Hepatitis B antigen in black patients with hepatocellular carcinoma. Correlation between orcein stained liver sections and serology
Formalin‐fixed paraffin‐embedded autopsy tissue of liver and tumor from 50 male black mineworkers with hepatocellular carcinoma were examined by orcein stain for the presence of cytoplasmic hepatitis B surface antigen. The results were correlated with the serum hepatitis B antigen (HBAg). In 72% ser...
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| Veröffentlicht in: | Cancer 1978-01, Vol.41 (1), p.245-249 |
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| creator | Cohen, Cynthia Berson, S. D. Geddes, E. W. |
| description | Formalin‐fixed paraffin‐embedded autopsy tissue of liver and tumor from 50 male black mineworkers with hepatocellular carcinoma were examined by orcein stain for the presence of cytoplasmic hepatitis B surface antigen. The results were correlated with the serum hepatitis B antigen (HBAg). In 72% serum HBAg was positive. Orcein staining of nontumor liver cell cytoplasm was present in 18 (36%). Sixteen (89%) of these orcein‐positive cases were serum HBAg positive. The two false negative serum HBAg results were obtained by immunodiffusion, immunoelectrophoresis and complement fixation. Serum HBAg, measured by radio‐immunoassay and hemagglutination, was positive in 14 orcein‐negative cases. Six other negative orcein results appeared to be due to sampling error. Orcein staining was noted in tumor cells of three serum HBAg positive patients. Provided the limitations of the technique are realized, orcein staining of liver tissue from hepatocellular carcinoma patients may prove useful for retrospective screening surveys to assess the prevalence of HBAg positivity in these patients. |
| doi_str_mv | 10.1002/1097-0142(197801)41:1<245::AID-CNCR2820410134>3.0.CO;2-4 |
| format | Article |
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Correlation between orcein stained liver sections and serology</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>Cohen, Cynthia ; Berson, S. D. ; Geddes, E. W.</creator><creatorcontrib>Cohen, Cynthia ; Berson, S. D. ; Geddes, E. W.</creatorcontrib><description>Formalin‐fixed paraffin‐embedded autopsy tissue of liver and tumor from 50 male black mineworkers with hepatocellular carcinoma were examined by orcein stain for the presence of cytoplasmic hepatitis B surface antigen. The results were correlated with the serum hepatitis B antigen (HBAg). In 72% serum HBAg was positive. Orcein staining of nontumor liver cell cytoplasm was present in 18 (36%). Sixteen (89%) of these orcein‐positive cases were serum HBAg positive. The two false negative serum HBAg results were obtained by immunodiffusion, immunoelectrophoresis and complement fixation. Serum HBAg, measured by radio‐immunoassay and hemagglutination, was positive in 14 orcein‐negative cases. Six other negative orcein results appeared to be due to sampling error. Orcein staining was noted in tumor cells of three serum HBAg positive patients. Provided the limitations of the technique are realized, orcein staining of liver tissue from hepatocellular carcinoma patients may prove useful for retrospective screening surveys to assess the prevalence of HBAg positivity in these patients.</description><identifier>ISSN: 0008-543X</identifier><identifier>EISSN: 1097-0142</identifier><identifier>DOI: 10.1002/1097-0142(197801)41:1<245::AID-CNCR2820410134>3.0.CO;2-4</identifier><identifier>PMID: 75053</identifier><language>eng</language><publisher>New York: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>African Continental Ancestry Group ; Carcinoma, Hepatocellular - immunology ; Diagnostic Errors ; Hemagglutination Tests ; Hepatitis B Antigens - analysis ; Hepatitis B Surface Antigens - analysis ; Humans ; Liver - immunology ; Liver Neoplasms - immunology ; Male ; Radioimmunoassay ; Serologic Tests - methods ; Staining and Labeling</subject><ispartof>Cancer, 1978-01, Vol.41 (1), p.245-249</ispartof><rights>Copyright © 1978 American Cancer Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/75053$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cohen, Cynthia</creatorcontrib><creatorcontrib>Berson, S. D.</creatorcontrib><creatorcontrib>Geddes, E. W.</creatorcontrib><title>Hepatitis B antigen in black patients with hepatocellular carcinoma. Correlation between orcein stained liver sections and serology</title><title>Cancer</title><addtitle>Cancer</addtitle><description>Formalin‐fixed paraffin‐embedded autopsy tissue of liver and tumor from 50 male black mineworkers with hepatocellular carcinoma were examined by orcein stain for the presence of cytoplasmic hepatitis B surface antigen. The results were correlated with the serum hepatitis B antigen (HBAg). In 72% serum HBAg was positive. Orcein staining of nontumor liver cell cytoplasm was present in 18 (36%). Sixteen (89%) of these orcein‐positive cases were serum HBAg positive. The two false negative serum HBAg results were obtained by immunodiffusion, immunoelectrophoresis and complement fixation. Serum HBAg, measured by radio‐immunoassay and hemagglutination, was positive in 14 orcein‐negative cases. Six other negative orcein results appeared to be due to sampling error. Orcein staining was noted in tumor cells of three serum HBAg positive patients. Provided the limitations of the technique are realized, orcein staining of liver tissue from hepatocellular carcinoma patients may prove useful for retrospective screening surveys to assess the prevalence of HBAg positivity in these patients.