Dehydroepiandrosterone sulfate in plasma: hydrolysis, extraction and radioimmunoassay
At pH 4.5, the hydrolysis of 3 β-hydroxy-5-androsten-17-one 3-sulfate (DHA-SO 4) to DHA was complete within 75 min at 120° or 4h at 100°. In the same conditions, the 3 α-SO 4 of androsterone was stable, and only 8.5% of the 3 α-SO 4 of epiandrosterone hydrolysed to epiandrosterone. Of the sulfates o...
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| Veröffentlicht in: | Steroids 1976-09, Vol.28 (3), p.311-324 |
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| creator | Metcalf, Mary G. |
| description | At pH 4.5, the hydrolysis of 3
β-hydroxy-5-androsten-17-one 3-sulfate (DHA-SO
4) to DHA was complete within 75 min at 120° or 4h at 100°. In the same conditions, the 3
α-SO
4 of androsterone was stable, and only 8.5% of the 3
α-SO
4 of epiandrosterone hydrolysed to epiandrosterone. Of the sulfates of 5-androstene-3
β,17
β-diol, 100% of the 3
β-mono-SO
4, 2% of the 17
β-mono-SO
4 and none of the 3
β,17
β-di-SO
4 was converted to 5-androstenediol.
Denatured plasma proteins adsorbed DHA. The recovery of DHA from plasma diluted 1:100 before hydrolysis was 91.0 ± 3.5% and from plasma diluted 1:10, 58.7 ± 6.2% (mean ± S.D.). In similar conditions the recovery of cholesterol from plasma diluted 1:20, was 0.12 − 1.76% (mean, 0.44%).
A radioimmunoassay for DHA in extracts of hydrolysed plasma is described. Results for normal subjects in the age range 17–45y were 192 ± 73
μg/dl (22 men) and 158 ± 57
μg/dl (40 women). |
| doi_str_mv | 10.1016/0039-128X(76)90042-8 |
| format | Article |
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β-hydroxy-5-androsten-17-one 3-sulfate (DHA-SO
4) to DHA was complete within 75 min at 120° or 4h at 100°. In the same conditions, the 3
α-SO
4 of androsterone was stable, and only 8.5% of the 3
α-SO
4 of epiandrosterone hydrolysed to epiandrosterone. Of the sulfates of 5-androstene-3
β,17
β-diol, 100% of the 3
β-mono-SO
4, 2% of the 17
β-mono-SO
4 and none of the 3
β,17
β-di-SO
4 was converted to 5-androstenediol.
Denatured plasma proteins adsorbed DHA. The recovery of DHA from plasma diluted 1:100 before hydrolysis was 91.0 ± 3.5% and from plasma diluted 1:10, 58.7 ± 6.2% (mean ± S.D.). In similar conditions the recovery of cholesterol from plasma diluted 1:20, was 0.12 − 1.76% (mean, 0.44%).
A radioimmunoassay for DHA in extracts of hydrolysed plasma is described. Results for normal subjects in the age range 17–45y were 192 ± 73
μg/dl (22 men) and 158 ± 57
μg/dl (40 women).</description><identifier>ISSN: 0039-128X</identifier><identifier>EISSN: 1878-5867</identifier><identifier>DOI: 10.1016/0039-128X(76)90042-8</identifier><identifier>PMID: 136071</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adult ; Blood Proteins ; Cholesterol - blood ; Dehydroepiandrosterone - analogs & derivatives ; Dehydroepiandrosterone - blood ; Dehydroepiandrosterone - isolation & purification ; Female ; Humans ; Hydrolysis ; Kinetics ; Male ; Middle Aged ; Protein Binding ; Radioimmunoassay - methods ; Sex Factors</subject><ispartof>Steroids, 1976-09, Vol.28 (3), p.311-324</ispartof><rights>1976</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-f40983c3854056a4e1b39af815631f0c2db810e688d129e4cd7e13de7aa682b43</citedby><cites>FETCH-LOGICAL-c356t-f40983c3854056a4e1b39af815631f0c2db810e688d129e4cd7e13de7aa682b43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0039-128X(76)90042-8$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/136071$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Metcalf, Mary G.</creatorcontrib><title>Dehydroepiandrosterone sulfate in plasma: hydrolysis, extraction and radioimmunoassay</title><title>Steroids</title><addtitle>Steroids</addtitle><description>At pH 4.5, the hydrolysis of 3
β-hydroxy-5-androsten-17-one 3-sulfate (DHA-SO
4) to DHA was complete within 75 min at 120° or 4h at 100°. In the same conditions, the 3
α-SO
4 of androsterone was stable, and only 8.5% of the 3
α-SO
4 of epiandrosterone hydrolysed to epiandrosterone. Of the sulfates of 5-androstene-3
β,17
β-diol, 100% of the 3
β-mono-SO
4, 2% of the 17
β-mono-SO
4 and none of the 3
β,17
β-di-SO
4 was converted to 5-androstenediol.
Denatured plasma proteins adsorbed DHA. The recovery of DHA from plasma diluted 1:100 before hydrolysis was 91.0 ± 3.5% and from plasma diluted 1:10, 58.7 ± 6.2% (mean ± S.D.). In similar conditions the recovery of cholesterol from plasma diluted 1:20, was 0.12 − 1.76% (mean, 0.44%).
