Specific alterations in phosphorylation of cytosol proteins from differentiating neuroblastoma cells grown in culture

THERE have been a number of reports 1–10 which indicate that an experimentally induced elevation in the intracellular level of adenosine-3′,5′-monophosphate (cyclic AMP) in mouse neuroblastoma cells induces and increases the expression of many differentiated functions that are characteristic of the...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Nature (London) 1977-01, Vol.265 (5591), p.238-240
Hauptverfasser: EHRLICH, YIGAL H, BRUNNGRABER, ERIC G, SINHA, PRAMOD K, PRASAD, KEDAR N
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 240
container_issue 5591
container_start_page 238
container_title Nature (London)
container_volume 265
creator EHRLICH, YIGAL H
BRUNNGRABER, ERIC G
SINHA, PRAMOD K
PRASAD, KEDAR N
description THERE have been a number of reports 1–10 which indicate that an experimentally induced elevation in the intracellular level of adenosine-3′,5′-monophosphate (cyclic AMP) in mouse neuroblastoma cells induces and increases the expression of many differentiated functions that are characteristic of the mature neurone. The irreversibility of cyclic AMP-induced “differentiation” 3 was attributed to the increased level of proteins which bind cyclic AMP thereby protecting the newly synthesised cyclic nucleotide from hydrolysis 11,12 . However, the mechanisms by which cyclic AMP initiates the observed morphological 1–3 and biochemical 3–10 alterations remain unclear since the activity of cyclic AMP-dependent protein kinase did not change in differentiated cells 13 . We have investigated the phosphorylation of specific proteins in control and ‘differentiated’ neuroblastoma cells. We report here that the cyclic AMP-dependent phosphorylation of a specific protein in the cytosol of ‘differentiated’ cells increased while the cyclic AMP-independent phosphorylation of another protein in this cell fraction decreased. These selective alterations may represent intermediate steps in a series of events which ultimately lead to the expression of differentiated functions.
doi_str_mv 10.1038/265238a0
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_83836479</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>83836479</sourcerecordid><originalsourceid>FETCH-LOGICAL-c334t-4d7e3232a784c4571947554edee1fde6d5a57026bb85933008899068cc9458083</originalsourceid><addsrcrecordid>eNptkMtKxDAUhoN4Gy_gA4hkJbqopk3SpEsRbyC4UNclk56OGdpkTFJk3t50RseNi3Ag_8fPOR9CJzm5ygmV10XJCyoV2UKTnIkyY6UU22hCSCEzImm5jw5CmBNCeC7YHtrNZVUQMUHD6wK0aY3GqovgVTTOBmwsXny4kJ5fdqs_7Fqsl9EF1-GFdxFMwlrvetyYtgUPNpoE2hm2MHg37VSIrldYQ9cFPPPuy46teuji4OEI7bSqC3D8Mw_R-_3d2-1j9vzy8HR785xpSlnMWCOAFrRQQjLNuMgrJjhn0ADkbQNlwxUXpCinU8krSgmRsqpIKbWuGJfp7EN0vu5NK38OEGLdmzCupCy4IdSSJjVMVAm8WIPauxA8tPXCm175ZZ2TehRc_wpO6OlP5zDtofkDV0ZTfLmOQwrsDHw9d4O36cr_qs7WrFWjlU3VBvgGVI-OsQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>83836479</pqid></control><display><type>article</type><title>Specific alterations in phosphorylation of cytosol proteins from differentiating neuroblastoma cells grown in culture</title><source>MEDLINE</source><source>Nature</source><source>Alma/SFX Local Collection</source><creator>EHRLICH, YIGAL H ; BRUNNGRABER, ERIC G ; SINHA, PRAMOD K ; PRASAD, KEDAR N</creator><creatorcontrib>EHRLICH, YIGAL H ; BRUNNGRABER, ERIC G ; SINHA, PRAMOD K ; PRASAD, KEDAR N</creatorcontrib><description>THERE have been a number of reports 1–10 which indicate that an experimentally induced elevation in the intracellular level of adenosine-3′,5′-monophosphate (cyclic AMP) in mouse neuroblastoma cells induces and increases the expression of many differentiated functions that are characteristic of the mature neurone. The irreversibility of cyclic AMP-induced “differentiation” 3 was attributed to the increased level of proteins which bind cyclic AMP thereby protecting the newly synthesised cyclic nucleotide from hydrolysis 11,12 . However, the mechanisms by which cyclic AMP initiates the observed morphological 1–3 and biochemical 3–10 alterations remain unclear since the activity of cyclic AMP-dependent protein kinase did not change in differentiated cells 13 . We have investigated the phosphorylation of specific proteins in control and ‘differentiated’ neuroblastoma cells. We report here that the cyclic AMP-dependent phosphorylation of a specific protein in the cytosol of ‘differentiated’ cells increased while the cyclic AMP-independent phosphorylation of another protein in this cell fraction decreased. These selective alterations may represent intermediate steps in a series of events which ultimately lead to the expression of differentiated functions.