Kinetic Studies on a 4‐Methoxybenzoate O‐Demethylase from Pseudomonas putida

A direct, sensitive and reliable photometric assay procedure for monitoring the activity of nonspecific 4‐methoxybenzoate O‐demethylases of microorganisms is described. The assay is based on the O‐demethylation of 3‐nitro‐4‐methoxybenzoate to the yellow‐coloured product 3‐nitro‐4‐hydroxybenzoate. Us...

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Veröffentlicht in:	European journal of biochemistry 1977-01, Vol.72 (1), p.107-115
Hauptverfasser:	BERNHARDT, Frithjof‐Hans, NASTAINCZYK, Wolfgang, SEYDEWITZ, Vera
Format:	Artikel
Sprache:	eng
Schlagworte:	Benzoates - pharmacology Kinetics NAD Oxidoreductases - metabolism Oxidoreductases, O-Demethylating - metabolism Pseudomonas - enzymology Structure-Activity Relationship
Online-Zugang:	Volltext
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