Subcellular localization of cyclic nucleotide phosphodiesterase in the caudate nucleus
The subcellular localization of cyclic nucleotide phosphodiesterase activity is described in the caudate nucleus and the neocortex of the cat and rat. Reaction product is visualized postsynaptically at asymmetrical axodendritic synapses by the statu nascendi precipitation of lead phosphate. The effe...
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| Veröffentlicht in: | Experimental neurology 1977-04, Vol.55 (1), p.84-94 |
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| container_title | Experimental neurology |
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| creator | Ariano, M.A. Adinolfi, A.M. |
| description | The subcellular localization of cyclic nucleotide phosphodiesterase activity is described in the caudate nucleus and the neocortex of the cat and rat. Reaction product is visualized postsynaptically at asymmetrical axodendritic synapses by the
statu nascendi precipitation of lead phosphate. The effect of varying substrate concentrations and incubation times to localize enzyme activity at cortical and caudate synapses in the two species is discussed. Demonstration of phosphodiesterase activity at postsynaptic sites adds further indirect evidence suggesting a role for cyclic nucleotides in synaptic transmission. |
| doi_str_mv | 10.1016/0014-4886(77)90160-1 |
| format | Article |
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statu nascendi precipitation of lead phosphate. The effect of varying substrate concentrations and incubation times to localize enzyme activity at cortical and caudate synapses in the two species is discussed. Demonstration of phosphodiesterase activity at postsynaptic sites adds further indirect evidence suggesting a role for cyclic nucleotides in synaptic transmission.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>191273</pmid><doi>10.1016/0014-4886(77)90160-1</doi><tpages>11</tpages></addata></record> |
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| ispartof | Experimental neurology, 1977-04, Vol.55 (1), p.84-94 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | 2',3'-Cyclic-Nucleotide Phosphodiesterases - analysis Animals Cats Caudate Nucleus - enzymology Cerebral Cortex - enzymology Phosphoric Diester Hydrolases - analysis Rats Synaptic Membranes - enzymology |
| title | Subcellular localization of cyclic nucleotide phosphodiesterase in the caudate nucleus |
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