</description><subject>African Continental Ancestry Group</subject><subject>Carcinoma, Hepatocellular - immunology</subject><subject>Diagnostic Errors</subject><subject>Hemagglutination Tests</subject><subject>Hepatitis B Antigens - analysis</subject><subject>Hepatitis B Surface Antigens - analysis</subject><subject>Humans</subject><subject>Liver - immunology</subject><subject>Liver Neoplasms - immunology</subject><subject>Male</subject><subject>Radioimmunoassay</subject><subject>Serologic Tests - methods</subject><subject>Staining and Labeling</subject><issn>0008-543X</issn><issn>1097-0142</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1978</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkUtv1DAUhS1EBUPhF7DxCsEig1-JnaFClPBopYqREEjsrhznpjVk4sHOMJo1fxyHqSrB6urqnHssf4eQN5wtOWPiJWe1LhhX4jmvtWH8heIrfiZUuVqdX74rmk_NZ2EEU5xxqV7LJVs261eiUPfI4u70PlkwxkxRKvntIXmU0ve8alHKB-REl6yUC_L7Ard28pNP9C214-SvcaR-pO1g3Q86SzhOie79dENvZmtwOAy7wUbqbHR-DBu7pE2IEYdsDvkSpz3mkBAd5qA0WT9iRwf_CyNN6GZTyk91eYlhCNeHx-Skt0PCJ7fzlHz98P5Lc1FcrT9eNudXxVbUlSq0rfqe87oSqI3ple2d4Va2uratdsrkWbWV7BTrkMtSKKuFVqK2ihuGmcEpeXbM3cbwc4dpgo1P83fsiGGXwEitM9AyG5_eGnftBjvYRr-x8QB_mWXVHdW9H_BwJ3IGc3Ew04eZPhyLA8WBQy4Ocm_wb28ggUGzBgHqP0X-Aai6lBI</recordid><startdate>197801</startdate><enddate>197801</enddate><creator>Cohen, Cynthia</creator><creator>Berson, S. D.</creator><creator>Geddes, E. W.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>197801</creationdate><title>Hepatitis B antigen in black patients with hepatocellular carcinoma. Correlation between orcein stained liver sections and serology</title><author>Cohen, Cynthia ; Berson, S. D. ; Geddes, E. W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p2964-7a6ff11962e788f4afc81a3b79ab7c4879a6b63d40de13524a727429a4180e543</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1978</creationdate><topic>African Continental Ancestry Group</topic><topic>Carcinoma, Hepatocellular - immunology</topic><topic>Diagnostic Errors</topic><topic>Hemagglutination Tests</topic><topic>Hepatitis B Antigens - analysis</topic><topic>Hepatitis B Surface Antigens - analysis</topic><topic>Humans</topic><topic>Liver - immunology</topic><topic>Liver Neoplasms - immunology</topic><topic>Male</topic><topic>Radioimmunoassay</topic><topic>Serologic Tests - methods</topic><topic>Staining and Labeling</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cohen, Cynthia</creatorcontrib><creatorcontrib>Berson, S. D.</creatorcontrib><creatorcontrib>Geddes, E. W.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Cancer</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cohen, Cynthia</au><au>Berson, S. D.</au><au>Geddes, E. W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hepatitis B antigen in black patients with hepatocellular carcinoma. Correlation between orcein stained liver sections and serology</atitle><jtitle>Cancer</jtitle><addtitle>Cancer</addtitle><date>1978-01</date><risdate>1978</risdate><volume>41</volume><issue>1</issue><spage>245</spage><epage>249</epage><pages>245-249</pages><issn>0008-543X</issn><eissn>1097-0142</eissn><abstract>Formalin‐fixed paraffin‐embedded autopsy tissue of liver and tumor from 50 male black mineworkers with hepatocellular carcinoma were examined by orcein stain for the presence of cytoplasmic hepatitis B surface antigen. The results were correlated with the serum hepatitis B antigen (HBAg). In 72% serum HBAg was positive. Orcein staining of nontumor liver cell cytoplasm was present in 18 (36%). Sixteen (89%) of these orcein‐positive cases were serum HBAg positive. The two false negative serum HBAg results were obtained by immunodiffusion, immunoelectrophoresis and complement fixation. Serum HBAg, measured by radio‐immunoassay and hemagglutination, was positive in 14 orcein‐negative cases. Six other negative orcein results appeared to be due to sampling error. Orcein staining was noted in tumor cells of three serum HBAg positive patients. Provided the limitations of the technique are realized, orcein staining of liver tissue from hepatocellular carcinoma patients may prove useful for retrospective screening surveys to assess the prevalence of HBAg positivity in these patients.</abstract><cop>New York</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>75053</pmid><doi>10.1002/1097-0142(197801)41:1<245::AID-CNCR2820410134>3.0.CO;2-4</doi><tpages>5</tpages></addata></record> |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | African Continental Ancestry Group Carcinoma, Hepatocellular - immunology Diagnostic Errors Hemagglutination Tests Hepatitis B Antigens - analysis Hepatitis B Surface Antigens - analysis Humans Liver - immunology Liver Neoplasms - immunology Male Radioimmunoassay Serologic Tests - methods Staining and Labeling |
| title | Hepatitis B antigen in black patients with hepatocellular carcinoma. Correlation between orcein stained liver sections and serology |
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