Preparative immunoabsorption electrophoresis

A method for separating a component from a mixture of antigens is described. The component which may be a virus or subfraction of a virus, is isolated by driving the mixture by electrophoresis through a gel containing precipitating antibodies directed against the unwanted components. The method is i...

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Veröffentlicht in:Immunological communications 1978, Vol.7 (1), p.91-102
Hauptverfasser: Polson, A, von Wechmar, M B, Moodie, J W
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container_title Immunological communications
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creator Polson, A
von Wechmar, M B
Moodie, J W
description A method for separating a component from a mixture of antigens is described. The component which may be a virus or subfraction of a virus, is isolated by driving the mixture by electrophoresis through a gel containing precipitating antibodies directed against the unwanted components. The method is illustrated by the isolation of hepatitis B antigen from whole serum and by the separation of wild cucumber mosaic virus from a strain of tobacco mosaic virus.
doi_str_mv 10.3109/08820137809033926
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source MEDLINE; Taylor & Francis:Master (3349 titles)
subjects Absorption
Animals
Hepatitis B Surface Antigens - isolation & purification
Humans
Immunoelectrophoresis - instrumentation
Immunoelectrophoresis - methods
Mosaic Viruses - isolation & purification
Mosaic Viruses - ultrastructure
Rabbits
title Preparative immunoabsorption electrophoresis
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