The use of agarose gels for the assay of the deoxyribonuclease activity associated with the soluble penton of adenoviruses
Evidence is presented that the excess pool of penton complexes purified from adenovirus type 1-infected HEp-2 cells also carry an endonucleolytic activity. The effect was measured with both E. coli and adenovirus type 1 double-stranded DNA substrates. The use of agarose gels for assaying the endonuc...
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| Veröffentlicht in: | Archives of virology 1976-01, Vol.52 (4), p.315-321 |
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| container_title | Archives of virology |
| container_volume | 52 |
| creator | Medveczky, P Csuzi, S Nász, I Adám, E Berencsi, G Antoni, F |
| description | Evidence is presented that the excess pool of penton complexes purified from adenovirus type 1-infected HEp-2 cells also carry an endonucleolytic activity. The effect was measured with both E. coli and adenovirus type 1 double-stranded DNA substrates. The use of agarose gels for assaying the endonuclease activity allowed the approximative calculation of the number of penton molecules which might produce one DNA double-strand break under the experimental conditions selected. |
| doi_str_mv | 10.1007/BF01315620 |
| format | Article |
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| fulltext | fulltext |
| identifier | ISSN: 0304-8608 |
| ispartof | Archives of virology, 1976-01, Vol.52 (4), p.315-321 |
| issn | 0304-8608 1432-8798 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_83631480 |
| source | MEDLINE; SpringerNature Journals |
| subjects | Adenoviruses, Human - enzymology Adenoviruses, Human - metabolism Deoxyribonucleases - metabolism DNA, Bacterial - metabolism DNA, Viral - metabolism Electrophoresis, Agar Gel Endonucleases - metabolism Escherichia coli - metabolism Hydrolysis Solubility Viral Proteins - metabolism |
| title | The use of agarose gels for the assay of the deoxyribonuclease activity associated with the soluble penton of adenoviruses |
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