Rod-like elements from actin-containing microfilament bundles observed in cultured cells after treatment with cytochalasin A (CA)

Rod-like elements from actin-containing microfilament bundles observed in cultured cells after treatment with cytochalasin A (CA)

Immunofluorescence microscopy using antibody against actin has been used to study the expression of microfilamentous material in cells of a cloned mouse 3T3 line during cytochalasin A (CA) induced cell contraction. A conspicuous modification of the structure of the microfilament bundles is observed....

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Veröffentlicht in:Experimental cell research 1977-03, Vol.105 (2), p.253-262
Hauptverfasser: Rathke, P.C., Seib, Erinita, Weber, K., Osborn, Mary, Franke, W.W.
Format: Artikel
Sprache:eng
Schlagworte:
Actins
Cell Line
Cytochalasins - pharmacology
Cytoplasm - drug effects
Cytoskeleton - drug effects
Cytoskeleton - ultrastructure
Fluorescent Antibody Technique
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container_end_page 262
container_issue 2
container_start_page 253
container_title Experimental cell research
container_volume 105
creator Rathke, P.C.
Seib, Erinita
Weber, K.
Osborn, Mary
Franke, W.W.
description Immunofluorescence microscopy using antibody against actin has been used to study the expression of microfilamentous material in cells of a cloned mouse 3T3 line during cytochalasin A (CA) induced cell contraction. A conspicuous modification of the structure of the microfilament bundles is observed. Actin containing rod-like elements can be visualized both by phase contrast and immunofluorescence microscopy. These actin containing rods are of rather defined length (approximate length 5 μm) and seem to be derived as subunits from the original microfilament bundles. In some cells the rods were in the same orientation as the microfilament bundles in control cells, whereas other cells showed scattered arrangements. The phenomenon suggests intrafibrillar periodical heterogeneity in the microfilament bundles.
doi_str_mv 10.1016/0014-4827(77)90123-9
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subjects Actins
Cell Line
Cytochalasins - pharmacology
Cytoplasm - drug effects
Cytoskeleton - drug effects
Cytoskeleton - ultrastructure
Fluorescent Antibody Technique
title Rod-like elements from actin-containing microfilament bundles observed in cultured cells after treatment with cytochalasin A (CA)
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