Nucleotide specificity in microtubule assembly in vitro

A procedure is described for removing most of the GDP bound at the exchangeable GTP binding site (E site) of tubulin. Microtubule protein containing substoichiometric amounts of GDP at the E site is found to polymerize in response to: (a) two nonhydrolyzable ATP analogues, adenylyl imidodiphosphate...

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Veröffentlicht in:	Biochemistry (Easton) 1978-02, Vol.17 (4), p.734-740
Hauptverfasser:	Penningroth, Stephen M, Kirschner, Marc W
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Triphosphate - analogs & derivatives Animals Brain Glycoproteins Guanosine Diphosphate Guanosine Triphosphate Kinetics Microtubules - ultrastructure Nerve Tissue Proteins Protein Binding Swine Tubulin
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container_title	Biochemistry (Easton)
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creator	Penningroth, Stephen M Kirschner, Marc W
description	A procedure is described for removing most of the GDP bound at the exchangeable GTP binding site (E site) of tubulin. Microtubule protein containing substoichiometric amounts of GDP at the E site is found to polymerize in response to: (a) two nonhydrolyzable ATP analogues, adenylyl imidodiphosphate (AMP-PNP) and adenylyl beta, gamma-methylenediphosphonate (AMP-PCP); and (b) substoichiometric levels of GTP or dGTP. The results are interpreted as suggesting that: (1) when GDP is removed from tubulin, the E site shows broad specificity for nucleoside triphosphates: (2) microtubule assembly can be induced by the binding of substoichiometric amounts of nucleoside triphosphate to the E site.
doi_str_mv	10.1021/bi00597a028
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Microtubule protein containing substoichiometric amounts of GDP at the E site is found to polymerize in response to: (a) two nonhydrolyzable ATP analogues, adenylyl imidodiphosphate (AMP-PNP) and adenylyl beta, gamma-methylenediphosphonate (AMP-PCP); and (b) substoichiometric levels of GTP or dGTP. 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subjects	Adenosine Triphosphate - analogs & derivatives Animals Brain Glycoproteins Guanosine Diphosphate Guanosine Triphosphate Kinetics Microtubules - ultrastructure Nerve Tissue Proteins Protein Binding Swine Tubulin
title	Nucleotide specificity in microtubule assembly in vitro
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