Effect of 5-bromodeoxyuridine on the appearance of cytoplasmic poly-A containing RNA
Cytoplasmic poly‐A containing RNA, synthesized by cultured chick embryo cells, was examined during growth in 5‐bromodeoxyuridine. The kinetics of 3H‐adenosine incorporation into this species of RNA, when compared to the rest of the cytoplasmic RNA, and to control cells, indicates that the rate of sy...
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| Veröffentlicht in: | Journal of cellular physiology 1976-09, Vol.89 (1), p.19-27 |
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| container_title | Journal of cellular physiology |
| container_volume | 89 |
| creator | Pawlowski, P.J |
| description | Cytoplasmic poly‐A containing RNA, synthesized by cultured chick embryo cells, was examined during growth in 5‐bromodeoxyuridine. The kinetics of 3H‐adenosine incorporation into this species of RNA, when compared to the rest of the cytoplasmic RNA, and to control cells, indicates that the rate of synthesis of this RNA is slower in BrdU treated cells. An examination of the rate at which a steady state distribution of radioactivity, between the poly‐A segment and non‐poly‐A portion of poly‐A containing RNA is reached also indicated that this species is synthesized at a lower rate in BrdU treated cells. |
| doi_str_mv | 10.1002/jcp.1040890103 |
| format | Article |
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The kinetics of 3H‐adenosine incorporation into this species of RNA, when compared to the rest of the cytoplasmic RNA, and to control cells, indicates that the rate of synthesis of this RNA is slower in BrdU treated cells. An examination of the rate at which a steady state distribution of radioactivity, between the poly‐A segment and non‐poly‐A portion of poly‐A containing RNA is reached also indicated that this species is synthesized at a lower rate in BrdU treated cells.</description><identifier>ISSN: 0021-9541</identifier><identifier>EISSN: 1097-4652</identifier><identifier>DOI: 10.1002/jcp.1040890103</identifier><identifier>PMID: 956280</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Adenosine - metabolism ; Adenosine Triphosphate - metabolism ; animal science ; Bromodeoxyuridine - pharmacology ; Cells, Cultured ; Kinetics ; livestock ; Poly A - biosynthesis ; RNA, Messenger - analysis ; RNA, Messenger - biosynthesis ; RNA, Ribosomal - biosynthesis ; RNA, Transfer - biosynthesis ; Transcription, Genetic ; zoology</subject><ispartof>Journal of cellular physiology, 1976-09, Vol.89 (1), p.19-27</ispartof><rights>Copyright © 1976 Wiley‐Liss, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4013-eff8ab34bd8e31808e3e6cebd3dd367532105aae67f2cba59c5a00e88b752bda3</citedby><cites>FETCH-LOGICAL-c4013-eff8ab34bd8e31808e3e6cebd3dd367532105aae67f2cba59c5a00e88b752bda3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjcp.1040890103$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjcp.1040890103$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/956280$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pawlowski, P.J</creatorcontrib><title>Effect of 5-bromodeoxyuridine on the appearance of cytoplasmic poly-A containing RNA</title><title>Journal of cellular physiology</title><addtitle>J. Cell. Physiol</addtitle><description>Cytoplasmic poly‐A containing RNA, synthesized by cultured chick embryo cells, was examined during growth in 5‐bromodeoxyuridine. The kinetics of 3H‐adenosine incorporation into this species of RNA, when compared to the rest of the cytoplasmic RNA, and to control cells, indicates that the rate of synthesis of this RNA is slower in BrdU treated cells. An examination of the rate at which a steady state distribution of radioactivity, between the poly‐A segment and non‐poly‐A portion of poly‐A containing RNA is reached also indicated that this species is synthesized at a lower rate in BrdU treated cells.