Ultrastructural details of Sertoli cell junctional complexes in vivo and their modifications in tissue culture
This study was carried out using thin sections, lanthanum tracing, and freeze-cleaving in order to investigate the Sertoli cell junctions of rat testis in vivo and their maintenance in culture. The presence of a new type of membrane specialization has been revealed. This consists of a close associat...
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| Veröffentlicht in: | Cell and tissue research 1976-09, Vol.172 (1), p.29-38 |
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| container_title | Cell and tissue research |
| container_volume | 172 |
| creator | Bigliardi, E Talluri, M V |
| description | This study was carried out using thin sections, lanthanum tracing, and freeze-cleaving in order to investigate the Sertoli cell junctions of rat testis in vivo and their maintenance in culture. The presence of a new type of membrane specialization has been revealed. This consists of a close association between tight junctions and nexuses. The Sertoli cell contact specializations show a progressive disorganization in vitro correlated with the duration of the period in culture. The relationship between the morphological changes in Sertoli cell junctions and the lack of spermatogenesis in culture is discussed. |
| doi_str_mv | 10.1007/BF00226047 |
| format | Article |
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The presence of a new type of membrane specialization has been revealed. This consists of a close association between tight junctions and nexuses. The Sertoli cell contact specializations show a progressive disorganization in vitro correlated with the duration of the period in culture. 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The presence of a new type of membrane specialization has been revealed. This consists of a close association between tight junctions and nexuses. The Sertoli cell contact specializations show a progressive disorganization in vitro correlated with the duration of the period in culture. The relationship between the morphological changes in Sertoli cell junctions and the lack of spermatogenesis in culture is discussed.</description><subject>Animals</subject><subject>Culture Techniques</subject><subject>Freeze Fracturing</subject><subject>Intercellular Junctions - ultrastructure</subject><subject>Lanthanum</subject><subject>Male</subject><subject>Microscopy, Electron</subject><subject>Permeability</subject><subject>Rats</subject><subject>Sertoli Cells - ultrastructure</subject><subject>Spermatogenesis</subject><issn>0302-766X</issn><issn>1432-0878</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1976</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkDtPwzAURi3EqxQWZgZPDEiBazsPe4SKl1SJASqxRa5zI4ycuNgOgn9PSiuY7nCPjvQdQk4ZXDKA6urmDoDzEvJqh0xYLngGspK7ZAICeFaV5eshOYrxHYDlZakOyL5SjIOYkH7hUtAxhcGkIWhHG0zaukh9S58xJO8sNegcfR96k6zvR8T4buXwCyO1Pf20n57qvqHpDW2gnW9sa41eo7__ZGMckJrBjX48JnutdhFPtndKFne3L7OHbP50_zi7nmeGqTJlvJKyYJozBYiNKBRKqVQOJWBreFPIqioaQCi0AFjqXAttmBZSaM2hUZWYkvONdxX8x4Ax1Z2N6x26Rz_EWoqSq9E-ghcb0AQfY8C2XgXb6fBdM6jXbev_tiN8trUOyw6bP3QTU_wAG9l1Qw</recordid><startdate>19760906</startdate><enddate>19760906</enddate><creator>Bigliardi, E</creator><creator>Talluri, M V</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19760906</creationdate><title>Ultrastructural details of Sertoli cell junctional complexes in vivo and their modifications in tissue culture</title><author>Bigliardi, E ; Talluri, M V</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c196t-278851a2190eed359e88994060efc2d58775d0e05a300ba4a3ac1a383aa20d973</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1976</creationdate><topic>Animals</topic><topic>Culture Techniques</topic><topic>Freeze Fracturing</topic><topic>Intercellular Junctions - ultrastructure</topic><topic>Lanthanum</topic><topic>Male</topic><topic>Microscopy, Electron</topic><topic>Permeability</topic><topic>Rats</topic><topic>Sertoli Cells - ultrastructure</topic><topic>Spermatogenesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bigliardi, E</creatorcontrib><creatorcontrib>Talluri, M V</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cell and tissue research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bigliardi, E</au><au>Talluri, M V</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ultrastructural details of Sertoli cell junctional complexes in vivo and their modifications in tissue culture</atitle><jtitle>Cell and tissue research</jtitle><addtitle>Cell Tissue Res</addtitle><date>1976-09-06</date><risdate>1976</risdate><volume>172</volume><issue>1</issue><spage>29</spage><epage>38</epage><pages>29-38</pages><issn>0302-766X</issn><eissn>1432-0878</eissn><abstract>This study was carried out using thin sections, lanthanum tracing, and freeze-cleaving in order to investigate the Sertoli cell junctions of rat testis in vivo and their maintenance in culture. 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| ispartof | Cell and tissue research, 1976-09, Vol.172 (1), p.29-38 |
| issn | 0302-766X 1432-0878 |
| language | eng |
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| subjects | Animals Culture Techniques Freeze Fracturing Intercellular Junctions - ultrastructure Lanthanum Male Microscopy, Electron Permeability Rats Sertoli Cells - ultrastructure Spermatogenesis |
| title | Ultrastructural details of Sertoli cell junctional complexes in vivo and their modifications in tissue culture |
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