A sensitive fluorometric method for the determination of aliphatic diamines and polyamines in biological materials by high-speed liquid chromatography
A high-speed liquid chromatographic method for the determination of fluores-camine-labeled diamines and polyamines was applied to rat liver, human serum, and urine. The samples, after addition of 1,6-hexanediamine and N-3-aminopropyl-1,6-hexanediamine as internal standards, were appropriately pretre...
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| Veröffentlicht in: | Analytical biochemistry 1976-12, Vol.76 (2), p.392-406 |
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| container_end_page | 406 |
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| container_title | Analytical biochemistry |
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| creator | Samejima, Keijiro Kawase, Masako Sakamoto, Shunji Okada, Masashi Endo, Yasuo |
| description | A high-speed liquid chromatographic method for the determination of fluores-camine-labeled diamines and polyamines was applied to rat liver, human serum, and urine. The samples, after addition of 1,6-hexanediamine and
N-3-aminopropyl-1,6-hexanediamine as internal standards, were appropriately pretreated by perchloric acid precipitation and/or acid hydrolysis. The resultant filtrates were subjected to CM-cellulose column chromatography in pyridine-acetic acid buffer. Stepwise elution with 0.2 and 1
m buffer gave fairly pure diamine and polyamine fractions. The evaporated fractions were dissolved in borate buffer and reacted with fluorescamine by either Procedure I or II, depending on the amine content. Aliquots of the reaction mixture were subjected to high-speed liquid chromatography for measurement of the amines. It was possible by this method to assay biological samples containing less than 100 pmol of the amines with about 5% relative standard deviation. |
| doi_str_mv | 10.1016/0003-2697(76)90334-1 |
| format | Article |
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m buffer gave fairly pure diamine and polyamine fractions. The evaporated fractions were dissolved in borate buffer and reacted with fluorescamine by either Procedure I or II, depending on the amine content. Aliquots of the reaction mixture were subjected to high-speed liquid chromatography for measurement of the amines. It was possible by this method to assay biological samples containing less than 100 pmol of the amines with about 5% relative standard deviation.</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1016/0003-2697(76)90334-1</identifier><identifier>PMID: 998982</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Chromatography, High Pressure Liquid ; Diamines - analysis ; Diamines - blood ; Diamines - urine ; Fluorescamine ; Humans ; Liver - analysis ; Methods ; Polyamines - analysis ; Polyamines - blood ; Polyamines - urine ; Rats ; Spectrometry, Fluorescence</subject><ispartof>Analytical biochemistry, 1976-12, Vol.76 (2), p.392-406</ispartof><rights>1976</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c422t-a1e57bb2c983f6c66911350f57896f579ec6141b7b3e752d1a4e6af60d4091c3</citedby><cites>FETCH-LOGICAL-c422t-a1e57bb2c983f6c66911350f57896f579ec6141b7b3e752d1a4e6af60d4091c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0003-2697(76)90334-1$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3549,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/998982$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Samejima, Keijiro</creatorcontrib><creatorcontrib>Kawase, Masako</creatorcontrib><creatorcontrib>Sakamoto, Shunji</creatorcontrib><creatorcontrib>Okada, Masashi</creatorcontrib><creatorcontrib>Endo, Yasuo</creatorcontrib><title>A sensitive fluorometric method for the determination of aliphatic diamines and polyamines in biological materials by high-speed liquid chromatography</title><title>Analytical biochemistry</title><addtitle>Anal Biochem</addtitle><description>A high-speed liquid chromatographic method for the determination of fluores-camine-labeled diamines and polyamines was applied to rat liver, human serum, and urine. The samples, after addition of 1,6-hexanediamine and
N-3-aminopropyl-1,6-hexanediamine as internal standards, were appropriately pretreated by perchloric acid precipitation and/or acid hydrolysis. The resultant filtrates were subjected to CM-cellulose column chromatography in pyridine-acetic acid buffer. Stepwise elution with 0.2 and 1