</description><identifier>ISSN: 0028-0836</identifier><identifier>EISSN: 1476-4687</identifier><identifier>DOI: 10.1038/265238a0</identifier><identifier>PMID: 189207</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>4-(3-Butoxy-4-methoxybenzyl)-2-imidazolidinone - pharmacology ; Cell Differentiation - drug effects ; Cells, Cultured ; Cyclic AMP - metabolism ; Cytosol - metabolism ; Humanities and Social Sciences ; letter ; multidisciplinary ; Nerve Tissue Proteins - metabolism ; Neuroblastoma ; Neurons - cytology ; Neurons - metabolism ; Phosphates - metabolism ; Science ; Science (multidisciplinary)</subject><ispartof>Nature (London), 1977-01, Vol.265 (5591), p.238-240</ispartof><rights>Springer Nature Limited 1977</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c334t-4d7e3232a784c4571947554edee1fde6d5a57026bb85933008899068cc9458083</citedby><cites>FETCH-LOGICAL-c334t-4d7e3232a784c4571947554edee1fde6d5a57026bb85933008899068cc9458083</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,2727,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/189207$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>EHRLICH, YIGAL H</creatorcontrib><creatorcontrib>BRUNNGRABER, ERIC G</creatorcontrib><creatorcontrib>SINHA, PRAMOD K</creatorcontrib><creatorcontrib>PRASAD, KEDAR N</creatorcontrib><title>Specific alterations in phosphorylation of cytosol proteins from differentiating neuroblastoma cells grown in culture</title><title>Nature (London)</title><addtitle>Nature</addtitle><addtitle>Nature</addtitle><description>THERE have been a number of reports 1–10 which indicate that an experimentally induced elevation in the intracellular level of adenosine-3′,5′-monophosphate (cyclic AMP) in mouse neuroblastoma cells induces and increases the expression of many differentiated functions that are characteristic of the mature neurone. The irreversibility of cyclic AMP-induced “differentiation” 3 was attributed to the increased level of proteins which bind cyclic AMP thereby protecting the newly synthesised cyclic nucleotide from hydrolysis 11,12 . However, the mechanisms by which cyclic AMP initiates the observed morphological 1–3 and biochemical 3–10 alterations remain unclear since the activity of cyclic AMP-dependent protein kinase did not change in differentiated cells 13 . We have investigated the phosphorylation of specific proteins in control and ‘differentiated’ neuroblastoma cells. We report here that the cyclic AMP-dependent phosphorylation of a specific protein in the cytosol of ‘differentiated’ cells increased while the cyclic AMP-independent phosphorylation of another protein in this cell fraction decreased. These selective alterations may represent intermediate steps in a series of events which ultimately lead to the expression of differentiated functions.</description><subject>4-(3-Butoxy-4-methoxybenzyl)-2-imidazolidinone - pharmacology</subject><subject>Cell Differentiation - drug effects</subject><subject>Cells, Cultured</subject><subject>Cyclic AMP - metabolism</subject><subject>Cytosol - metabolism</subject><subject>Humanities and Social Sciences</subject><subject>letter</subject><subject>multidisciplinary</subject><subject>Nerve Tissue Proteins - metabolism</subject><subject>Neuroblastoma</subject><subject>Neurons - cytology</subject><subject>Neurons - metabolism</subject><subject>Phosphates - metabolism</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><issn>0028-0836</issn><issn>1476-4687</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1977</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkMtKxDAUhoN4Gy_gA4hkJbqopk3SpEsRbyC4UNclk56OGdpkTFJk3t50RseNi3Ag_8fPOR9CJzm5ygmV10XJCyoV2UKTnIkyY6UU22hCSCEzImm5jw5CmBNCeC7YHtrNZVUQMUHD6wK0aY3GqovgVTTOBmwsXny4kJ5fdqs_7Fqsl9EF1-GFdxFMwlrvetyYtgUPNpoE2hm2MHg37VSIrldYQ9cFPPPuy46teuji4OEI7bSqC3D8Mw_R-_3d2-1j9vzy8HR785xpSlnMWCOAFrRQQjLNuMgrJjhn0ADkbQNlwxUXpCinU8krSgmRsqpIKbWuGJfp7EN0vu5NK38OEGLdmzCupCy4IdSSJjVMVAm8WIPauxA8tPXCm175ZZ2TehRc_wpO6OlP5zDtofkDV0ZTfLmOQwrsDHw9d4O36cr_qs7WrFWjlU3VBvgGVI-OsQ</recordid><startdate>19770120</startdate><enddate>19770120</enddate><creator>EHRLICH, YIGAL H</creator><creator>BRUNNGRABER, ERIC G</creator><creator>SINHA, PRAMOD K</creator><creator>PRASAD, KEDAR N</creator><general>Nature Publishing Group UK</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19770120</creationdate><title>Specific