A radioimmunoassay for DHA in extracts of hydrolysed plasma is described. Results for normal subjects in the age range 17–45y were 192 ± 73
μg/dl (22 men) and 158 ± 57
μg/dl (40 women).</description><subject>Adult</subject><subject>Blood Proteins</subject><subject>Cholesterol - blood</subject><subject>Dehydroepiandrosterone - analogs & derivatives</subject><subject>Dehydroepiandrosterone - blood</subject><subject>Dehydroepiandrosterone - isolation & purification</subject><subject>Female</subject><subject>Humans</subject><subject>Hydrolysis</subject><subject>Kinetics</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Protein Binding</subject><subject>Radioimmunoassay - methods</subject><subject>Sex Factors</subject><issn>0039-128X</issn><issn>1878-5867</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1976</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtKxDAYhYN4G0ffYBZdiYLVpGnT1IUg4xUG3DjgLqTJX4y0TU1asW9v5oLuXJ3FucD5EJoRfEkwYVcY0yImCX87y9l5gXGaxHwHTQjPeZxxlu-iyW_kEB15_4ExZrRIDtA-oQznZIKWd_A-amehM7IN6ntwtoXID3Ule4hMG3W19I28jta5evTGX0Tw3TupemPbKNQiJ7WxpmmG1krv5XiM9ipZezjZ6hQtH-5f50_x4uXxeX67iBXNWB9XKS44VZRnKc6YTIGUtJAVJxmjpMIq0SUnGBjnmiQFpErnQKiGXErGkzKlU3S62e2c_RzA96IxXkFdyxbs4AUPL0lGcAimm6AKF72DSnTONNKNgmCxgilWpMSKlMiZWMMM7SmabfeHsgH9V1rTC_bNxobw8cuAE14ZaBVo40D1Qlvz__4PZDyFFA</recordid><startdate>197609</startdate><enddate>197609</enddate><creator>Metcalf, Mary G.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197609</creationdate><title>Dehydroepiandrosterone sulfate in plasma: hydrolysis, extraction and radioimmunoassay</title><author>Metcalf, Mary G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-f40983c3854056a4e1b39af815631f0c2db810e688d129e4cd7e13de7aa682b43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1976</creationdate><topic>Adult</topic><topic>Blood Proteins</topic><topic>Cholesterol - blood</topic><topic>Dehydroepiandrosterone - analogs & derivatives</topic><topic>Dehydroepiandrosterone - blood</topic><topic>Dehydroepiandrosterone - isolation & purification</topic><topic>Female</topic><topic>Humans</topic><topic>Hydrolysis</topic><topic>Kinetics</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Protein Binding</topic><topic>Radioimmunoassay - methods</topic><topic>Sex Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Metcalf, Mary G.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Steroids</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Metcalf, Mary G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dehydroepiandrosterone sulfate in plasma: hydrolysis, extraction and radioimmunoassay</atitle><jtitle>Steroids</jtitle><addtitle>Steroids</addtitle><date>1976-09</date><risdate>1976</risdate><volume>28</volume><issue>3</issue><spage>311</spage><epage>324</epage><pages>311-324</pages><issn>0039-128X</issn><eissn>1878-5867</eissn><abstract>At pH 4.5, the hydrolysis of 3
β-hydroxy-5-androsten-17-one 3-sulfate (DHA-SO
4) to DHA was complete within 75 min at 120° or 4h at 100°. In the same conditions, the 3
α-SO
4 of androsterone was stable, and only 8.5% of the 3
α-SO
4 of epiandrosterone hydrolysed to epiandrosterone. Of the sulfates of 5-androstene-3
β,17
β-diol, 100% of the 3
β-mono-SO
4, 2% of the 17
β-mono-SO
4 and none of the 3
β,17
β-di-SO
4 was converted to 5-androstenediol.
Denatured plasma proteins adsorbed DHA. The recovery of DHA from plasma diluted 1:100 before hydrolysis was 91.0 ± 3.5% and from plasma diluted 1:10, 58.7 ± 6.2% (mean ± S.D.). In similar conditions the recovery of cholesterol from plasma diluted 1:20, was 0.12 − 1.76% (mean, 0.44%).
A radioimmunoassay for DHA in extracts of hydrolysed plasma is described. Results for normal subjects in the age range 17–45y were 192 ± 73
μg/dl (22 men) and 158 ± 57
μg/dl (40 women).</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>136071</pmid><doi>10.1016/0039-128X(76)90042-8</doi><tpages>14</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0039-128X |
| ispartof | Steroids, 1976-09, Vol.28 (3), p.311-324 |
| issn | 0039-128X 1878-5867 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Adult Blood Proteins Cholesterol - blood Dehydroepiandrosterone - analogs & derivatives Dehydroepiandrosterone - blood Dehydroepiandrosterone - isolation & purification Female Humans Hydrolysis Kinetics Male Middle Aged Protein Binding Radioimmunoassay - methods Sex Factors |
| title | Dehydroepiandrosterone sulfate in plasma: hydrolysis, extraction and radioimmunoassay |
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