides - pharmacology</subject><subject>Lymphocyte Activation</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred A</subject><subject>Mice, Inbred CBA</subject><subject>Mitogens</subject><issn>0022-1767</issn><issn>1550-6606</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1978</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkMlOwzAURS3EVIYfQCy8YpfgqXbCri2jRGEDWyzXdoqRE0OcEPXvcVWgqyc93Xue3gHgDKOcIVZefri67pvgc0xQTnNesh0wwuMxyjhHfBeMECIkw4KLQ3AU4wdCiCPCDsA-RWPG6Ai8zYO3uveqhVMVXYShglM4s97Die7ct-pcaK7g3HVhaRunXbdaR57S_ttC1Rg4D8ZVzhp47ZauUz4x7vxKh-iMjSdgr1I-2tPfeQxeb29eZvfZ4_Pdw2zymGlKii6zhpUVtQsqiNZKMFOwio9JURJOeIW5thgXHFeKCC1Mel1TpKlaCFQSYxmlx-Biw_1sw1dvYydrF3X6QjU29FEWtBBlQUQKkk1QtyHG1lbys3W1alcSI7l2Kv-cyuRUUpmcptL5L71f1Nb8VzYSt8ff3fJ9cK2VsVbepzCWwzBsOT8EfIHJ</recordid><startdate>197803</startdate><enddate>197803</enddate><creator>Hammarstrom, Lennart L. 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| ispartof | The Journal of immunology (1950), 1978-03, Vol.120 (3), p.694-699 |
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| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals B-Lymphocytes - immunology Carbohydrate Metabolism Carbon - metabolism Culture Media Digitalis Glycosides - pharmacology Female Lanatosides - pharmacology Lymphocyte Activation Male Mice Mice, Inbred A Mice, Inbred CBA Mitogens |
| title | Molecular Basis of B Cell Activation: Mitogenicity of Native and Modified Digitalis Glycosides |
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