Cooperative binding of concanavalin a to thymocyte membrane vesicles augments uptake of α-aminoisobutyrate

Cooperative binding of concanavalin a to thymocyte membrane vesicles augments uptake of α-aminoisobutyrate

(1) Membrane vesicles from rabbit thymocytes accumulate α‐aminoisobutyrate in the presence of 0.1 M NaCl. Uptake is 1/2 maximal after about 2 min and reaches a plateau value (61 pmoles/mg protein) after 30 min. (2) Up to 25 μg concanavalin A/ml, binding of the lectin describes a sigmoid curve indica...

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Veröffentlicht in:Journal of cellular physiology 1976-12, Vol.89 (4), p.775-777
Hauptverfasser: Schmidt-Ullrich, Rupert, Mann, Nathan D., Wallach, Donald F. H.
Format: Artikel
Sprache:eng
Schlagworte:
Aminoisobutyric Acids - metabolism
Animals
Cell Membrane - metabolism
Concanavalin A - metabolism
Protein Binding
Rabbits
Sodium Chloride - pharmacology
Thymus Gland - cytology
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container_title Journal of cellular physiology
container_volume 89
creator Schmidt-Ullrich, Rupert
Mann, Nathan D.
Wallach, Donald F. H.
description (1) Membrane vesicles from rabbit thymocytes accumulate α‐aminoisobutyrate in the presence of 0.1 M NaCl. Uptake is 1/2 maximal after about 2 min and reaches a plateau value (61 pmoles/mg protein) after 30 min. (2) Up to 25 μg concanavalin A/ml, binding of the lectin describes a sigmoid curve indicative of a cooperative process. (3) At lectin concentrations up to 8 μg/ml, lectin binding enhances the uptake of α‐aminoisobutyrate (maximally 30%).
doi_str_mv 10.1002/jcp.1040890442
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source MEDLINE; Wiley Online Library Journals Frontfile Complete
subjects Aminoisobutyric Acids - metabolism
Animals
Cell Membrane - metabolism
Concanavalin A - metabolism
Protein Binding
Rabbits
Sodium Chloride - pharmacology
Thymus Gland - cytology
title Cooperative binding of concanavalin a to thymocyte membrane vesicles augments uptake of α-aminoisobutyrate
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