alterations in phosphorylation of cytosol proteins from differentiating neuroblastoma cells grown in culture</title><author>EHRLICH, YIGAL H ; BRUNNGRABER, ERIC G ; SINHA, PRAMOD K ; PRASAD, KEDAR N</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c334t-4d7e3232a784c4571947554edee1fde6d5a57026bb85933008899068cc9458083</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1977</creationdate><topic>4-(3-Butoxy-4-methoxybenzyl)-2-imidazolidinone - pharmacology</topic><topic>Cell Differentiation - drug effects</topic><topic>Cells, Cultured</topic><topic>Cyclic AMP - metabolism</topic><topic>Cytosol - metabolism</topic><topic>Humanities and Social Sciences</topic><topic>letter</topic><topic>multidisciplinary</topic><topic>Nerve Tissue Proteins - metabolism</topic><topic>Neuroblastoma</topic><topic>Neurons - cytology</topic><topic>Neurons - metabolism</topic><topic>Phosphates - metabolism</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>EHRLICH, YIGAL H</creatorcontrib><creatorcontrib>BRUNNGRABER, ERIC G</creatorcontrib><creatorcontrib>SINHA, PRAMOD K</creatorcontrib><creatorcontrib>PRASAD, KEDAR N</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Nature (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>EHRLICH, YIGAL H</au><au>BRUNNGRABER, ERIC G</au><au>SINHA, PRAMOD K</au><au>PRASAD, KEDAR N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Specific alterations in phosphorylation of cytosol proteins from differentiating neuroblastoma cells grown in culture</atitle><jtitle>Nature (London)</jtitle><stitle>Nature</stitle><addtitle>Nature</addtitle><date>1977-01-20</date><risdate>1977</risdate><volume>265</volume><issue>5591</issue><spage>238</spage><epage>240</epage><pages>238-240</pages><issn>0028-0836</issn><eissn>1476-4687</eissn><abstract>THERE have been a number of reports 1–10 which indicate that an experimentally induced elevation in the intracellular level of adenosine-3′,5′-monophosphate (cyclic AMP) in mouse neuroblastoma cells induces and increases the expression of many differentiated functions that are characteristic of the mature neurone. The irreversibility of cyclic AMP-induced “differentiation” 3 was attributed to the increased level of proteins which bind cyclic AMP thereby protecting the newly synthesised cyclic nucleotide from hydrolysis 11,12 . However, the mechanisms by which cyclic AMP initiates the observed morphological 1–3 and biochemical 3–10 alterations remain unclear since the activity of cyclic AMP-dependent protein kinase did not change in differentiated cells 13 . We have investigated the phosphorylation of specific proteins in control and ‘differentiated’ neuroblastoma cells. We report here that the cyclic AMP-dependent phosphorylation of a specific protein in the cytosol of ‘differentiated’ cells increased while the cyclic AMP-independent phosphorylation of another protein in this cell fraction decreased. These selective alterations may represent intermediate steps in a series of events which ultimately lead to the expression of differentiated functions.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>189207</pmid><doi>10.1038/265238a0</doi><tpages>3</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0028-0836
ispartof Nature (London), 1977-01, Vol.265 (5591), p.238-240
issn 0028-0836
1476-4687
language eng
recordid cdi_proquest_miscellaneous_83836479
source MEDLINE; Nature; Alma/SFX Local Collection
subjects 4-(3-Butoxy-4-methoxybenzyl)-2-imidazolidinone - pharmacology
Cell Differentiation - drug effects
Cells, Cultured
Cyclic AMP - metabolism
Cytosol - metabolism
Humanities and Social Sciences
letter
multidisciplinary
Nerve Tissue Proteins - metabolism
Neuroblastoma
Neurons - cytology
Neurons - metabolism
Phosphates - metabolism
Science
Science (multidisciplinary)
title Specific alterations in phosphorylation of cytosol proteins from differentiating neuroblastoma cells grown in culture
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T21%3A44%3A04IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Specific%20alterations%20in%20phosphorylation%20of%20cytosol%20proteins%20from%20differentiating%20neuroblastoma%20cells%20grown%20in%20culture&rft.jtitle=Nature%20(London)&rft.au=EHRLICH,%20YIGAL%20H&rft.date=1977-01-20&rft.volume=265&rft.issue=5591&rft.spage=238&rft.epage=240&rft.pages=238-240&rft.issn=0028-0836&rft.eissn=1476-4687&rft_id=info:doi/10.1038/265238a0&rft_dat=%3Cproquest_cross%3E83836479%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=83836479&rft_id=info:pmid/189207&rfr_iscdi=true