Quantitative aspects of the binding of nuclear non-histone proteins to DNA as determined by centrifugation in metrizamide gradients
Density-gradient centrifugation in metrizamide gradients has been used to study the binding of nuclear proteins to DNA. The unique advantage of this method is that the nucleoprotein complexes can be isolated free of non-complexed DNA and proteins. The chromatin non-histone proteins bound to native D...
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| Veröffentlicht in: | Experimental cell research 1977-02, Vol.104 (2), p.287-292 |
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| container_title | Experimental cell research |
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| creator | Rickwood, D. MacGillivray, A.J. |
| description | Density-gradient centrifugation in metrizamide gradients has been used to study the binding of nuclear proteins to DNA. The unique advantage of this method is that the nucleoprotein complexes can be isolated free of non-complexed DNA and proteins. The chromatin non-histone proteins bound to native DNA in a non-random manner. The extent of binding was dependent on the ionic strength of the medium and was decreased in the presence of RNA. |
| doi_str_mv | 10.1016/0014-4827(77)90093-3 |
| format | Article |
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The extent of binding was dependent on the ionic strength of the medium and was decreased in the presence of RNA.</description><subject>Cell Line</subject><subject>Centrifugation, Density Gradient</subject><subject>Chromosomal Proteins, Non-Histone - metabolism</subject><subject>DNA - metabolism</subject><subject>DNA, Bacterial - metabolism</subject><subject>Escherichia coli</subject><subject>Metrizamide</subject><subject>Osmolar Concentration</subject><subject>RNA - pharmacology</subject><issn>0014-4827</issn><issn>1090-2422</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1977</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtOGzEUhq2q0AboG2ThVQWLAV9mxp5NJRSuEqKq1K4tj30muMrYqe2JBFteHIegLLs68vkv8vkQmlNyTgltLwihdVVLJk6FOOsI6XjFP6EZJR2pWM3YZzTbW76io5T-EkKkpO0XdMhZkcQMvf6atM8u6-w2gHVag8kJhwHnJ8C989b55fbpJ7MCHbEPvnpyKQcPeB1DBucTzgFfPV6WNLaQIY7Og8X9Mzbgc3TDtCztwWPn8Qhl8aJHZwEvo7auONIJOhj0KsG3j3mM_txc_17cVQ8_b-8Xlw-V4U2bK5Bcd9AJUddysE1DJW2oZVzyoRM1BTYMIAnlLRG8bzoK2oiiy0Yy1vct48fo-663fPzfBCmr0SUDq5X2EKakJBeiFQ0txnpnNDGkFGFQ6-hGHZ8VJWqLXm25qi1XJYR6R694ic0_-qd-BLsP7VgX-cdOhnLjxkFUyZT7DVgXC3Vlg_t__xt4t5P4</recordid><startdate>197702</startdate><enddate>197702</enddate><creator>Rickwood, D.</creator><creator>MacGillivray, A.J.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197702</creationdate><title>Quantitative aspects of the binding of nuclear non-histone proteins to DNA as determined by centrifugation in metrizamide gradients</title><author>Rickwood, D. ; MacGillivray, A.J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-e83a9e977448fd5518151d2383f9741e2ffe80136073b591eac71d285822bb623</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1977</creationdate><topic>Cell Line</topic><topic>Centrifugation, Density Gradient</topic><topic>Chromosomal Proteins, Non-Histone - metabolism</topic><topic>DNA - metabolism</topic><topic>DNA, Bacterial - metabolism</topic><topic>Escherichia coli</topic><topic>Metrizamide</topic><topic>Osmolar Concentration</topic><topic>RNA - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rickwood, D.</creatorcontrib><creatorcontrib>MacGillivray, A.J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental cell research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rickwood, D.</au><au>MacGillivray, A.J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantitative aspects of the binding of nuclear non-histone proteins to DNA as determined by centrifugation in metrizamide gradients</atitle><jtitle>Experimental cell research</jtitle><addtitle>Exp Cell Res</addtitle><date>1977-02</date><risdate>1977</risdate><volume>104</volume><issue>2</issue><spage>287</spage><epage>292</epage><pages>287-292</pages><issn>0014-4827</issn><eissn>1090-2422</eissn><abstract>Density-gradient centrifugation in metrizamide gradients has been used to study the binding of nuclear proteins to DNA. 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| ispartof | Experimental cell research, 1977-02, Vol.104 (2), p.287-292 |
| issn | 0014-4827 1090-2422 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Cell Line Centrifugation, Density Gradient Chromosomal Proteins, Non-Histone - metabolism DNA - metabolism DNA, Bacterial - metabolism Escherichia coli Metrizamide Osmolar Concentration RNA - pharmacology |
| title | Quantitative aspects of the binding of nuclear non-histone proteins to DNA as determined by centrifugation in metrizamide gradients |
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