Synthesis of Methotrexate Polyglutamates in Cultured Human Cells
We describe the biosynthesis of po1y-γ-glutamyl metabolites of methotrexate in cultured human fibroblasts. The accumulation of polyglutamates by these cells is dependent on the concentration of methotrexate in the culture medium, duration of incubation, and stage of the culture cycle. When [3H]metho...
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| Veröffentlicht in: | Molecular pharmacology 1978-01, Vol.14 (1), p.210-214 |
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| container_title | Molecular pharmacology |
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| creator | ROSENBLATT, D.S. WHITEHEAD, V.M. DUPONT, M.M. VUCHICH, M.-J. VERA, N. |
| description | We describe the biosynthesis of po1y-γ-glutamyl metabolites of methotrexate in cultured human fibroblasts. The accumulation of polyglutamates by these cells is dependent on the concentration of methotrexate in the culture medium, duration of incubation, and stage of the culture cycle. When [3H]methotrexate is replaced with equimolar unlabeled methotrexate, label is found in successively longer polyglutamates. Synthesis of the polyglutamates is dependent on the continued presence of methotrexate in the culture medium. |
| doi_str_mv | 10.1016/S0026-895X(25)13337-3 |
| format | Article |
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The accumulation of polyglutamates by these cells is dependent on the concentration of methotrexate in the culture medium, duration of incubation, and stage of the culture cycle. When [3H]methotrexate is replaced with equimolar unlabeled methotrexate, label is found in successively longer polyglutamates. 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The accumulation of polyglutamates by these cells is dependent on the concentration of methotrexate in the culture medium, duration of incubation, and stage of the culture cycle. When [3H]methotrexate is replaced with equimolar unlabeled methotrexate, label is found in successively longer polyglutamates. Synthesis of the polyglutamates is dependent on the continued presence of methotrexate in the culture medium.</description><subject>Cells, Cultured</subject><subject>Fibroblasts - metabolism</subject><subject>Glutamates - metabolism</subject><subject>Humans</subject><subject>Kinetics</subject><subject>Methotrexate - metabolism</subject><subject>Time Factors</subject><issn>0026-895X</issn><issn>1521-0111</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1978</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMFu1DAQhi1EKdvSNyhSLlT0EPDYceKcaLWCFqkIpAWpN8txJhujZL21nZZ9e7xN1WvlgzWa758ZfYS8B_oJKJSfV5SyMpe1uP3IxDlwzqucvyILEAxyCgCvyeIZeUuOQvhLKRRC0kPypmSCyXJBLla7Tewx2JC5LvuBsXfR4z8dMfvlht16mKIeUxUyu8mW0xAnj212PY06lTgM4R056PQQ8OTpPyZ_vn39vbzOb35efV9e3uSGlXXMO8HrkjZQcA111UhamUqaRhRtoXlVG8lRItYNBUHrikrGW0F52aEpKsC24cfkbJ679e5uwhDVaINJF-gNuikoydNjdZlAMYPGuxA8dmrr7aj9TgFVe3HqUZzaW1FMqEdxiqfc6dOCqRmxfU7NplL7w9zu7bp_sB7Vttd-1MYNbp1mFwoUA5q4LzOHSca9Ra-Csbgx2KaMiap19oVD_gOCU4n3</recordid><startdate>197801</startdate><enddate>197801</enddate><creator>ROSENBLATT, D.S.</creator><creator>WHITEHEAD, V.M.</creator><creator>DUPONT, M.M.</creator><creator>VUCHICH, M.-J.</creator><creator>VERA, N.</creator><general>Elsevier Inc</general><general>American Society for Pharmacology and Experimental Therapeutics</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197801</creationdate><title>Synthesis of Methotrexate Polyglutamates in Cultured Human Cells</title><author>ROSENBLATT, D.S. ; WHITEHEAD, V.M. ; DUPONT, M.M. ; VUCHICH, M.-J. ; VERA, N.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c269t-f53960b143a197b807c78cb54d4a379c83e8ee9b0150970823d5036fec471edb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1978</creationdate><topic>Cells, Cultured</topic><topic>Fibroblasts - metabolism</topic><topic>Glutamates - metabolism</topic><topic>Humans</topic><topic>Kinetics</topic><topic>Methotrexate - metabolism</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ROSENBLATT, D.S.</creatorcontrib><creatorcontrib>WHITEHEAD, V.M.</creatorcontrib><creatorcontrib>DUPONT, M.M.</creatorcontrib><creatorcontrib>VUCHICH, M.-J.</creatorcontrib><creatorcontrib>VERA, N.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ROSENBLATT, D.S.</au><au>WHITEHEAD, V.M.</au><au>DUPONT, M.M.</au><au>VUCHICH, M.-J.</au><au>VERA, N.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Synthesis of Methotrexate Polyglutamates in Cultured Human Cells</atitle><jtitle>Molecular pharmacology</jtitle><addtitle>Mol Pharmacol</addtitle><date>1978-01</date><risdate>1978</risdate><volume>14</volume><issue>1</issue><spage>210</spage><epage>214</epage><pages>210-214</pages><issn>0026-895X</issn><eissn>1521-0111</eissn><abstract>We describe the biosynthesis of po1y-γ-glutamyl metabolites of methotrexate in cultured human fibroblasts. The accumulation of polyglutamates by these cells is dependent on the concentration of methotrexate in the culture medium, duration of incubation, and stage of the culture cycle. When [3H]methotrexate is replaced with equimolar unlabeled methotrexate, label is found in successively longer polyglutamates. Synthesis of the polyglutamates is dependent on the continued presence of methotrexate in the culture medium.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>625286</pmid><doi>10.1016/S0026-895X(25)13337-3</doi><tpages>5</tpages></addata></record> |
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| ispartof | Molecular pharmacology, 1978-01, Vol.14 (1), p.210-214 |
| issn | 0026-895X 1521-0111 |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Cells, Cultured Fibroblasts - metabolism Glutamates - metabolism Humans Kinetics Methotrexate - metabolism Time Factors |
| title | Synthesis of Methotrexate Polyglutamates in Cultured Human Cells |
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