Absence of the membrane marker enzyme 5′-nucleotidase in human polymorphonuclear leukocytes

As a first step in an attempt to purify the plasma membrane from human polymorphonuclear leukocytes (PMNL), we initiated a search for a marker enzyme in order to monitor the course of the purification procedure. Nucleoside-5′-monophosphatase (5′-nucleotidase) was first used as a plasma membrane mark...

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Veröffentlicht in:Biochemical medicine 1976-06, Vol.15 (3), p.289-295
Hauptverfasser: Shirley, Pamela S., Wang, Patsy, DeChatelet, Lawrence R., Waite, Moseley
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Sprache:eng
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