m buffer gave fairly pure diamine and polyamine fractions. The evaporated fractions were dissolved in borate buffer and reacted with fluorescamine by either Procedure I or II, depending on the amine content. Aliquots of the reaction mixture were subjected to high-speed liquid chromatography for measurement of the amines. It was possible by this method to assay biological samples containing less than 100 pmol of the amines with about 5% relative standard deviation.</description><subject>Animals</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Diamines - analysis</subject><subject>Diamines - blood</subject><subject>Diamines - urine</subject><subject>Fluorescamine</subject><subject>Humans</subject><subject>Liver - analysis</subject><subject>Methods</subject><subject>Polyamines - analysis</subject><subject>Polyamines - blood</subject><subject>Polyamines - urine</subject><subject>Rats</subject><subject>Spectrometry, Fluorescence</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1976</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UU1r3DAQFaVf27T_IAedSnpwI1m2ZF0KIaQfEMgldyFL4_UU2XIkO7B_pL-32u6SYy_zGN6bN8wbQi45-8oZl9eMMVHVUqsrJb9oJkRT8Vdkx5mWFRNMvya7F8l78iHn34xx3rTyHXmrdae7ekf-3NAMc8YVn4EOYYspTrAmdLTAGD0dYqLrCNTDCmnC2a4YZxoHagMuY-kc9WgLAZna2dMlhsO5xZn2GEPco7OBTrYYoA2Z9gc64n6s8gLgacCnDT11Y9ls17hPdhkPH8mboUjh0xkvyOP3u8fbn9X9w49ftzf3lWvqeq0sh1b1fe10JwbppNSci5YNreq0LFWDk7zhveoFqLb23DYg7SCZb5jmTlyQzyfbJcWnDfJqJswOQrAzxC2bTkiupGJF2JyELsWcEwxmSTjZdDCcmeMzzDFpc0zaKGn-PcPwMnZ59t_6CfzL0Cn9Qn870VBOfEZIJjuE2YHHBG41PuL__f8C4AqcUQ</recordid><startdate>197612</startdate><enddate>197612</enddate><creator>Samejima, Keijiro</creator><creator>Kawase, Masako</creator><creator>Sakamoto, Shunji</creator><creator>Okada, Masashi</creator><creator>Endo, Yasuo</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197612</creationdate><title>A sensitive fluorometric method for the determination of aliphatic diamines and polyamines in biological materials by high-speed liquid chromatography</title><author>Samejima, Keijiro ; Kawase, Masako ; Sakamoto, Shunji ; Okada, Masashi ; Endo, Yasuo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-a1e57bb2c983f6c66911350f57896f579ec6141b7b3e752d1a4e6af60d4091c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1976</creationdate><topic>Animals</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Diamines - analysis</topic><topic>Diamines - blood</topic><topic>Diamines - urine</topic><topic>Fluorescamine</topic><topic>Humans</topic><topic>Liver - analysis</topic><topic>Methods</topic><topic>Polyamines - analysis</topic><topic>Polyamines - blood</topic><topic>Polyamines - urine</topic><topic>Rats</topic><topic>Spectrometry, Fluorescence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Samejima, Keijiro</creatorcontrib><creatorcontrib>Kawase, Masako</creatorcontrib><creatorcontrib>Sakamoto, Shunji</creatorcontrib><creatorcontrib>Okada, Masashi</creatorcontrib><creatorcontrib>Endo, Yasuo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Samejima, Keijiro</au><au>Kawase, Masako</au><au>Sakamoto, Shunji</au><au>Okada, Masashi</au><au>Endo, Yasuo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A sensitive fluorometric method for the determination of aliphatic diamines and polyamines in biological materials by high-speed liquid chromatography</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>1976-12</date><risdate>1976</risdate><volume>76</volume><issue>2</issue><spage>392</spage><epage>406</epage><pages>392-406</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>A high-speed liquid chromatographic method for the determination of fluores-camine-labeled diamines and polyamines was applied to rat liver, human serum, and urine. The samples, after addition of 1,6-hexanediamine and
N-3-aminopropyl-1,6-hexanediamine as internal standards, were appropriately pretreated by perchloric acid precipitation and/or acid hydrolysis. The resultant filtrates were subjected to CM-cellulose column chromatography in pyridine-acetic acid buffer. Stepwise elution with 0.2 and 1
m buffer gave fairly pure diamine and polyamine fractions. The evaporated fractions were dissolved in borate buffer and reacted with fluorescamine by either Procedure I or II, depending on the amine content. Aliquots of the reaction mixture were subjected to high-speed liquid chromatography for measurement of the amines. It was possible by this method to assay biological samples containing less than 100 pmol of the amines with about 5% relative standard deviation.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>998982</pmid><doi>10.1016/0003-2697(76)90334-1</doi><tpages>15</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Analytical biochemistry, 1976-12, Vol.76 (2), p.392-406 |
| issn | 0003-2697 1096-0309 |
| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Chromatography, High Pressure Liquid Diamines - analysis Diamines - blood Diamines - urine Fluorescamine Humans Liver - analysis Methods Polyamines - analysis Polyamines - blood Polyamines - urine Rats Spectrometry, Fluorescence |
| title | A sensitive fluorometric method for the determination of aliphatic diamines and polyamines in biological materials by high-speed liquid chromatography |
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