A microassay for the peptidase activity of enzymes utilizing ribonuclease S peptide as a substrate

A microassay for the peptidase activity of enzymes utilizing ribonuclease S peptide as a substrate

A microassay for the peptidase activity of proteins obtained in minute amounts was devised. The method uses ribonuclease S peptide as a substrate. The substrate when cleaved is unable to reconstitute an active ribonuclease S complex. Therefore the loss in activity of the reconstituted complex is a m...

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Veröffentlicht in:Analytical biochemistry 1978, Vol.84 (1), p.343-345
Hauptverfasser: Levit, Shimon, Joshi, Madhusudan S.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Female
Male
Microchemistry
Peptide Fragments - metabolism
Peptide Hydrolases - metabolism
Pregnancy
Proestrus
Rats
Ribonucleases - metabolism
Sperm Capacitation
Uterus - enzymology
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container_title Analytical biochemistry
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creator Levit, Shimon
Joshi, Madhusudan S.
description A microassay for the peptidase activity of proteins obtained in minute amounts was devised. The method uses ribonuclease S peptide as a substrate. The substrate when cleaved is unable to reconstitute an active ribonuclease S complex. Therefore the loss in activity of the reconstituted complex is a measure of the peptidase activity. The method was previously tested with known peptidases such as clastase (9), chymotrypsin (8), and trypsin. In this work the peptidase activity of a protein related to a sperm-decapitating factor (1) is evidenced.
doi_str_mv 10.1016/0003-2697(78)90522-5
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source MEDLINE; Elsevier ScienceDirect Journals
subjects Animals
Female
Male
Microchemistry
Peptide Fragments - metabolism
Peptide Hydrolases - metabolism
Pregnancy
Proestrus
Rats
Ribonucleases - metabolism
Sperm Capacitation
Uterus - enzymology
title A microassay for the peptidase activity of enzymes utilizing ribonuclease S peptide as a substrate
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