Aequorin Luminescence: Relation of Light Emission to Calcium Concentration-A Calcium-Independent Component
Light emission from the calcium-sensitive bioluminescent protein aequorin was measured at calcium ion concentrations of 10$^{-9}$ to 10$^{-2}$ molar. At very low Ca$^{2+}$ concentrations, light emission is independent of calcium ion concentration. The maximum slope of the log-log plot of light as a...
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| Veröffentlicht in: | Science (American Association for the Advancement of Science) 1977-03, Vol.195 (4282), p.996-998 |
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| container_end_page | 998 |
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| container_issue | 4282 |
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| container_title | Science (American Association for the Advancement of Science) |
| container_volume | 195 |
| creator | Allen, David G. Blinks, John R. Prendergast, Franklyn G. |
| description | Light emission from the calcium-sensitive bioluminescent protein aequorin was measured at calcium ion concentrations of 10$^{-9}$ to 10$^{-2}$ molar. At very low Ca$^{2+}$ concentrations, light emission is independent of calcium ion concentration. The maximum slope of the log-log plot of light as a function of calcium ion concentration is about 2.5. The complete relation is well described by a two-state model involving three calcium-binding sites. |
| doi_str_mv | 10.1126/science.841325 |
| format | Article |
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At very low Ca$^{2+}$ concentrations, light emission is independent of calcium ion concentration. The maximum slope of the log-log plot of light as a function of calcium ion concentration is about 2.5. The complete relation is well described by a two-state model involving three calcium-binding sites.</description><identifier>ISSN: 0036-8075</identifier><identifier>EISSN: 1095-9203</identifier><identifier>DOI: 10.1126/science.841325</identifier><identifier>PMID: 841325</identifier><language>eng</language><publisher>United States: American Association for the Advancement of Science</publisher><subject>Aequorin - metabolism ; Binding Sites ; Calcium ; Calcium - analysis ; Calcium - metabolism ; Chromophores ; Kinetics ; Light emission ; Luminescence ; Luminous intensity ; Models, Biological ; Molecules ; Oxidation ; Oxygen ; Proteins - metabolism ; Reaction mechanisms</subject><ispartof>Science (American Association for the Advancement of Science), 1977-03, Vol.195 (4282), p.996-998</ispartof><rights>Copyright 1977 American Association for the Advancement of Science</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c406t-af2d4df989cc74286efadd8f1a4cb4e4465fbf43a347ad098a71759aac9ba2113</citedby><cites>FETCH-LOGICAL-c406t-af2d4df989cc74286efadd8f1a4cb4e4465fbf43a347ad098a71759aac9ba2113</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/1743733$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/1743733$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,2884,2885,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/841325$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Allen, David G.</creatorcontrib><creatorcontrib>Blinks, John R.</creatorcontrib><creatorcontrib>Prendergast, Franklyn G.</creatorcontrib><title>Aequorin Luminescence: Relation of Light Emission to Calcium Concentration-A Calcium-Independent Component</title><title>Science (American Association for the Advancement of Science)</title><addtitle>Science</addtitle><description>Light emission from the calcium-sensitive bioluminescent protein aequorin was measured at calcium ion concentrations of 10$^{-9}$ to 10$^{-2}$ molar. At very low Ca$^{2+}$ concentrations, light emission is independent of calcium ion concentration. The maximum slope of the log-log plot of light as a function of calcium ion concentration is about 2.5. The complete relation is well described by a two-state model involving three calcium-binding sites.