</description><subject>Adenosine - metabolism</subject><subject>Adenosine Triphosphate - metabolism</subject><subject>animal science</subject><subject>Bromodeoxyuridine - pharmacology</subject><subject>Cells, Cultured</subject><subject>Kinetics</subject><subject>livestock</subject><subject>Poly A - biosynthesis</subject><subject>RNA, Messenger - analysis</subject><subject>RNA, Messenger - biosynthesis</subject><subject>RNA, Ribosomal - biosynthesis</subject><subject>RNA, Transfer - biosynthesis</subject><subject>Transcription, Genetic</subject><subject>zoology</subject><issn>0021-9541</issn><issn>1097-4652</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1976</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1v1DAQhi3E17Zw5YRETtzSjuPYjo-rbemHloKgFVIvluNMiksSBzsrmn-Pq1RFnHqZGWme9z08hLyjcEABisNbO6ajhEoBBfaMrCgomZeCF8_JKgE0V7ykr8lejLcAoBRjr8hLxUVRwYpcHrct2inzbcbzOvjeN-jv5l1wjRsw80M2_cTMjCOaYAaL96CdJz92JvbOZqPv5nydWT9Mxg1uuMm-XazfkBet6SK-fdj75OrT8eXmNN9-OTnbrLe5LYGyHNu2MjUr66ZCRitIE4XFumFNw4TkrKDAjUEh28LWhivLDQBWVS15UTeG7ZOPS-8Y_O8dxkn3LlrsOjOg30VdsVJKwWUCDxbQBh9jwFaPwfUmzJqCvreok0X9z2IKvH9o3tU9No_4oi291fL-4zqcnyjT55uv_1XnS9bFCe8esyb80kIyyfWPixMt5fX15yNW6G3iPyx8a7w2N8FFffW9SP6AgaBKUPYX0ceWoQ</recordid><startdate>197609</startdate><enddate>197609</enddate><creator>Pawlowski, P.J</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>197609</creationdate><title>Effect of 5-bromodeoxyuridine on the appearance of cytoplasmic poly-A containing RNA</title><author>Pawlowski, P.J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4013-eff8ab34bd8e31808e3e6cebd3dd367532105aae67f2cba59c5a00e88b752bda3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1976</creationdate><topic>Adenosine - metabolism</topic><topic>Adenosine Triphosphate - metabolism</topic><topic>animal science</topic><topic>Bromodeoxyuridine - pharmacology</topic><topic>Cells, Cultured</topic><topic>Kinetics</topic><topic>livestock</topic><topic>Poly A - biosynthesis</topic><topic>RNA, Messenger - analysis</topic><topic>RNA, Messenger - biosynthesis</topic><topic>RNA, Ribosomal - biosynthesis</topic><topic>RNA, Transfer - biosynthesis</topic><topic>Transcription, Genetic</topic><topic>zoology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pawlowski, P.J</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of cellular physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pawlowski, P.J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of 5-bromodeoxyuridine on the appearance of cytoplasmic poly-A containing RNA</atitle><jtitle>Journal of cellular physiology</jtitle><addtitle>J. Cell. Physiol</addtitle><date>1976-09</date><risdate>1976</risdate><volume>89</volume><issue>1</issue><spage>19</spage><epage>27</epage><pages>19-27</pages><issn>0021-9541</issn><eissn>1097-4652</eissn><abstract>Cytoplasmic poly‐A containing RNA, synthesized by cultured chick embryo cells, was examined during growth in 5‐bromodeoxyuridine. The kinetics of 3H‐adenosine incorporation into this species of RNA, when compared to the rest of the cytoplasmic RNA, and to control cells, indicates that the rate of synthesis of this RNA is slower in BrdU treated cells. An examination of the rate at which a steady state distribution of radioactivity, between the poly‐A segment and non‐poly‐A portion of poly‐A containing RNA is reached also indicated that this species is synthesized at a lower rate in BrdU treated cells.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>956280</pmid><doi>10.1002/jcp.1040890103</doi><tpages>9</tpages></addata></record> |
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| ispartof | Journal of cellular physiology, 1976-09, Vol.89 (1), p.19-27 |
| issn | 0021-9541 1097-4652 |
| language | eng |
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| source | MEDLINE; Access via Wiley Online Library |
| subjects | Adenosine - metabolism Adenosine Triphosphate - metabolism animal science Bromodeoxyuridine - pharmacology Cells, Cultured Kinetics livestock Poly A - biosynthesis RNA, Messenger - analysis RNA, Messenger - biosynthesis RNA, Ribosomal - biosynthesis RNA, Transfer - biosynthesis Transcription, Genetic zoology |
| title | Effect of 5-bromodeoxyuridine on the appearance of cytoplasmic poly-A containing RNA |
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