Purification and properties of Amycolatopsis mediterranei DSM 43304 lipase and its potential in flavour ester synthesis

An extracellular thermostable lipase from Amycolatopsis mediterranei DSM 43304 has been purified to homogeneity using ammonium sulphate precipitation followed by anion exchange chromatography and hydrophobic interaction chromatography. This protocol resulted in a 398-fold purification with 36% final...

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Veröffentlicht in:	Bioresource technology 2011-02, Vol.102 (3), p.3373-3379
Hauptverfasser:	Dheeman, Dharmendra S., Henehan, Gary T.M., Frías, Jesus M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Actinomycetales - enzymology Actinomycete lipase Amino Acid Sequence Amycolatopsis mediterranei Biological and medical sciences Characterization Enzyme Activation Enzyme Stability Ester synthesis Esterification Esters Fundamental and applied biological sciences. Psychology Lipase - chemistry Lipase - isolation & purification Lipase - metabolism Molecular Sequence Data Pentanols - chemistry Purification Substrate Specificity
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