</description><subject>Aequorin - metabolism</subject><subject>Binding Sites</subject><subject>Calcium</subject><subject>Calcium - analysis</subject><subject>Calcium - metabolism</subject><subject>Chromophores</subject><subject>Kinetics</subject><subject>Light emission</subject><subject>Luminescence</subject><subject>Luminous intensity</subject><subject>Models, Biological</subject><subject>Molecules</subject><subject>Oxidation</subject><subject>Oxygen</subject><subject>Proteins - metabolism</subject><subject>Reaction mechanisms</subject><issn>0036-8075</issn><issn>1095-9203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1977</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkL1PwzAQxS3EVymsTAyZ2FLs2ElstioqUCkSEoI5chwbXCV2azsD_z0uKSCx3J3e_e7p9AC4RnCBUFbceaGlEXJBCcJZfgRmCLI8ZRnEx2AGIS5SCsv8HFx4v4Ew7hg-A6cTPQObpdyN1mmT1OOgjfRib3afvMieB21NYlVS6_ePkKwG7f1eCTapeC_0OCSVjbAJ7htNlz96ujad3MpYTIjMsLUmTpfgRPHey6tDn4O3h9Vr9ZTWz4_ralmngsAipFxlHekUo0yIkmS0kIp3HVWIE9ESSUiRq1YRzDEpeQcZ5SUqc8a5YC3PEMJzcDv5bp3djdKHJn4uZN9zI-3oG4opITDGMAeLCRTOeu-karZOD9x9Ngg2-2ybQ7bNlFY8uDk4j-0gu1_8_3rjg3V_ZiXBJcb4C8ewgaU</recordid><startdate>19770311</startdate><enddate>19770311</enddate><creator>Allen, David G.</creator><creator>Blinks, John R.</creator><creator>Prendergast, Franklyn G.</creator><general>American Association for the Advancement of Science</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19770311</creationdate><title>Aequorin Luminescence: Relation of Light Emission to Calcium Concentration-A Calcium-Independent Component</title><author>Allen, David G. ; Blinks, John R. ; Prendergast, Franklyn G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c406t-af2d4df989cc74286efadd8f1a4cb4e4465fbf43a347ad098a71759aac9ba2113</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1977</creationdate><topic>Aequorin - metabolism</topic><topic>Binding Sites</topic><topic>Calcium</topic><topic>Calcium - analysis</topic><topic>Calcium - metabolism</topic><topic>Chromophores</topic><topic>Kinetics</topic><topic>Light emission</topic><topic>Luminescence</topic><topic>Luminous intensity</topic><topic>Models, Biological</topic><topic>Molecules</topic><topic>Oxidation</topic><topic>Oxygen</topic><topic>Proteins - metabolism</topic><topic>Reaction mechanisms</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Allen, David G.</creatorcontrib><creatorcontrib>Blinks, John R.</creatorcontrib><creatorcontrib>Prendergast, Franklyn G.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Science (American Association for the Advancement of Science)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Allen, David G.</au><au>Blinks, John R.</au><au>Prendergast, Franklyn G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Aequorin Luminescence: Relation of Light Emission to Calcium Concentration-A Calcium-Independent Component</atitle><jtitle>Science (American Association for the Advancement of Science)</jtitle><addtitle>Science</addtitle><date>1977-03-11</date><risdate>1977</risdate><volume>195</volume><issue>4282</issue><spage>996</spage><epage>998</epage><pages>996-998</pages><issn>0036-8075</issn><eissn>1095-9203</eissn><abstract>Light emission from the calcium-sensitive bioluminescent protein aequorin was measured at calcium ion concentrations of 10$^{-9}$ to 10$^{-2}$ molar. At very low Ca$^{2+}$ concentrations, light emission is independent of calcium ion concentration. The maximum slope of the log-log plot of light as a function of calcium ion concentration is about 2.5. The complete relation is well described by a two-state model involving three calcium-binding sites.</abstract><cop>United States</cop><pub>American Association for the Advancement of Science</pub><pmid>841325</pmid><doi>10.1126/science.841325</doi><tpages>3</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0036-8075 |
| ispartof | Science (American Association for the Advancement of Science), 1977-03, Vol.195 (4282), p.996-998 |
| issn | 0036-8075 1095-9203 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_83844059 |
| source | MEDLINE; JSTOR Archive Collection A-Z Listing; American Association for the Advancement of Science |
| subjects | Aequorin - metabolism Binding Sites Calcium Calcium - analysis Calcium - metabolism Chromophores Kinetics Light emission Luminescence Luminous intensity Models, Biological Molecules Oxidation Oxygen Proteins - metabolism Reaction mechanisms |
| title | Aequorin Luminescence: Relation of Light Emission to Calcium Concentration-A Calcium-Independent Component |
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