Radioiodinated Plasminogen: An Imaging Agent for Pre-Existing Thrombi
We have reinvestigated radioiodinated plasminogen as an agent for localizing preformed thrombi. Canine plasminogen was isolated from fresh plasma by the affinity chromatography technique on a lysine-sepharose 4B column and tagged with I-123 or I-131, at less than one iodine atom per molecule of enzy...
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| Veröffentlicht in: | The Journal of nuclear medicine (1978) 1977-01, Vol.18 (1), p.42-45 |
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| container_title | The Journal of nuclear medicine (1978) |
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| creator | Harwig, Sylvia S. L Harwig, John F Sherman, Laurence A Coleman, R. Edward Welch, Michael J |
| description | We have reinvestigated radioiodinated plasminogen as an agent for localizing preformed thrombi. Canine plasminogen was isolated from fresh plasma by the affinity chromatography technique on a lysine-sepharose 4B column and tagged with I-123 or I-131, at less than one iodine atom per molecule of enzyme, by the conventional ICI method. When injected into dogs more than 2 days after thrombus induction, radioiodinated plasminogen produced thrombus-to-blood activity ratios of 7.8 +/- 2.4. Thrombi as old as 6 days can be visualized in 80% of the cases. Both the weight of the thrombus and the thrombus-to-blood ratio are more variable for 1-day-old thrombi; this may be associated with plasminogen release accompanying thrombus retraction. The results suggest that radioiodinated plasminogen has potential as an imaging agent for pre-existing thrombi. |
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L ; Harwig, John F ; Sherman, Laurence A ; Coleman, R. Edward ; Welch, Michael J</creator><creatorcontrib>Harwig, Sylvia S. L ; Harwig, John F ; Sherman, Laurence A ; Coleman, R. Edward ; Welch, Michael J</creatorcontrib><description>We have reinvestigated radioiodinated plasminogen as an agent for localizing preformed thrombi. Canine plasminogen was isolated from fresh plasma by the affinity chromatography technique on a lysine-sepharose 4B column and tagged with I-123 or I-131, at less than one iodine atom per molecule of enzyme, by the conventional ICI method. When injected into dogs more than 2 days after thrombus induction, radioiodinated plasminogen produced thrombus-to-blood activity ratios of 7.8 +/- 2.4. Thrombi as old as 6 days can be visualized in 80% of the cases. Both the weight of the thrombus and the thrombus-to-blood ratio are more variable for 1-day-old thrombi; this may be associated with plasminogen release accompanying thrombus retraction. The results suggest that radioiodinated plasminogen has potential as an imaging agent for pre-existing thrombi.</description><identifier>ISSN: 0161-5505</identifier><identifier>EISSN: 1535-5667</identifier><identifier>PMID: 830827</identifier><language>eng</language><publisher>United States: Soc Nuclear Med</publisher><subject>Animals ; Dogs ; Iodine Radioisotopes ; Plasminogen ; Radionuclide Imaging ; Thrombophlebitis - diagnosis</subject><ispartof>The Journal of nuclear medicine (1978), 1977-01, Vol.18 (1), p.42-45</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/830827$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Harwig, Sylvia S. 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Both the weight of the thrombus and the thrombus-to-blood ratio are more variable for 1-day-old thrombi; this may be associated with plasminogen release accompanying thrombus retraction. The results suggest that radioiodinated plasminogen has potential as an imaging agent for pre-existing thrombi.</description><subject>Animals</subject><subject>Dogs</subject><subject>Iodine Radioisotopes</subject><subject>Plasminogen</subject><subject>Radionuclide Imaging</subject><subject>Thrombophlebitis - diagnosis</subject><issn>0161-5505</issn><issn>1535-5667</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1977</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNotj11LwzAYhYP4Naf_wIve6F0hH02TejfG1MHAIfM6pM3bNqNJZ9Ki_ns7tqsD5zwceC7QjHDGU57n4hLNMMlJyjnmt-guxj3GOJdS3qBrybCkYoZWn9rY3vbGej2ASbadjs76vgH_kix8sna6sb5JFlMxJHUfkm2AdPVr43Csd23oXWnv0VWtuwgP55yjr9fVbvmebj7e1svFJm0p40NKsTZCZHVVsExkVOe8hKrklZF1zTiIwmBDaC1yCdywSaHKsSmx0DQroIKCzdHz6fcQ-u8R4qCcjRV0nfbQj1FJJjFhIpvAxzM4lg6MOgTrdPhTJ-1pfjrNrW3aHxtA-bHqQIcju_eOSEVURtk_YkZheQ</recordid><startdate>19770101</startdate><enddate>19770101</enddate><creator>Harwig, Sylvia S. 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Edward ; Welch, Michael J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h235t-20ad774fc934742a65becb5cd8ff35e79d0d12f768e5d3566c60db07a249ece93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1977</creationdate><topic>Animals</topic><topic>Dogs</topic><topic>Iodine Radioisotopes</topic><topic>Plasminogen</topic><topic>Radionuclide Imaging</topic><topic>Thrombophlebitis - diagnosis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Harwig, Sylvia S. L</creatorcontrib><creatorcontrib>Harwig, John F</creatorcontrib><creatorcontrib>Sherman, Laurence A</creatorcontrib><creatorcontrib>Coleman, R. Edward</creatorcontrib><creatorcontrib>Welch, Michael J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of nuclear medicine (1978)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Harwig, Sylvia S. L</au><au>Harwig, John F</au><au>Sherman, Laurence A</au><au>Coleman, R. 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Thrombi as old as 6 days can be visualized in 80% of the cases. Both the weight of the thrombus and the thrombus-to-blood ratio are more variable for 1-day-old thrombi; this may be associated with plasminogen release accompanying thrombus retraction. The results suggest that radioiodinated plasminogen has potential as an imaging agent for pre-existing thrombi.</abstract><cop>United States</cop><pub>Soc Nuclear Med</pub><pmid>830827</pmid><tpages>4</tpages></addata></record> |
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| ispartof | The Journal of nuclear medicine (1978), 1977-01, Vol.18 (1), p.42-45 |
| issn | 0161-5505 1535-5667 |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Animals Dogs Iodine Radioisotopes Plasminogen Radionuclide Imaging Thrombophlebitis - diagnosis |
| title | Radioiodinated Plasminogen: An Imaging Agent for Pre-Existing Thrombi |
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