A sensitive radiochemical assay for adenosine deaminase

Adenosine deaminase (EC 3.5.4.4) (ADA) is assayed by incubating [8- 14C]adenosine with the enzyme containing solution for 2 hr at pH 7.4 and 37°C. The trichloroacetic acid protein free filtrate is acid hydrolyzed and separated on a 2.5 × 0.2 cm 2 Dowex-50 column where the hypoxanthine is eluted quan...

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Veröffentlicht in:	Clinical immunology and immunopathology 1976-03, Vol.5 (2), p.173-176
Hauptverfasser:	Mudawwar, Fuad, Jebara, Haifa, Geha, Raif, al-Khalidi, Usama
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenine - isolation & purification Adenosine Deaminase - analysis Adenosine Deaminase - blood Humans Hypoxanthines - isolation & purification Nucleoside Deaminases - analysis Radiochemistry
Online-Zugang:	Volltext
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